[1]纪伟,苏文英,刘晓梅,等.连云港地区野生灵芝生物学特性及基因组特征分析[J].江苏农业学报,2024,(02):223-232.[doi:doi:10.3969/j.issn.1000-4440.2024.02.004]
 JI Wei,SU Wen-ying,LIU Xiao-mei,et al.Analysis of biological characteristics and genomic characteristics of wild Ganoderma lucidum in Lianyungang[J].,2024,(02):223-232.[doi:doi:10.3969/j.issn.1000-4440.2024.02.004]
点击复制

连云港地区野生灵芝生物学特性及基因组特征分析()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年02期
页码:
223-232
栏目:
遗传育种·生理生化
出版日期:
2024-02-25

文章信息/Info

Title:
Analysis of biological characteristics and genomic characteristics of wild Ganoderma lucidum in Lianyungang
作者:
纪伟12苏文英1刘晓梅1任立凯1胡曙鋆3孙潇潇3陈克龙2
(1.连云港市农业科学院,江苏连云港222000;2.青海师范大学,青海西宁810000;3.连云港市园艺蔬菜指导站,江苏连云港222000)
Author(s):
JI Wei12SU Wen-ying1LIU Xiao-mei1REN Li-kai1HU Shu-yun3SUN Xiao-xiao3CHEN Ke-long2
(1.Lianyungang Academy of Agricultural Sciences, Lianyungang 222000, China;2.Qinghai Normal University, Xining 810000, China;3.Lianyungang Gardening Vegetable Guidance Station, Lianyungang 222000, China)
关键词:
灵芝生物学特性全基因组重测序主成分分析
Keywords:
Ganoderma lucidumbiological characteristicswhole genome resequencingprincipal component analysis
分类号:
S567.3+1
DOI:
doi:10.3969/j.issn.1000-4440.2024.02.004
摘要:
野生灵芝资源在连云港境内有着广泛的分布,本研究使用全基因组重测序技术对具有不同形态特征和生境特点的灵芝基因组进行测序及分析,以期解析连云港地区野生灵芝的生物学特性及基因组特征。结果表明,从不同区域采集的野生灵芝种质资源菌丝具有锁状联合,具备形成子实体的条件,生物学特性存在较大差异;通过主成分分析将12株野生灵芝菌株种质资源聚为3类,其中第Ⅲ类具有更丰富的遗传多样性;比较单核苷酸多态性(SNP)、插入或者缺失(InDel)在12株灵芝菌株基因组中的分布情况发现,第1条染色体中的SNP最多,占比为46.07%,SNP中转换颠换比(Ts/Tv)为2.03,说明变异以转换为主,插入或缺失导致的基因差异在第1条染色体上最多,占总InDel数的44.31%。研究结果说明,连云港地区灵芝具有丰富的遗传多样性,可以用于进一步挖掘更多的功能基因和进行品种选育。
Abstract:
Wild Ganoderma lucidum resources are extensively found in Lianyungang. In this study, we utilized whole-genome resequencing technology to examine and analyze the genomes of Ganoderma lucidum with different morphological and habitat characteristics. The purpose of this study was to unveil the biological and genomic characteristics of wild Ganoderma lucidum in Lianyungang. The results showed that the mycelium of wild Ganoderma lucidum germplasm resources collected from different regions had clamp connection, and had the conditions to form fruiting bodies, and the biological characteristics were quite different. Through principal component analysis, 12 wild Ganoderma lucidum germplasm resources were grouped into three categories, and the third category had more abundant genetic diversity. Analyzing the distribution of single nucleotide polymorphism (SNP) and insertion or deletion (InDel) in the genome of 12 strains of Ganoderma lucidum, it was found that the number of SNP on chromosome 1 was the largest, accounting for 46.07%, and the conversion and inversion ratio (Ts/Tv) in the SNP was 2.03, indicating that the variation was mainly conversion. The genetic difference caused by insertion or deletion was the highest on chromosome 1, accounting for 44.31% of the total InDel number. These results showed that Ganoderma lucidum in Lianyungang had rich genetic diversity, which could be used to further explore more functional genes and breed varieties.

