[1]吴海霞,田志芳.银杏果实(白果)多糖提取工艺优化及其抑菌活性分析[J].江苏农业学报,2020,(06):1551-1558.[doi:doi:10.3969/j.issn.1000-4440.2020.06.027]
　WU Hai-xia,TIAN Zhi-fang.Optimization of extraction technology of polysaccharides from Ginkgo biloba L. fruit(Ginkgo biloba seed) and antibacterial activity[J].,2020,(06):1551-1558.[doi:doi:10.3969/j.issn.1000-4440.2020.06.027]
点击复制

银杏果实(白果)多糖提取工艺优化及其抑菌活性分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1551-1558
栏目:
加工贮藏·质量安全
出版日期:
2020-12-31

文章信息/Info

Title:
Optimization of extraction technology of polysaccharides from Ginkgo biloba L. fruit(Ginkgo biloba seed) and antibacterial activity
作者:
吴海霞1; 田志芳2
(1.运城学院生命科学系/特色农产品加工山西省重点实验室，山西运城044000；2.山西省农业科学院农产品加工研究所，山西太原030031)
Author(s):
WU Hai-xia1; TIAN Zhi-fang2
(1.Department of Life Science, Yuncheng University/Shanxi Key Laboratory of Characteristic Agro-Products Processing, Yuncheng 044000, China;2.Institute of Agro-Products Processing, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China)
关键词:
银杏果实(白果); 多糖; 响应面分析; 抑菌活性
Keywords:
Ginkgo biloba L. fruit(Ginkgo biloba seed); polysaccharide; response surface method; antibacterial activity
分类号:
S792.950.1
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.027
文献标志码:
A
摘要:
以银杏(Ginkgo biloba L.)果实(白果)为原料，利用响应面分析法优化白果多糖的提取工艺，并分析白果多糖的抑菌活性。以多糖得率为指标，通过单因素试验及响应面分析，探讨液料比、提取温度及提取时间等因素对白果多糖得率的影响，从而建立白果多糖的提取工艺模型，得出其最佳提取工艺条件；结合牛津杯法及二倍稀释法，分析白果多糖的抑菌活性及最小抑菌质量浓度。结果表明，白果多糖的最佳提取工艺条件如下：液料比45 ml∶1 g，95 ℃浸提3.0 h，在该条件下多糖得率为19.67%。抑菌试验结果表明，白果多糖对细菌有一定的抑制活性，对枯草芽孢杆菌(Bacillus subtilis)的抑制活性最强，最小抑菌质量浓度为2.50 mg/ml；白果多糖对大肠杆菌(Escherichia coli)的抑制活性也较强，当白果多糖质量浓度为15.00 mg/ml时，抑菌圈直径约为2.25 cm；白果多糖对真菌几乎无抑制活性，因而不具有广谱抑菌活性。
Abstract:
Ginkgo biloba seed was used as raw material, the conditions of extracting polysaccharides were optimized based on response surface method (RSM), and the antibacterial activity was studied. The yield of polysaccharide was used as the index, single factor tests and RSM were performed to study the effects of liquid-to-solid ratio, extraction temperature and extraction time on polysaccharide yield. The mathematical model of polysaccharides extraction was established, and the optimum extraction conditons were obtained. The antibacterial activity and minimum inhibitory concentration of polysaccharides were analyzed by Oxford cup method and double dilution method. The results showed that the optimum extraction conditons were as follows: liquid-to-solid ratio 45∶1(ml/g), extraction temperature 95 ℃ and extraction time 3.0 h. Under the optimum extraction conditions, the yield of polysaccharide was 19.67%. The results of antibacterial test indicated that polysaccharide from Ginkgo biloba seed showed the strongest antimicrobial activity against Bacillus subtilis, and the minimum inhibitory concentration was 2.50 mg/ml. The polysaccharide from Ginkgo biloba seed also had strong antibacterial activity against Escherichia coli. When the mass concentration of polysaccharide was 15.00 mg/ml, the antibacterial diameter was 2.25 cm. But the polysaccharide has no inhibitory activity against fungi and has no broad-spectrum antimicrobial activity.

