[1]叶景秀.小麦籽粒蛋白质双向电泳体系的优化[J].江苏农业学报,2015,(05):957-961.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
 YE Jing-xiu.Optimization of two-dimensional electrophresis system for grain protein in spring wheat[J].,2015,(05):957-961.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
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小麦籽粒蛋白质双向电泳体系的优化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
957-961
栏目:
遗传育种·生理生化
出版日期:
2015-10-31

文章信息/Info

Title:
Optimization of two-dimensional electrophresis system for grain protein in spring wheat
作者:
叶景秀
(青海省农林科学院,青海西宁810016)
Author(s):
YE Jing-xiu
(Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016, China)
关键词:
小麦籽粒蛋白质双向电泳
Keywords:
wheatgrain proteintwo-dimensional electrophoresis
分类号:
S512.1+2
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.002
文献标志码:
A
摘要:
为建立一套适合于小麦籽粒蛋白质双向电泳的技术体系,以青海省农林科学院自育春小麦品种青春38籽粒为材料,对比了2种在植物组织中常用的蛋白质提取方法,对双向电泳过程中胶条pH值、蛋白质上样量和分离胶浓度等方面进行了优化。结果表明:与用酚提取法相比采用TCA/丙酮沉淀法提取的蛋白质所得的双向电泳图谱质量较好,蛋白质经裂解后在17 cm、pH 5~8的IPG胶条、上样量380 μg和分离胶浓度12%的条件下得到了清晰、蛋白质点丰富的双向电泳图谱。
Abstract:
To develop a system for two-dimensional electrophresis(2-DE) of grain protein extracted from spring wheat, two protein extraction methods commonly used for plant tissues were compared. The pH range, the loading quantity of sample and the gel concentrations were optimized in the 2-DE system for the grains from Qingchun 38. The samples prepared by TCA/acetone precipitation yielded better quality 2-DE map than by phenol extraction method. The condition of 17-cm IPG with pH 5-8, 380 μg loading quantity of protein and 12% SDS-PAGE gel gave rich protein spots in the 2-DE map.〖JP〗

参考文献/References:

[1]郭明明, 赵广才, 郭文善, 等.播期对不同筋力型小麦旗叶光合及籽粒灌浆特性的影响[J].麦类作物学报, 2015,35(2): 192-197. 
[2]吴晓丽, 汤永禄, 李朝苏, 等.不同生育时期渍水对冬小麦旗叶叶绿素荧光及籽粒灌浆特性的影响[J]. 中国生态农业学报, 2015,23(3): 309-318. 
[3]刘希伟, 张敏, 姚凤娟, 等.花后不同强度遮光对糯小麦和非糯小麦干物质积累和产量的影响[J].麦类作物学报, 2015,35(4): 521-527.
[4]石培春, 李英枫, 韩璐, 等.不同品质类型小麦籽粒淀粉含量积累的动态差异[J]. 石河子大学学报:自然科学版, 2012,4(30): 417-421. 
[5]TISCHNER R. Nitrate uptake and reduction in higher and lower plants[J]. Plant Cell and Environment, 2002,23: 1015-1024.
[6]LIU C W, CHANG T S, HSU Y K, et al. Comparative proteomic analysis of early salt stress responsive proteins in roots and leaves of rice[J]. Proteomics, 2014,14:1759-1775.
[7]PENG Z Y, WANG M C, LI F, et al. A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat[J]. Molecular Cellular Proteomics, 2009,8(12): 2676-2686.
[8]GALLARDO K, JOB C, GROOT S P C, et al. Proteomics of Arabidopsis seed germination:a comparative study of wild-type and gibberellin-deficient seeds[J].Plant Physiology, 2002,129:823-837.〖JP〗
[9]HOUSTON N L, HAJDUCH M, THELEN J J. Quantitative proteomics ofseed filling in castor: comparison with soybean and rapeseed revealsdifferences between photosynthetic and nonphotosyntheticseed metabolism[J]. Plant Physiol, 2009,151:857-868.
[10]ROCCOA M, CORRADOB G, ARENAC S, et al. The expressionof tomato prosystemin gene in tobacco plants highly affects hostproteomic repertoire[J]. Joural Proteomics, 2008,71: 176-185.〖JP〗
[11]王慧娜,高建华,张淑英,等.不同方法提取的小麦籽粒蛋白双向电泳凝胶分离效果的分析比较[J].分子植物育种, 2014,12(4): 788-795. 
[12]陈蕊红,张改生,刘卫,等.小麦花药蛋白质组双向电泳技术体系的优化[J]. 核农学报, 2008,22(4):404-409. 
[13]水燕,管政兵,赵朝阳,等.克氏原螯虾卵巢发育Ⅳ期与IV期的比较蛋白质组学研究[J].江苏农业科学,2013,41(3):179-183.
[14]增广娟,李春敏,张新忠,等.适于双向电泳分析的苹果叶片蛋白质提取方法[J].色谱, 2009,27(4):484-488. 
[15]石海波,王云生,冯勇,等.玉米籽粒蛋白质双向电泳技术体系的优化[J].华北农学报,2015,30(1): 171-176.
[16]李奇松,陈军,林世圣,等.水稻籽粒蛋白双向电泳条件的优化及其蛋白组学方法的比较[J]. 作物学报, 2012,38(5): 921-927. 
[17]王娜,谢惠民,王川.冬小麦叶片抗旱蛋白质组双向电泳技术体系的优化[J]. 麦类作物学报,2011,31(3): 443-449.〖ZK)〗〖FL)〗

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备注/Memo

备注/Memo:
收稿日期:2015-06-16 基金项目:青海省农林科学院创新基金重大专项项目(2014-NKY-203);青海省(应用)基础研究计划项目(2013-Z-721) 作者简介:叶景秀(1979-),女,山东临沂人,硕士,助理研究员,现主要从事蛋白质组学和小麦育种方面的研究工作。(E-mail)sdyejingxiu@163.com
更新日期/Last Update: 2015-10-31