[1]高磊,李慧,洪奔,等.葡萄品种钟山红玉与钟山红杂交后代的SSR鉴定及葡萄果实性状遗传倾向分析[J].江苏农业学报,2023,(01):187-197.[doi:doi:10.3969/j.issn.1000-4440.2023.01.022]
 GAO Lei,LI Hui,HONG Ben,et al.SSR identification and genetic tendency analysis of fruit characters of hybrid offspring of Zhongshan Hongyu and Zhongshan Hong[J].,2023,(01):187-197.[doi:doi:10.3969/j.issn.1000-4440.2023.01.022]
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葡萄品种钟山红玉与钟山红杂交后代的SSR鉴定及葡萄果实性状遗传倾向分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
187-197
栏目:
园艺
出版日期:
2023-02-28

文章信息/Info

Title:
SSR identification and genetic tendency analysis of fruit characters of hybrid offspring of Zhongshan Hongyu and Zhongshan Hong
作者:
高磊李慧洪奔郑焕陶建敏
(南京农业大学园艺学院,江苏南京210095)
Author(s):
GAO LeiLI HuiHONG BenZHENG HuanTAO Jian-min
(College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
葡萄果实杂交F1代简单重复序列(SSR)分子标记遗传规律
Keywords:
grapefruithybrid F1 generationsimple sequence repeat (SSR) molecular markersgenetic law
分类号:
S663.103.3
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.022
文献标志码:
A
摘要:
以母本钟山红葡萄、父本钟山红玉葡萄及其50株杂交F1代植株为试验材料,通过简单重复序列(SSR)分子标记技术分析亲本与子代间的亲缘关系并绘制遗传关系聚类图,通过测定杂交F1代与父母本的果实基本品质及酚类物质含量等指标,以进一步了解钟山红玉、钟山红及其杂交F1代的遗传规律。结果表明,通过SSR分子标记技术检测,发现含有父母本特征性条带的杂交F1代占比不低于86%,杂交F1代与父母本的遗传相似系数为0.857~1.000,果实基本品质性状呈广泛分离的现象,符合数量性状的遗传特点。F1代果皮中的总花色苷含量呈低于双亲遗传的特点,果肉中总类黄酮含量、原花色素含量呈超高亲遗传的特点,但是果实颜色的遗传受到多基因的调控,还需要进一步研究。
Abstract:
Taking the female parent Zhongshan Hong, male parent Zhongshan Hongyu and their 50 hybrid F1 generations as experimental materials, the genetic relationship between parents and offspring was analyzed by simple sequence repeat (SSR) molecular marker technology, and the genetic similarity coefficient map was drawn. The genetic law of Zhongshan Hongyu, Zhongshan Hong and their hybrid offspring was further understood by measuring the basic fruit quality and phenolic content of F1 hybrids and their parents. The detection results obtained by SSR molecular marker technology showed that the proportion of F1 hybrids containing parental characteristic bands exceeded 86%, and the genetic similarity coefficient between F1 hybrids and parents ranged from 0.857 to 1.000. The basic quality indices of fruits were widely separated, which was in line with the genetic characteristics of quantitative traits. The content of total anthocyanins in pericarp of the F1 generation is lower than that of parental inheritance, and the content of total flavonoids and total proanthocyanidins in pulp is ultra-high. However, the inheritance of fruit color is regulated by multiple genes and needs further research.

参考文献/References:

