[1]朱凌丽,徐建,姚协丰,等.厚皮甜瓜种质蔓枯病抗性评价与遗传多样性分析[J].江苏农业学报,2021,(02):454-464.[doi:doi:10.3969/j.issn.1000-4440.2021.02.023]
　ZHU Ling-li,XU Jian,YAO Xie-feng,et al.Identification of gummy stem blight resistance and analysis of genetic diversity in muskmelon germplasms[J].,2021,(02):454-464.[doi:doi:10.3969/j.issn.1000-4440.2021.02.023]
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厚皮甜瓜种质蔓枯病抗性评价与遗传多样性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
454-464
栏目:
园艺
出版日期:
2021-04-30

文章信息/Info

Title:
Identification of gummy stem blight resistance and analysis of genetic diversity in muskmelon germplasms
作者:
朱凌丽1; 2; 徐建2; 姚协丰2; 徐锦华2; 李苹芳2; 柳李旺1; 羊杏平2
（1.南京农业大学园艺学院，江苏南京210095；2.江苏省农业科学院蔬菜研究所，江苏南京210014）
Author(s):
ZHU Ling-li1; 2; XU Jian2; YAO Xie-feng2; XU Jin-hua2; LI Ping-fang2; LIU Li-wang1; YANG Xing-ping2
（1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Vegetable, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China）
关键词:
厚皮甜瓜; 种质资源; 蔓枯病; 抗性评价; 遗传多样性
Keywords:
muskmelon; germplasm resources; gummy stem blight; resistance evaluation; genetic diversity
分类号:
S652
DOI:
doi:10.3969/j.issn.1000-4440.2021.02.023
文献标志码:
A
摘要:
采用苗期蔓枯病接种和单核苷酸多态性（SNP）基因型分析对120份厚皮甜瓜高代自交系进行抗性评价与遗传多样性研究。结果显示：光皮类型厚皮甜瓜中高抗、中抗种质资源占比达11.11%，其抗性水平结构优于网纹甜瓜和哈密瓜。统计分析结果显示，21个SNP位点的PIC值为0.050～0.478，其中12个中度多态SNP位点的PIC值平均为0.37，表明甜瓜种质的多态性中等。群体结构与聚类分析结果表明，124份甜瓜种质资源可分成5大类群，各类群具有一定的独立性，且类群间存在明显的差异，遗传多样性丰富。
Abstract:
The gummy stem blight (GSB) resistance evaluation and genetic diversity study of 120 high generation inbred lines of muskmelons were conducted via inoculation at seedling stage and single nucleotide polymorphism (SNP) genotype analysis. The results showed that, the proportion of germplasm resources of smoothbark muskmelons with high and medium resistance to GSB was up to 11.11%, and the structure of resistance level was better than that of netted melon and Hami melon. The statistical analysis results showed that, the polymorphism information content (PIC) value of 21 SNP loci ranged from 0.050 to 0.478, and the average PIC value of 12 moderate polymorphic SNP loci was 0.37, indicating that the polymorphism of the melon germplasms was moderate. Results of population structure and clustering analysis showed that, the 124 melon germplasms could be classified into five major groups, and each group showed certain independence. Furthermore, there were obvious differences between different groups and the genetic diversity was abundant.

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备注/Memo

备注/Memo:
收稿日期：2020-07-02基金项目：国家西甜瓜产业技术体系项目(CARS-26-08)；江苏省农业三新工程项目[SXGC（2017）088]；徐州市重点研发计划项目（KC18119）作者简介：朱凌丽（1981-），女，江苏盐城人，硕士，助理研究员，主要从事甜瓜新品种选育工作。（E-mail）sweetmelon2050@foxmail.com通讯作者：柳李旺，（E-mail）nauliulw@njau.edu.cn；羊杏平，（E-mail）xingping@jaas.ac.cn
更新日期/Last Update: 2021-05-10