[1]马明磊,朱强,王波.牛蒡根皮多酚、多糖粗提物对海兰褐壳蛋鸡产蛋性能及血液生化指标的影响[J].江苏农业学报,2022,38(04):1013-1020.[doi:doi:10.3969/j.issn.1000-4440.2022.04.018]
　MA Ming-lei,ZHU Qiang,WANG Bo.Effects of polyphenols and polysaccharides from burdock root peel on egg production performance and blood biochemical indices of Hy-line brown laying hens[J].,2022,38(04):1013-1020.[doi:doi:10.3969/j.issn.1000-4440.2022.04.018]
点击复制

牛蒡根皮多酚、多糖粗提物对海兰褐壳蛋鸡产蛋性能及血液生化指标的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年04期
页码:
1013-1020
栏目:
畜牧兽医·水产养殖
出版日期:
2022-08-31

文章信息/Info

Title:
Effects of polyphenols and polysaccharides from burdock root peel on egg production performance and blood biochemical indices of Hy-line brown laying hens
作者:
马明磊1; 朱强1; 王波2
（1.江苏海洋大学食品科学与工程学院，江苏连云港222005；2.徐州市检验检测中心，江苏徐州221000）
Author(s):
MA Ming-lei1; ZHU Qiang1; WANG Bo2
（1.School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang 222005, China；2.Xuzhou City Inspection and Testing Center, Xuzhou 221000, China）
关键词:
牛蒡根皮; 多酚; 多糖; 产蛋品质; 血清生化指标
Keywords:
burdock root peel; polyphenol; polysaccharide; egg quality; serum biochemical indicators
分类号:
S831.91
DOI:
doi:10.3969/j.issn.1000-4440.2022.04.018
文献标志码:
A
摘要:
以乙醇为溶剂提取牛蒡根皮中的多酚，用甲醇-乙酸乙酯对牛蒡根皮进行脱脂处理后，以水为溶剂提取牛蒡根皮中的多糖。分别将牛蒡根皮的多酚粗提物、多糖粗提物按照400 mg/kg的添加量添加于海兰褐壳蛋鸡基础日粮中，按照牛蒡根皮多酚粗提物组、牛蒡根皮多糖粗提物组、对照组（饲喂基础日粮）喂养40周龄的海兰褐壳蛋鸡，喂养期为30 d，分析日粮中添加牛蒡根皮多酚粗提物、多糖粗提物对海兰褐壳蛋鸡产蛋性能、蛋品质、血液生化指标的影响。结果表明，日粮中分别添加牛蒡根皮多酚粗提物、多糖粗提物均能提高海兰褐壳蛋鸡的产蛋性能，对总产蛋质量的影响显著（P<0.05）；与对照相比，日粮中分别添加牛蒡根皮多酚粗提物、多糖粗提物的软蛋率、破蛋率、畸形蛋率极显著（P<0.01）降低，但蛋壳质量分数未表现出显著差异，多酚粗提物组的哈夫单位显著提高（P<0.05）；日粮中添加牛蒡根皮多糖粗提物的海兰褐壳蛋鸡增长的体质量较对照显著（P<0.05）提高6.39%，日粮中添加牛蒡根皮多酚粗提物的海兰褐壳蛋鸡增长的体质量较对照显著（P<0.05）提高11.43%；与对照相比，日粮中添加牛蒡根皮多酚粗提物显著（P<0.05）降低了海兰褐壳蛋鸡血清中的谷丙转氨酶（ALT）活性、总胆固醇（Total cholesterol, TC） 浓度、低密度脂蛋白胆固醇（Low density lipoprotein cholesterol, LDL-C）浓度、磷（Phosphorus, Pi）浓度，极显著（P<0.01）提高了血清中的钙（Ca2+）浓度，日粮中添加牛蒡根皮多糖粗提物对以上指标的影响不显著。
Abstract:
Ethanol was used as solvent to extract polyphenols from burdock root peel. After degreasing with methanol-ethyl acetate, water was used as solvent to extract polysaccharides. Crude polyphenol extracts and crude polysaccharide extracts were added to the basic diet of Hy-line brown laying hens at the dosage of 400 mg/kg. The 40-week-old Hy-line brown laying hens were fed in crude polyphenol extracts group, crude polysaccharide extracts group and control group, and the feeding period was 30 days. Effects of crude polyphenol extracts and crude polysaccharide extracts on egg laying performance, egg quality and blood biochemical index of Hy-line brown laying hens were analyzed. The results showed that the addition of polyphenols and polysaccharide in the diet could improve the egg laying performance of laying hens, and had significant effect on the total egg weight （P<0.05）. Compared with the control, the soft egg rate, broken egg rate and deformed egg rate were significantly reduced in polyphenol and polysaccharide groups （P<0.01）, but there was no significant difference in eggshell mass fraction, and the Haugh unit in polyphenol group was significantly increased （P<0.05）. Compared with the control group, the increased body weight of Hy-line brown laying hens increased by 6.39% in polysaccharide group and 11.43% in polyphenol group. Compared with the control group, the addition of crude polyphenol extracts in the diet significantly decreased the activity of alanine aminotransferase(ALT), total cholesterol (TC) concentration, low-density lipoprotein cholesterol (LDL-C) concentration, phosphorus (Pi) concentration （P<0.05）, and extremely significantly increased the calcium (Ca2 +) concentration in serum （P<0.01）. The effect of polysaccharides on the above indicators was not significant.

