[1]蔡瑾,杨继书,翟文玲,等.长江中下游麦区小麦低多酚氧化酶活性种质的初步鉴定[J].江苏农业学报,2021,(03):545-554.[doi:doi:10.3969/j.issn.1000-4440.2021.03.001]
 CAI Jin,YANG Ji-shu,ZHAI Wen-ling,et al.Preliminary identification of wheat germplasm with low polyphenol oxidase activity in the wheat region of the middle and lower reaches of Yangtze River valley[J].,2021,(03):545-554.[doi:doi:10.3969/j.issn.1000-4440.2021.03.001]
点击复制

长江中下游麦区小麦低多酚氧化酶活性种质的初步鉴定()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年03期
页码:
545-554
栏目:
遗传育种·生理生化
出版日期:
2021-06-30

文章信息/Info

Title:
Preliminary identification of wheat germplasm with low polyphenol oxidase activity in the wheat region of the middle and lower reaches of Yangtze River valley
作者:
蔡瑾杨继书翟文玲付必胜张巧凤吴纪中
(江苏省农业科学院种质资源与生物技术研究所,江苏南京210014)
Author(s):
CAI JinYANG Ji-shuZHAI Wen-lingFU Bi-shengZHANG Qiao-fengWU Ji-zhong
(Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
小麦多酚氧化酶活性种质资源
Keywords:
wheatpolyphenol oxidase activitygermplasm
分类号:
S512.102.4
DOI:
doi:10.3969/j.issn.1000-4440.2021.03.001
文献标志码:
A
摘要:
选取145份长江中下游麦区小麦种质以及1份美国种质和1份意大利种质进行籽粒多酚氧化酶 (PPO)活性分析及2个主效基因等位研究。结果表明长江中下游麦区种质间籽粒PPO活性差异明显,具有很大的遗传改良潜力;运用2个PPO活性主效基因的功能标记检测上述147份种质,发现4种基因型PPO活性均值大小顺序为:Ppo-D1aPpo-D1aPpo-A1bPpo-A1b (L1L1L2L2)
Abstract:
145 wheat germplasms of the wheat region in the middle and lower reaches of the Yangtze River, an American germplasm and an Italian germplasm were selected for the analysis of grain polyphenol oxidase (PPO) activity and the study of two allelic variation of major genes. The results showed that, the grain PPO activities between different wheat germplasms of the wheat region in the middle and lower reaches of the Yangtze River varied obviously and had great potential in genetic improvement. After detecting the above 147 germplasms by two functional markers of major genes related to PPO activity, average PPO activities of four genotypes showed the following order: Ppo-D1aPpo-D1aPpo-A1bPpo-A1b (L1L1L2L2)

参考文献/References:

