[1]张圆圆,刘文敬,张斌斌,等.桃内酯芳香物质合成相关的环氧化物水解酶候选基因的鉴别[J].江苏农业学报,2023,(01):178-186.[doi:doi:10.3969/j.issn.1000-4440.2023.01.021]
　ZHANG Yuan-yuan,LIU Wen-jing,ZHANG Bin-bin,et al.Identification of candidate epoxide hydrolase genes involved in the biosynthesis of lactone volatile compounds in peach (Prunus persica L.)[J].,2023,(01):178-186.[doi:doi:10.3969/j.issn.1000-4440.2023.01.021]
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桃内酯芳香物质合成相关的环氧化物水解酶候选基因的鉴别()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
178-186
栏目:
园艺
出版日期:
2023-02-28

文章信息/Info

Title:
Identification of candidate epoxide hydrolase genes involved in the biosynthesis of lactone volatile compounds in peach (Prunus persica L.)
作者:
张圆圆1; 刘文敬1; 2; 张斌斌1; 马瑞娟1; 俞明亮1
（1.江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，江苏南京210014；2.江苏大学食品与生物工程学院，江苏镇江212013）
Author(s):
ZHANG Yuan-yuan1; LIU Wen-jing1; 2; ZHANG Bin-bin1; MA Rui-juan1; YU Ming-liang1
(1.Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China；2.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)
关键词:
桃; 内酯芳香物质; 环氧化物水解酶
Keywords:
peach; lactones volatile compounds; epoxide hydrolases
分类号:
S662.1
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.021
文献标志码:
A
摘要:
环氧化物水解酶（EH）因其重要的生物学功能而在哺乳动物以及诸多植物中被广泛关注，更是果实典型“桃香”气味——内酯芳香物质生物合成的一个重要酶，但在桃等果实中的研究较少且鲜有该家族成员的系统报道或生物学功能的解析。为鉴别桃果实中与内酯芳香物质合成相关的环氧化物水解酶家族成员，本研究使用了同源序列比对和关键词搜索等方法，在桃中共筛选获得7个环氧化物水解酶家族成员。序列比对分析结果表明，这7个成员均有典型的α/β水解酶折叠结构和环氧化物水解酶保守的序列片段。进化树分析结果显示，桃的环氧化物水解酶成员与拟南芥、烟草等其他物种中已被鉴别的环氧化物水解酶成员的亲缘关系很近。基因表达分析结果显示，在桃的发育成熟进程中7个EH基因均在果实的中果皮表达，在果实发育前期均呈现较高的表达水平，转录模式包括3种类型。综合已报道的内酯芳香物质的生物合成通路以及前人指出的内酯芳香物质含量在果实成熟期开始显著增加的变化规律，推测桃环氧化物水解酶成员的表达量可能与内酯芳香物质的积累负相关。本研究结果为后续深入挖掘桃或者其他果实环氧化物水解酶家族成员的生物学功能尤其是参与内酯芳香物质合成的分子机理提供了参考。
Abstract:
Epoxide hydrolases （EHs） were extensively characterized in mammals and numerous plant species for their multiple biological functions, and acted as one important enzyme in the biosynthesis of lactones——a group of volatile compounds which endowed fruit a typical peach-like aroma. However, there were few reports on the family members or biological functions of epoxide hydrolases in peach or other fruits. To identify the EH members involved in the biosynthesis of lactone volatile compounds in peach fruit, homologous sequence alignment and keyword search were used in this study. A total of seven EH family members were screened in peach, and sequence alignment analysis showed that they all had the typical α/β-hydrolase fold structure and conserved sequences. Phylogenetic tree analysis showed that EH members in peach were closely related to the identified EH members in Arabidopsis, tobacco and other plant species. The results of gene expression analysis indicated that these EH genes were expressed in the mesocarp of peach fruit during the development and maturation, and showed a high expression level in the early stages of fruit development. The transcription patterns included three types. Based on the reported biosynthetic pathways of lactones and the fact that the content of lactones began to increase significantly at fruit ripening stages, it was speculated that the expression levels of the epoxide hydrolase genes may be negatively correlated with the contents of lactones volatile compounds. Overall, these results lay a foundation for further exploration of the biological functions of EH family members in peach or other fruits, especially the molecular mechanism involved in the biosynthesis of lactone volatile compounds.

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备注/Memo

备注/Memo:
收稿日期：2022-07-06基金项目：国家自然科学基金项目（32002020）；国家现代农业产业技术体系建设专项（CARS-30）；江苏现代农业（桃）产业技术体系项目[JATS(2020)379、JATS(2021)425]作者简介：张圆圆（1990-），女，河南平顶山人，博士，助理研究员，从事果实芳香品质研究。（E-mail）20190021@jaas.ac.cn
更新日期/Last Update: 2023-03-21