参考文献/References:

[1]娄在祥. 牛蒡功能性成分及其抗氧化、抗菌活性研究[D]. 无锡:江南大学,2010.
[2]朱连连,栾晓宁,窦德强. 牛蒡根固体茶制剂中功能性成分含量测定[J].辽宁中医药大学学报,2019,21(2):66-69.
[3]MORO T M A, CELEGATTI C M, PEREIRA A P A, et al. Use of burdock root flour as a prebiotic ingredient in cookies[J]. LWT, 2018, 90: 540-546.
[4]冯营,胡新燕,孙亚伟,等. 种植密度对徐淮地区牛蒡产量与品质的影响[J]. 安徽农业科学,2018,46(10):66-67.
[5]冯营,胡新燕,孙亚伟,等. 江苏省徐州地区重要出口蔬菜牛蒡研究现状[J]. 园艺与种苗,2015(8):25-27.
[6]吴传华,冯沛,赵林,等. 江苏徐州出口牛蒡与大蒜生产加工关键技术研究[J]. 园艺与种苗，2016(10):66-68.
[7]马利华,宋慧,米莎莎. 几种处理方法对牛蒡多酚提取及抗氧化性的影响[J].中国食品添加剂,2017(8):191-196.
[8]刘群明,王燕,邱艳明,等. 响应面法优化牛蒡根总黄酮超生波提取工艺[J]. 农产品加工,2019(5):27-31.
[9]马天宇,陈燕平,程素盼,等. 牛蒡子研究进展[J]. 辽宁中医药大学学报,2018,20(9):113-116.
[10]YU Y, SHEN M Y, SONG Q, et al. Biologocal activities and pharmaceutical applications of polysaccharide from natural resources[J]. Carbohydrate Polymers, 2018(183):91-101.
[11]SHI L. Bioactivities, isolation and purification methods of polysaccharides from natural products[J]. International Journal of Biological Macromolecules, 2016(92):37-48.
[12]JIANG Y Y, YU J, LI Y B, et al. Extraction and antioxidant activities of polysaccharides from roots of Arctium lappa L.[J]. International Journal of Biological Macromolecules, 2019(123):531-538.
[13]JIANG X W, BAI J P, ZHANG Q, et al. Caffeoylquinic acid derivatives from the roots of Arctium lappa L. (burdock) and their structure-activity relationships (SARs) of free radical scavenging activities[J]. Phytochemistry Letters, 2016(15):159-163.
[14]MCCREA G L, MIASKOWSKI C, STOTTS N A, et al. Pathophysiology of constipation in the older adult[J]. World Journal of Gastroenterology, 2008, 14(17):2631-2638.
[15]PEREIRA J V, BERGAMO D C, PEREIRA J O, et al. Antimicrobiol activity of Arctium lappa constituents against microorganisms commonly found in endodontic infections[J]. Brazilian Dental Journal, 2005,16(3):192-196.
[16]WU Y, GUO R, CAO N, et al. A systematical rheological study of polysaccharide from Sophora alopecuroides L. seeds[J]. Carbohydrate Polymers, 2018，180：63-71.
[17]RAJASEKHARAN S K, RAMESH S, SATISH A S, et al. Antibiofilm and anti-β-lactamase activities of burdock root extractand chlorogenic acid against Klebsiella pneumoniae[J]. Microbiol Biotechnol, 2017,27(3):542-551.
[18]RAJASEKHARAN S K, RAMESH S, BAKKIYARAJ D, et al. Burdock root extracts limitquorum-sensing-controlled phenotypes and biofilm architecturein major urinary tract pathogens[J]. Urolithiasis, 2015, 43(1): 29-40.
[19]RODRIGUEZ J M F, SOUZA A R C, KRGER R L, et al. Kinetics, composition and antioxidant activity of burdock (Arctium lappa) root extracts obtained with supercritical CO2 and co-solvent [J]. The Journal of Supercritical Fluids, 2018, 135: 25-33.
[20]马利华,秦卫东,陈学红,等. 牛蒡皮提取物抗氧化活性及组成的研究[J].中国食品学报, 2011,11(8): 23-29.
[21]马艳弘,孟勇,崔晋,等. 牛蒡多酚超声辅助酶法提取工艺及抗氧化活性[J].食品与生物技术学报,2020,39(1):38-45.
[22]俞燕. 肉仔鸡饲喂蘑菇提取物可增加粪便中双歧杆菌数[J].中国家禽,2010,32(1):67.
[23]黄贺儒. 中草药添加剂在蛋鸡上的应用效果[J].兽药与饲料添加剂,2002(5):2-3.
[24]马玉胜,张玲,宋照霞. 牛蒡根下脚料饲喂产蛋鸡的效果试验[J].中国禽业导刊,2000（18）:24.
[25]葛蕾. 苹果渣多酚浸提物及其抗氧化性研究[D].杨凌:西北农林科技大学,2005．
[26]冯以明,李广生,吴建东,等. 雨生红球藻多糖的提取分离及理化性质研究[J].海洋科学,2012, 36(1):17-22.
[27]王家辉,刘杉杉,张鑫,等. 牛蒡不同部位多糖的抗氧化与抗凝血活性研究[J].食品工业科技,2020,41(6):305-310.
[28]LI X P, WANG J, ZHANG H, et al. Renoprotective effect of low-molecular-weight sulfated polysaccharide from the seaweed Laminaria japonica on glycerol-induced acute kidney injury in rats[J].International Journal of Biological Macromolecules, 2017, 95:132-137.
[29]李瑾,吴理娟. 牛蒡根总多糖的提取研究[J].首都食品与医药,2009,16(14):53.
[30]张利娟. 中草药提取物复方对蛋鸡产蛋后期鸡饲喂效果研究[D].郑州:河南农业大学,2010.
[31]岳道友,李亚明,赵杰. 益生菌与中草药对海兰褐蛋鸡生产性能及蛋品质的影响[J]. 河南农业大学学报, 2017, 51(3):370-373.
[32]李晓,廖智慧,陈志军,等. 饲料中添加紫花苜蓿多糖对肉羊生长性能和血清中生化指标影响[J].中国饲料,2018(23):40-43.
[33]欧淑琦,刘祝英,郭丹,等. 血根碱和牛膝多糖对肉鸡血液生化指标及免疫性能的影响[J].中国兽医学报,2019,39(10):2055-2060.
[34]FAN W T, ZHENG P M, WANG Y, et al, Analysis of immunostimulatory activity of polysaccharide extracted from Yu-Ping-Feng in vitro and in vivo[J]. Biomedicine ＆ Pharmacotherapy, 2017,93:146-155.
[35]FAN Y P, MA X, MA L, et al. Antioxidative and immunological activity of Ophiopogon polysaccharide liposome from the root of Ophiopogon japonicus[J].Carbohydrate Polymers,2016,135:110-120.

