[1]张敬峰,董永毅,卢凤英,等.鸭疫里氏杆菌1型流行株的生物学特性及免疫原性[J].江苏农业学报,2022,38(04):1021-1025.[doi:doi:10.3969/j.issn.1000-4440.2022.04.019]
　ZHANG Jing-feng,DONG Yong-yi,LU Feng-ying,et al.Biological characteristics and immunogenicity of Riemerella anatipestifer serotype 1 epidemic strains[J].,2022,38(04):1021-1025.[doi:doi:10.3969/j.issn.1000-4440.2022.04.019]
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鸭疫里氏杆菌1型流行株的生物学特性及免疫原性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年04期
页码:
1021-1025
栏目:
畜牧兽医·水产养殖
出版日期:
2022-08-31

文章信息/Info

Title:
Biological characteristics and immunogenicity of Riemerella anatipestifer serotype 1 epidemic strains
作者:
张敬峰1; 董永毅2; 卢凤英1; 刘青涛1; 徐彬1; 赵莎1; 孙华伟1;  吴坤2; 张小飞1
（1.江苏省农业科学院兽医诊断检测中心，江苏南京210014；2.江苏省动物疫病预防控制中心，江苏南京210036）
Author(s):
ZHANG Jing-feng1; DONG Yong-yi2; LU Feng-ying1; LIU Qing-tao1; XU Bin1; ZHAO Sha1; SUN Hua-wei1; WU Kun2; ZHANG Xiao-fei1
(1.Veterinary Diagnostic Testing Center， Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Jiangsu Center for Animal Disease Control and Prevention, Nanjing 210036, China)
关键词:
鸭疫里氏杆菌1型; 生物学特性; 交叉免疫保护
Keywords:
Riemerella anatipestifer serotype 1; biological characteristics; cross-immune protection
分类号:
S858.322.61
DOI:
doi:10.3969/j.issn.1000-4440.2022.04.019
文献标志码:
A
摘要:
为研究当前鸭疫里氏杆菌1型（RA1）流行株的生物学特性及免疫原性，对从江苏省、山东省等不同地区规模鸭场采集的病料中分离的23个疑似RA的菌株纯化培养后进行细菌形态观察，并利用RA 16S rRNA基因特异性引物进行PCR鉴定及基因序列测定、生化试验、血清学分型鉴定、致病性分析、交叉攻毒保护试验。结果表明，16株分离株符合RA生物学特性，经PCR检测为RA阳性，与GenBank中RA相应基因同源性≥99%，其中11株血清学鉴定为RA1，占分离RA总数的68.7%；选取的5株RA1分离株中JP01、SD02、GY01等3株致病率和致死率达80％～100％；对3株RA1分离株进行免疫原性研究，结果显示JP01株对各毒株的交叉攻毒保护率可达100％，说明JP01株具有良好的免疫原性。
Abstract:
In order to study the biological characteristics and immunogenicity of Riemerella anatipestifer serotype 1 (RA1) epidemic strains, 23 strains of suspected RA isolated from disease materials collected from duck farms in different regions of Jiangsu, Shandong and other provinces were purified and cultured. Subsequently, these bacteria were subjected to morphological observation, PCR identification， gene sequencing, biochemical tests, serological identification, pathogenicity tests and cross-challenge protection tests by using RA 16S rRNA gene-specific primers. The results showed that 16 isolates were in line with the biological characteristics of RA and were identified as positive by PCR. The homology with the corresponding genes of RA in GenBank was not less than 99%. Among them, 11 strains were serologically identified as RA1, accounting for 68.7% of the total isolated RA. Among the five selected RA1 isolates, the morbidity and mortality of JP01, SD02 and GY01 ranged from 80% to 100%. The immunogenicity study of three RA1 isolates showed that the cross-challenge protection rate of JP01 strain against each virulent strain could reach 100%, indicating that JP01 strain had good immunogenicity.

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备注/Memo

备注/Memo:
收稿日期：2021-11-30基金项目：江苏现代农业（水禽）产业技术体系疾病防控创新团队项目[JATS(2021)357]；江苏省农业科技自主创新基金项目 [CX（19）3002]作者简介：张敬峰 (1982-),男,安徽含山人,硕士,副研究员,主要从事畜禽重大疫病防控技术及兽用生物制品研究。(Tel)025-84390131;(E-mail)42642098@qq.com
更新日期/Last Update: 2022-09-06