[1]宁克远. 葡萄栽培技术[J]. 现代种业, 2009(5):42-43.
[2]IVANOVA N N,KHOMICH L M,PEROVA I B,et al. Grapefruit juice nutritional profile[J]. Voprosy Pitaniia, 2018,87(5): 85-94.
[3]王初田. 葡萄的营养价值及栽培技术[J]. 中国园艺文摘, 2012,28(9): 159-160.
[4]王心怡,赵节昌. 葡萄籽的营养功效及综合利用[J]. 农产品加工,2019(4):87-89.
[5]刘凤之. 中国葡萄栽培现状与发展趋势[J]. 落叶果树, 2017, 49(1): 1-4.
[6]罗国光. 中国葡萄栽培发展成就和需要关注的几个问题[C]//中国农学会. 第十三届全国葡萄学术研讨会论文集. 灃县:中国农学会,2007.
[7]张振文,刘延琳,惠竹梅. 中国葡萄栽培发展现状分析[C]//中国酿酒工业协会,中国食品工业协会.第二届国际葡萄与葡萄酒学术研讨会论文集. 杨凌:中国酿酒工业协会,中国食品工业协会,2001.
[8]沈甜,牛锐敏,黄小晶,等. 基于层次-关联度和主成分分析的无核鲜食葡萄品质评价[J]. 食品工业科技, 2021, 42(3): 53-60,67.
[9]赵玉山. 提高巨峰葡萄品质的措施[J]. 河北果树, 2013(4):49-50.
[10]陈奕霖. 天津产区酿酒葡萄品质与葡萄酒质量的研究[D]. 杨凌:西北农林科技大学,2013.
[11]李记明. 关于葡萄品质的评价指标[J]. 中外葡萄与葡萄酒, 1999(1): 56-59.
[12]刘政海,董志刚,李晓梅,等. ‘威代尔’与‘霞多丽’葡萄杂交F1代果实性状遗传倾向分析[J]. 果树学报, 2020, 37(8):1122-1131.
[13]李坤. 葡萄自交与杂交后代若干性状遗传特点及规律的研究[D]. 沈阳:沈阳农业大学,2003.
[14]刘政海,董志刚,李晓梅,等. 酿酒葡萄‘黑比诺’与‘马瑟兰’杂交后代果实性状遗传倾向分析[J]. 中国果树, 2020(6): 29-35.
[15]谭伟,李晓梅,董志刚,等. 毛欧杂种‘2-1-3’杂交F1代果实性状遗传倾向分析[J]. 果树学报,2018,35(12): 1444-1454.
[16]王勇,苏来曼·艾则孜,李玉玲,等. ‘火州黑玉’葡萄杂交后代果实性状遗传倾向分析[J]. 西北植物学报,2015,35(2):275-281.
[17]郭修武,郭印山,李轶晖,等.葡萄杂交后代主要经济性状的遗传倾向[J].果树学报,2004(4):319-323.
[18]万怡震,王治明,周存忠,等. 葡萄种子单宁含量的遗传研究[J]. 西北农业学报,2001,10(3): 110-111.
[19]宋润刚,郑永春,路文鹏,等. 山葡萄种内和种间杂交F1~F4代果实酸和糖含量的遗传分析[J]. 园艺学报,2007(4):813-822.
[20]WEI X M,SYKES S R,CLINGELEFFER P R. An investigation to estimate genetic parameters in CSIRO’s table grape breeding program. 2. Quality characteristics[J]. EupHytica, 2002,128(3): 343-351.
[21]谭伟,唐晓萍,董志刚,等. ‘赤霞珠’和‘梅露辄’不同营养系果实不同部位酚类物质含量的比较分析[J]. 中国农学通报, 2015,31(8): 229-234.
[22]TIAN S F. Changes of content and antioxidant activity of pHenolic compounds during gibberellin-induced development of seedless muscat grapevines[J]. Bruce Irving Reisch,2014,1046(1046): 539-548.
[23]WATERHOUSE A L, IGNELZI S,SHIRLEY J R. A comparison of methods for quantifying oligomeric proanthocyanidins from grape seed extracts[J]. American Journal of Enology & Viticulture, 2000,51(4): 383-389.
[24]MUNTANA N,PRASONG S. Study on total phenolic contents and their antioxidant activities of thai white,red and black rice bran extracts[J]. Pakistan Journal of Biological Sciences, 2010,13(4): 170-174.
[25]季晨飞,张明月,王继源,等. 葡萄雄性不育基因的初步定位[J]. 西北植物学报, 2014,34(11): 2165-2169.
[26]路文鹏,刘燕. 山葡萄杂交育种技术要点[J]. 特种经济动植物,2013,16(5):49-50.
[27]王玉环,姜学品,王桂华,等. 葡萄新品种巨玫瑰的选育[J]. 中国果树,2003(1): 4-7.
[28]胡福荣. “巨玫瑰”葡萄优质栽培技术[J]. 上海农业科技,2011(2): 55-56.
[29]郭艳芳,张娜,田淑芬,等. 以‘巨玫瑰’葡萄为母本的胚挽救育种研究[J]. 中外葡萄与葡萄酒,2019(2):46-49.
[30]项殿芳,李绍星,张孟宏,等. 鲜食葡萄新品种‘金田玫瑰’[J]. 园艺学报,2008(6):926.
[31]郭修武,郭印山,李轶晖,等. 葡萄主要性状遗传规律研究[C]//中国园艺学会. 中国园艺学会青年学术讨论会论文集. 杨凌:中国园艺学会,2002.
[32]郭权,郭印山,郭修武. 葡萄‘红地球’与‘双优’杂交后代果实糖酸的遗传规律[J]. 分子植物育种, 2021,19(10): 3424-3431.
[33]刘振中,樊红科,高华,等. 苹果杂交后代果实性状遗传变异分析[J]. 北方园艺, 2012(5): 5-8.
[34]路文鹏,宋润刚,李晓红,等. 山葡萄种内杂交后代浆果色素、单宁和总酸含量的遗传分析[J]. 吉林农业大学学报, 2003, 25(6): 629-633.
[35]SUNIL L,SHETTY N P. Biosynthesis and regulation of anthocyanin pathway genes[J]. Applied Microbiology and Biotechnology, 2022,106(5/6):1783-1798.
[36]李坤,郭修武,谢洪刚,等.葡萄自交与杂交后代果皮色素含量的遗传[J]. 果树学报, 2004,21(5): 406-408.
[37]邱炳发,梁馨元,王建忠,等.大花序桉基因组SSR的分布特征及序列分析[J].南方农业学报,2021,52(10):2744-2750.
[38]王掌军,许娜丽,王新华,等.宁春4号与河东乌麦杂交F2∶5家系的籽粒品质性状及其QTL分析[J].南方农业学报,2021,52(9):2391-2403.
[39]高阳,侯非凡,熊雄,等.基于候选基因开发gSSR标记加密黄花菜和萱草种间遗传图谱[J].江苏农业学报,2021,37(1):139-149.
[40]罗登杰,万瑶,覃雪梅,等.水稻细菌性条斑病抗性基因bls2 SSR分子标记开发[J].南方农业学报,2021,52(5):1167-1173.

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备注/Memo

备注/Memo:
收稿日期:2022-03-20基金项目:国家自然科学基金项目(31901975、31972384);国家现代农业产业技术体系项目(CARS-29);江苏农业产业技术体系项目[JATS(2021)450];江苏省农业重大新品种创制项目(PZCZ201723);国家重点研发计划项目(2020YFD1000204)作者简介:高磊(1998-),男,山西大同人,硕士研究生,主要从事果树生理与分子生物学研究。(E-mail)15909824016@163.com通讯作者:陶建敏,(E-mail)taojianmin@njau.edu.cn
更新日期/Last Update: 2023-03-21