相似文献/References:

[1]李洁,王沂雯,郑涵予,等.余甘子多酚微波-热水浸提工艺的优化及其pH稳定性[J].江苏农业学报,2020,(03):733.[doi:doi:10.3969/j.issn.1000-4440.2020.03.028]
　LI Jie,WANG Yi-wen,ZHENG Han-yu,et al.Optimization of microwave-hot water extraction process of polyphenols from Phyllanthus emblica L. and its pH stability[J].,2020,(04):733.[doi:doi:10.3969/j.issn.1000-4440.2020.03.028]
[2]刘佩,杜国荣,赵鹏涛,等.9种植物源多酚的分离提取及其涩感评价[J].江苏农业学报,2021,(04):1025.[doi:doi:10.3969/j.issn.1000-4440.2021.04.027]
　LIU Pei,DU Guo-rong,ZHAO Peng-tao,et al.Separation and extraction of nine plant polyphenols and evaluation of astringency[J].,2021,(04):1025.[doi:doi:10.3969/j.issn.1000-4440.2021.04.027]
[3]张家旭,王信,叶倩女,等.松针多酚提取工艺优化及其抗氧化、酶活性抑制特征[J].江苏农业学报,2023,(07):1593.[doi:doi:10.3969/j.issn.1000-4440.2023.07.016]
　ZHANG Jia-xu,WANG Xin,YE Qian-nü,et al.Optimization of extraction process of polyphenols from pine needles and its antioxidant and enzyme activity inhibitory characteristics[J].,2023,(04):1593.[doi:doi:10.3969/j.issn.1000-4440.2023.07.016]

备注/Memo

备注/Memo:
收稿日期：2021-12-24基金项目：江苏省研究生科研与实践创新计划项目（SJCX20_1314）作者简介：马明磊（1982-），男，江苏徐州人,硕士研究生，助理工程师，研究方向为食品生物技术。(E-mail)903638085@qq.com通讯作者：朱强，(E-mail)zq7685@163.com
更新日期/Last Update: 2022-09-06