[1]KRUGER J E, HATCHER D W, DEPAUW R A. Whole seed assay for polyphenol oxidase in Canadian prairie spring wheats and its usefulness as a measure of noodle darkening [J]. Cereal Chemistry, 1994, 71: 324-326.
[2]MAYER A M, HAREL E. Polyphenol oxidases in plants [J]. Phytochemistry, 1979, 18:193-215.
[3]TARANTO F, PASQUALONE A, MANGINI G, et al. Polyphenol oxidases in crops: biochemical, physiological and genetic aspects [J]. International Journal of Molecular Science, 2017, 18: 377.
[4]KRUGER J E. Effects of flour refinement on raw Cantonese noodle color and texture [J]. Cereal Chemistry, 1994, 71:177-182.
[5]MARTIN J M, BERG J E, HOFER P, et al. Allelic variation of polyphenol oxidase genes impacts on Chinese raw noodle color [J]. Journal of Cereal Science, 2011, 54:387-394.
[6]BEECHER B S, CARTER A H, SEE D R. Genetic mapping of a new family of seed-expressed polyphenol oxidase genes in wheat (Triticum aestivumL.) [J]. Theoretical and Applied Genetics, 2012, 124:1463-1473.
[7]葛秀秀,张立平,何中虎,等. 冬小麦PPO活性的主基因+多基因混合遗传分析 [J]. 作物学报, 2004, 30(1):18-20.
[8]张立平,葛秀秀,何中虎,等. 普通小麦多酚氧化酶活性的QTL分析 [J]. 作物学报, 2005, 25(1):7-10.
[9]RAMAN R, RAMAN H, JOHNSTONE K, et al. Genetic and in silico comparative mapping of the polyphenol oxidase gene in bread wheat (Triticum aestivum L.) [J]. Functional & Integrative Genomics, 2005,5:185-200.
[10]BEECHER B S, SKINNER D. Molecular cloning and expression analysis of multiple polyphenol oxidase genes in developing wheat (Triticum aestivum) kernels [J]. Journal of Cereal Science, 2011, 53:371-378.
[11]SUN D J, HE Z H, XIA X C, et al. A novel STS marker for polyphenol oxidase activity in bread wheat [J]. Molecular Breeding, 2005, 16:209-218.
[12]NILTHONG S, GRAYBOSCH R A, BAENZIGER P S. Enzyme activity in wheat breeding lines derived from matings of low polyphenol oxidase parents [J]. Euphytica, 2013, 190:65-73.
[13]DEMEKE T, MORRIS C F. Wheat polyphenol oxidase: distribution and genetic mapping in three inbred line populations [J]. Crop Science, 2001, 41:1750-1757.
[14]ANDERSON J V, MORRIS C F. An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity [J]. Crop Science, 2001, 41:1697-1705.
[15]WATANABE N, TAKEUCHI A, NAKAYAMA A. Inheritance and chromosomal location of the homoelogous genes affecting phenol colour of kernels in durum wheat [J]. Euphytica, 2004, 139:87-93.
[16]ZHAI S N, HE Z H, WEN W E, et al. Genome-wide linkage mapping of flour color-related traits and polyphenol oxidase activity in common wheat [J]. Theoretical and Applied Genetics, 2016, 129(2):377-394.
[17]HE X Y, HE Z H, ZHANG L P, et al. Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat [J]. Theoretical and Applied Genetics, 2007, 115:47-58.
[18]王小波,马传喜,何克勤,等. 小麦2D染色体上多酚氧化酶(PPO)基因STS标记的开发与应用 [J]. 中国农业科学, 2008, 41(6):1583-1590.
[19]WANG X B, MA C X, SI H Q, et al. Gene markers for grain polyphenol oxidase activity in common wheat [J]. Molecular Breeding, 2009, 23(1):163-170.
[20]周志良,司红起,闫小燕,等. 小麦籽粒2A染色体PPO基因新分子标记的开发与应用[J]. 分子植物育种, 2011, 9(1):81-86.
[21]常成. 小麦及近缘种属籽粒硬度、多酚氧化酶性状的分子机理研究[D]. 北京:中国农业大学. 2005.
[22]SUN Y, HE Z, MA W, et al. Alternative splicing in the coding region of Ppo-A1 directly influences the polyphenol oxidase activity in common wheat (Triticum aestivum L.) [J]. Functional & Integrative Genomics, 2011, 11: 85-93.
[23]SI H, ZHOU Z, WANG X, et al. A novel molecular marker for the polyphenol oxidase gene located on chromosome 2B in common wheat [J]. Molecular Breeding, 2012, 30(3):1371-1378.
[24]TARANTO F, MANGINI G, PASQUALONE A, et al. Mapping and allelic variation of Ppo-B1 and Ppo-B2 gene-related polyphenol oxidase activity in durum wheat [J]. Molecular Breeding, 2015, 35:80
[25]PARK W J, SHELTON D R, PETERSON C J, et al. Variation in polyphenol oxidase activity and quality characteristics among hard white wheat and hard red winter wheat samples[J]. Cereal Chem, 1997, 74:7-11.
[26]葛秀秀,何中虎,杨金,等. 我国小麦品种多酚氧化酶活性的遗传变异及其与品质的相关分析 [J]. 作物学报, 2003, 29(4): 481-485.
[27]肖永贵,何心尧,刘建军,等. 中国冬小麦品种多酚氧化酶活性基因等位变异检测及其分布规律研究 [J]. 中国农业科学, 2008, 41(4): 954-960.
[28]BAIK B K, CZUEHJOWSKA Z, POMERANZ Y. Comparison of polyphenol oxidase activities in wheats and flours from Australian and U.S. cultivars [J]. Journal of Cereal Science, 1994, 19: 291- 296.
[29]马传喜,王晓波,司红起,等. 小麦多酚氧化酶活性的品种间差异及其遗传分析研究进展 [J]. 安徽农业大学学报, 2007, 34(3): 305-310.
[30]何克勤,马传喜,王晓波,等. 小麦低多酚氧化酶活性品种资源的筛选 [J]. 麦类作物学报, 2007, 27(4): 603-606.
[31]陈泠,高春保,朱展望,等. 小麦黄色素含量与多酚氧化酶活性相关基因的分子标记检测及分布差异 [J]. 湖北农业科学, 2017, 56(24): 4893-4898.
[32]SOUZA E J, GUTTIERI M J, UDALL J A. Registration of ‘IDO580’ spring wheat germplasm [J]. Crop Science, 2005,45: 429-430.
[33]ONTO S. Genetics of polyphenol oxidase (PPO) activity in wheat (Triticum aestivumL.) [D]. Nebraska, USA: University of Nebraska, 2011.
[34]SAGHAI-MAROOF M A, SOLIMAN K M, JORGENSEN RA, et al. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics [J]. Proceeding of National Academy Science, USA, 1984, 81:8014-8018.

