[1]吴磊,姜朋,张瑜,等.苏麦3号小麦穗部病毒诱导的基因沉默（VIGS）体系的建立及验证[J].江苏农业学报,2017,(02):248-252.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
　WU Lei,JIANG Peng,ZHANG Yu,et al.Construction and validation of virus-induced gene silencing(VIGS) system in spike of wheat variety Sumai 3[J].,2017,(02):248-252.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
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苏麦3号小麦穗部病毒诱导的基因沉默（VIGS）体系的建立及验证()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年02期
页码:
248-252
栏目:
遗传育种·生理生化
出版日期:
2017-04-30

文章信息/Info

Title:
Construction and validation of virus-induced gene silencing(VIGS) system in spike of wheat variety Sumai 3
作者:
吴磊; 姜朋; 张瑜; 马鸿翔; 张旭
江苏省农业科学院农业生物技术研究所/江苏省农业生物学重点实验室，江苏南京210014
Author(s):
WU Lei; JIANG Peng; ZHANG Yu; MA Hong-xiang; ZHANG Xu
(Institute of Agricultural Biotechnology,Jiangsu Academy of Agricultural Sciences/Provincial Key Laboratory of Agrobiology, Nanjing 210014, China)
关键词:
小麦; 赤霉病抗性; 病毒诱导的基因沉默（VIGS）
Keywords:
wheat; resistance to FHB; virus-induced gene silencing
分类号:
S512.103.2
DOI:
doi:10.3969/j.issn.1000-4440.2017.02.002
文献标志码:
A
摘要:
为了探讨利用病毒诱导的基因沉默（VIGS）体系在小麦穗部进行基因沉默的可行性，用大麦条纹花叶病毒诱导苏麦3号小麦穗部PDS基因的转录后沉默，再接种禾谷镰刀菌进行抗性鉴定。结果表明，与接种空载BSMV∶00的对照相比，接种BSMV∶PDS的小麦穗中PDS基因表达量下调了77% (P<0.05)。接种BSMV∶00的小麦穗受到赤霉病菌侵染后，穗部平均病小穗数和DON毒素含量分别为2.75个和4.55 μg/g，与接种无菌水对照无显著差异(P>0.05)。说明在苏麦3号穗部VIGS系统中，通过接种病毒能使靶标基因沉默；接种赤霉病菌后，苏麦3号病小穗数和DON毒素含量不受接种病毒的影响，对赤霉病菌的抗性保持不变，建立的VIGS体系可以应用于小麦赤霉病抗性候选基因的功能分析。
Abstract:
Virus-induced gene silencing (VIGS) was performed in the spikes of wheat variety Sumai3 by constructing VIGS vectors and silencing PDS, in order to explore the feasibility of gene silencing by VIGS system in spikes of wheat. The results showed that the expression level of PDS in the spikes inoculated by barley stripe mosaic virus(BSMV)∶PDS was reduced by 77% in comparison with that by BSMV∶00 (P<0.05). After infection with Fusarium asiaticum, the average number of diseased spikelets and DON content were 2.75 and 4.55 μg/g，respectively， showing no significant difference with the control group (P>0.05). The results suggested that the VIGS system for spike enabled the target gene silencing. The number of diseased spikelets and accumulation of DON toxin in Sumai 3 were not affected by the virus inoculation, and the FHB resistance remained unchanged, indicating that the VIGS system for Sumai3 could be applied to functional analysis of candidate genes for wheat FHB resistance.

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备注/Memo

备注/Memo:
收稿日期：2016-07-19基金项目：江苏省农业生物学重点实验室项目(49114042014Z012)；国家公益性行业专项(201303016)；江苏省重点研发项目(BE2013439、BE2015352)作者简介：吴磊(1986-), 男，湖北仙桃人，博士，助理研究员，研究方向为小麦抗赤霉病的分子机理和遗传育种。(Tel) 025-84390293; (E-mail) wulei686@sina.com通讯作者：张旭，(E-mail)xuzhang@jaas.ac.cn
更新日期/Last Update: 2017-05-02