参考文献/References:

[1]曹福亮. 中国银杏志[M]. 北京: 中国林业出版社, 2007.
[2]杨剑婷,吴彩娥. 白果致过敏成分及其致敏机理研究进展[J]. 食品科技, 2009, 34(6): 282-286.
[3]吴海霞,吴彩娥,刘金达,等. 银杏种仁酚酸的纯化、鉴定及其抑菌活性分析[J]. 中国食品学报, 2015, 15(3): 207-215.
[4]NAKAMURA A, TAKAHASHI T, YOSHIDA R, et al. Emulsifying properties of soybean soluble polysaccharide[J]. Food Hydrocolloids, 2004, 18(5): 795-803.
[5]YE C L, HUANG Q. Extraction of polysaccharides from herbal Scutellaria barbata D. Don (Ban-Zhi-Lian) and their antioxidant activity[J]. Carbohydrate Polymers, 2012, 89(4): 1131-1137.
[6]TIAN Y T, ZENG H L, XU Z B, et al. Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom (Agaricus bisporus)[J]. Carbohydrate Polymers, 2012, 88(2): 522-529.
[7]ZENG W C, ZHANG Z, GAO H, et al. Characterization of antioxidant polysaccharides from Auricularia auricular using microwave-assisted extraction[J]. Carbohydrate Polymers, 2012, 89(2): 694-700.
[8]陈群,刘天骄. 银杏叶多糖的抗肿瘤和免疫调节作用[J].中药药理与临床, 2003, 19(5): 18-19.
[9]许爱华,陈华圣,孙步蟾,等. 银杏外种皮多糖对人胃癌细胞凋亡及其凋亡诱导基因表达的影响[J].中药药理与临床, 2003, 19(3): 11-13.
[10]费文勇,彭爱军,王爱萍,等. 银杏外种皮多糖拮抗D-半乳糖致小鼠衰老作用的实验研究[J].辽宁中医学院学报，2004，6(1): 56-57.
[11]靳菊情,丁东宁,边晓丽,等. 银杏叶多糖的化学及清除羟自由基作用[J].西安医科大学学报， 2000，21(5): 417-419.
[12]姚以才,耿中华,王乃馨,等. 芦根多糖的超声辅助提取及其抗菌活性[J]. 食品科学, 2011, 32(14): 147-151.
[13]JIANG S J, MA Y H, YAN D Z. Antioxidant and antimicrobial properties of water soluble polysaccharide from Arachis hypogaea seeds[J]. Journal of Food Science & Technology. doi: 10.1007/s13197-012-0786-9.
[14]蔡铭,罗印龙,孙培龙. 黑木耳多糖的抑菌活性与单糖组分分析[J]. 浙江工业大学学报, 2014, 42(5): 533-538.
[15]袁海娜. 仙人掌及其多糖对几种食品污染菌的抑菌作用[J].食品研究与开发, 2012, 33(8): 10-13.
[16]胡国元,李超影,陈默,等.香菇多糖和金针菇多糖的提取及其抑菌活性[J].武汉工程大学学报, 2013, 35(6): 30-34.
[17]孟俊龙,冯翠萍,常明昌,等. 香菇多糖抑菌作用的研究[J].山西农业大学学报(自然科学版),2012, 32(3): 261-264.
[18]万艳娟,刘晓娟,赵力超,等. 南瓜多糖抑制α-淀粉酶及抑菌活性的研究[J]. 食品科技, 2012, 37(3): 44-47.
[19]陈灿辉,江文韬,林彤,等. 竹笋多糖的提取、结构鉴定与生理功效研究进展[J].江苏农业学报,2019,35(6):1513-1520.
[20]CHEN Q, YANG G W, AN L G. Apoptosis of hepatoma cells SMMC-7721 induced by Ginkgo biloba seed polysaccharide[J]. World Journal Gastroenterol, 2002, 8(5): 832-836.
[21]郑敏燕,耿薇,魏永生,等. 萘酚-硫酸显色光度法测定白果多糖含量方法的研究[J].应用化工,2010,39(3): 447-449,452.
[22]吴海霞,吴彩娥,范龚健,等. 银杏种仁蛋白分离纯化及其抑菌活性[J].食品科学, 2014, 35(13): 108-113.
[23]PIERRE G, SOPENA V, JUIN C, et al. Antibacterial activity of a sulfated galactan extracted from the marine alga Chaetomorpha aerea against Staphylococcus aureus[J]. Biotechnology and Bioprocess Engineering, 2011, 16(5): 937-945.