相似文献/References:

[1]伍 宏,朱昌华,夏 凯,等.叶面喷施激动素对小麦品种济麦22品质的影响[J].江苏农业学报,2016,(02):299.[doi:10.3969/j.issn.1000-4440.2016.02.010]
 WU Hong,ZHU Chang-hua,XIA Kai,et al.Effect of foliar application of kinetin on quality of Triticum aestivum L. Jimai 22[J].,2016,(03):299.[doi:10.3969/j.issn.1000-4440.2016.02.010]
[2]蒋正宁,别同德,赵仁惠,等.受条锈菌诱导的小麦丝氨酸苏氨酸激酶基因TaS/TK的克隆与表达[J].江苏农业学报,2016,(05):980.[doi:10.3969/j.issn.1000-4440.2016.05.004]
 JIANG Zheng-ning,BIE Tong-de,ZHAO Ren-hui,et al.Cloning and expression analysis of a Serine/Threonine protein kinase gene TaS/TK in wheat in response to stripe rust fungal infection[J].,2016,(03):980.[doi:10.3969/j.issn.1000-4440.2016.05.004]
[3]丁彬彬,张旭,吴磊,等.小麦3B 短臂染色体抗赤霉病主效 QTL 区域候选基因的表达[J].江苏农业学报,2017,(01):6.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
 DING Bin-bin,ZHANG Xu,WU Lei,et al.Expression of candidate genes on the region of a major QTL for the resistance to Fusarium head blight on the short arm of chromosome 3B in wheat[J].,2017,(03):6.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
[4]周淼平,姚金保,张鹏,等.小麦幼苗纹枯病抗性评价新方法[J].江苏农业学报,2017,(01):61.[doi:10.3969/j.issn.1000-4440.2017.01.010 ]
 ZHOU Miao-ping,YAO Jin-bao,ZHANG Peng,et al.New method for the resistance evaluation of wheat sharp eyespot in seedling[J].,2017,(03):61.[doi:10.3969/j.issn.1000-4440.2017.01.010 ]
[5]吴磊,姜朋,张瑜,等.苏麦3号小麦穗部病毒诱导的基因沉默(VIGS)体系的建立及验证[J].江苏农业学报,2017,(02):248.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
 WU Lei,JIANG Peng,ZHANG Yu,et al.Construction and validation of virus-induced gene silencing(VIGS) system in spike of wheat variety Sumai 3[J].,2017,(03):248.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
[6]邵继锋,陈荣府,董晓英,等.利用分根技术研究小麦铝磷交互作用[J].江苏农业学报,2016,(01):78.[doi:10.3969/j.issn.1000-4440.2016.01.012 ]
 SHAO Ji-feng,CHEN Rong-fu,DONG Xiao-ying,et al.Aluminum-phosphorus interaction in wheat grown in a split-root device[J].,2016,(03):78.[doi:10.3969/j.issn.1000-4440.2016.01.012 ]
[7]叶景秀.小麦籽粒蛋白质双向电泳体系的优化[J].江苏农业学报,2015,(05):957.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
 YE Jing-xiu.Optimization of two-dimensional electrophresis system for grain protein in spring wheat[J].,2015,(03):957.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
[8]郑舒文,徐其隆,邹华文.脱落酸对涝渍胁迫下小麦产量的影响[J].江苏农业学报,2015,(05):967.[doi:doi:10.3969/j.issn.1000-4440.2015.05.004]
 ZHENG Shu-wen,XU Qi-long,ZOU Hua-wen.Yield of waterlogged wheat in response to ABA application[J].,2015,(03):967.[doi:doi:10.3969/j.issn.1000-4440.2015.05.004]
[9]张玉萍,马占鸿.不同施氮量下小麦遥感估产模型构建[J].江苏农业学报,2015,(06):1325.[doi:doi:10.3969/j.issn.1000-4440.2015.06.020]
 ZHANG Yu-ping,MA Zhan-hong.Yield estimation model of wheat based on remote sensing data under different nitrogen supply conditions[J].,2015,(03):1325.[doi:doi:10.3969/j.issn.1000-4440.2015.06.020]
[10]张卓亚,王晓琳,许晓明,等.腐植酸对小麦扬花期水分利用效率及灌浆进程的影响[J].江苏农业学报,2015,(04):725.[doi:10.3969/j.issn.1000-4440.2015.04.003]
 ZHANG Zhuo-ya,WANG Xiao-ling,XU Xiao-ming,et al.Effect of humic acid on water use efficiency and grouting process of wheat at flowering[J].,2015,(03):725.[doi:10.3969/j.issn.1000-4440.2015.04.003]

备注/Memo

备注/Memo:
收稿日期:2020-09-04基金项目:江苏省自然科学基金项目(BK20170596);江苏省农业科技自主创新基金项目[CX(18) 1001]作者简介:蔡瑾(1985-),女,江苏南京人,博士,助理研究员,研究方向为小麦种质资源等。(E-mail)caijin@jaas.ac.cn通讯作者:吴纪中,(E-mail)wujz@jaas.ac.cn
更新日期/Last Update: 2021-07-05