相似文献/References:

[1]陈灿辉,江文韬,林彤,等.竹笋多糖的提取、结构鉴定与生理功效研究进展[J].江苏农业学报,2019,(06):1513.[doi:doi:10.3969/j.issn.1000-4440.2019.06.034]
　CHEN Can-hui,JIANG Wen-tao,LIN Tong,et al.Progress in extraction, structural characterization and biological activities of bamboo shoot polysaccharide[J].,2019,(06):1513.[doi:doi:10.3969/j.issn.1000-4440.2019.06.034]
[2]林志銮,金晓怀,张传海,等.离子液体超声波辅助提取白花葛茎多糖工艺优化[J].江苏农业学报,2020,(01):187.[doi:doi:10.3969/j.issn.1000-4440.2020.01.026]
　LIN Zhi-luan,JIN Xiao-huai,ZHANG Chuan-hai,et al.Optimization of ionic liquid based ultrasonic-assisted extraction of polysaccharide from Pueraria f. niveus stem[J].,2020,(06):187.[doi:doi:10.3969/j.issn.1000-4440.2020.01.026]
[3]陈凌,曹巧巧,张建群.Box-Behnken中心组合设计优化酶法提取马齿苋多糖的工艺[J].江苏农业学报,2020,(02):500.[doi:doi:10.3969/j.issn.1000-4440.2020.02.034]
　CHEN Ling,CAO Qiao-qiao,ZHANG Jian-qun.Optimization on enzymatic extraction of polysaccharides from Portulaca oleracea L. by Box-Behnken central composite design[J].,2020,(06):500.[doi:doi:10.3969/j.issn.1000-4440.2020.02.034]
[4]石子林,李军乔,王雅琼,等.蕨麻地上部分多糖提取工艺优化及其抗氧化活性[J].江苏农业学报,2020,(03):720.[doi:doi:10.3969/j.issn.1000-4440.2020.03.026]
　SHI Zi-lin,LI Jun-qiao,WANG Ya-qiong,et al.Optimization on the extraction process of polysaccharide from the aerial part of Potentilla anserina L. and its antioxidant activity[J].,2020,(06):720.[doi:doi:10.3969/j.issn.1000-4440.2020.03.026]
[5]马明磊,朱强,王波.牛蒡根皮多酚、多糖粗提物对海兰褐壳蛋鸡产蛋性能及血液生化指标的影响[J].江苏农业学报,2022,38(04):1013.[doi:doi:10.3969/j.issn.1000-4440.2022.04.018]
　MA Ming-lei,ZHU Qiang,WANG Bo.Effects of polyphenols and polysaccharides from burdock root peel on egg production performance and blood biochemical indices of Hy-line brown laying hens[J].,2022,38(06):1013.[doi:doi:10.3969/j.issn.1000-4440.2022.04.018]

备注/Memo

备注/Memo:
收稿日期：2020-05-18基金项目：山西省高等学校科技创新项目(2019L0865)；山西省“1331”工程重点学科项目(098-091704)；运城学院博士科研启动项目(YQ-2017010)；运城学院学科群学科研究项目(XK-2018001、XK-2019034)；特色农产品加工山西省重点实验室开放课题项目；山西省重点研发计划重点项目(201903D211006)作者简介：吴海霞(1980-)，女，山西河津人，博士，副教授，主要研究方向为植物活性成分及其构效关系。(E-mail)whxviolet@163.com
更新日期/Last Update: 2021-01-15