参考文献/References:

[1]胡惠萍,刘远超,莫伟鹏,等. 两株西藏白肉灵芝菌株特性初探[J]. 食用菌学报,2017,24(1):50-54.
[2]戴玉成,曹云,周丽伟,等. 中国灵芝学名之管见[J]. 菌物学报,2013,32(6):947-952.
[3]SOHRETOGLU D, HUANG S. Ganoderma lingzhi polysaccharides as an anti-cancer agent[J]. Anti-Cancer Agents in Medicinal Chemistry,2018,18(5):667-674.
[4]王朝川. 灵芝成分及功能的研究现状[J]. 中国果菜,2018,38(8):45-47,53.
[5]MENG J, WANG S Z, HE J Z, et al. Ganoderic acid A is the effective ingredient of Ganoderma triterpenes in retarding renal cyst development in polycystic kidney disease[J]. International Journal of Sports Medicine,2020,41(6):782-790.
[6]李丹妮,朱长俊,郑雨晴,等. 灵芝种植及初加工研究进展[J]. 南方农业,2021,15(8):233-235.
[7]崔黎明,刘利,潘丽艳,等. 灵芝孢子误诊为寄生虫虫卵3例分析[J]. 中国实验诊断学,2016,20(7):1191-1192.
[8]蒙田秀,杨力龙,龚志强,等. 壮族药白鹤灵芝萘醌类化学成分及其药理作用研究进展[J]. 中国实验方剂学杂志,2020,26(10):213-219.
[9]王梦晨,张雪涟,陈向东,等. 灵芝三萜与灵芝多糖抗肿瘤作用及其机制研究进展[J]. 中国实验方剂学杂志,2022,28(5):234-241.
[10]CHEN L H, ABUDUMIJITI A, LI M. Protective effect of Ganoderma (Lingzhi) on radiation and chemotherapy[J]. Advances in Experimental Medicine and Biology,2019,1182:119-142.
[11]REN L, ZHANG J, ZHANG T H. Immunomodulatory activities of polysaccharides from Ganoderma on immune effector cells[J]. Food Chemistry,2021,340:127933.
[12]庄静. 试论中药灵芝补肺汤在支气管哮喘病例治疗中的临床应用效果观察[J]. 中国医药指南,2017,15(21):183-184.
[13]沈滟惠,温亚格,唐庆九,等. 灵芝抗肿瘤活性及其机制研究进展[J]. 海峡药学,2020,32(4):8-12.
[14]兰慧莹,毛可红,陈俏彪,等. 工艺灵芝的制作技术刍议[J]. 食药用菌,2019,27(6):427-429.
[15]唐才明,张燕,陈娇娇,等. CO2缓释剂在盆栽灵芝中应用初探[J]. 食用菌,2019,41(2):55-56, 65.
[16]豆芬芬. 连云港港口与城市耦合协调发展研究[D]. 徐州:中国矿业大学,2014.
[17]林祥天,邵世光,吕继江,等. 连云港地区食用菌和毒菌的研究[J]. 江苏预防医学,1997(4):13-14.
[18]邢炜,洪豆,徐碧林,等. 黄冈大别山一株野生灵芝的鉴定与驯化栽培[J]. 中国食用菌,2021,40(10):28-33.
[19]LIU Y C, HUANG L H, HU H P, et al. Whole-genome assembly of Ganoderma leucocontextum (Ganodermataceae, Fungi) discovered from the Tibetan Plateau of China[J]. G3 Genes|Genomes|Genetics,2021,11(12):jkab337.
[20]鲍大鹏. 食用菌杂交育种中的科学问题[J]. 食用菌学报,2020,27(4):1-24.
[21]张先吉,花纪,颜俊清,等. 几株主栽灵芝菌株液体菌种培养基优化及其活力评价[J]. 食品科技,2020,45(4):19-25.
[22]韩鹏,陈青君,贺国强,等. 2种野生羊肚菌分离、鉴定与菌丝培养条件[J]. 北京农学院学报,2018,33(1):37-42.
[23]董玉兰,李书生,张丽萍,等. 白灵菇高活力液体菌种摇瓶发酵培养条件的优化[J]. 北方园艺,2014(6):143-146.
[24]曲德辉,杨焱,张赫男,等. 桑黄菌株活力评价及优良菌株筛选[J]. 菌物学报,2016,35(10):1187-1198.
[25]BROWN N, BRANCH W D, JOHNSON M, et al. Genetic diversity assessment of Georgia peanut cultivars developed during ninety years of breeding[J]. The Plant Genome,2021,14(3):e20141.
[26]郭敏杰,邓丽,李玉荣,等. 基于主成分和聚类分析的冀花高油酸花生品种综合评价[J]. 中国油料作物学报,2022,44(6):1210-1217.
[27]OGDEN R, GHARBI K, MUGUE N, et al. Sturgeon conservation genomics:SNP discovery and validation using RAD sequencing[J]. Molecular Ecology,2013,22(11):3112-3123.
[28]SHEN C, JIN X, ZHU D, et al. Uncovering SNP and indel variations of tetraploid cottons by SLAF-seq[J]. BMC Genomics,2017,18:247.
[29]ZHANG X F, WANG G Y, CHEN B, et al. Candidate genes for first flower node identified in pepper using combined SLAF-seq and BSA[J]. PLoS One,2018,13(3):e0194071.

相似文献/References:

[1]张俊喜,成晓松,宋益民,等.中国水稻稻曲病研究进展[J].江苏农业学报,2016,(01):234.[doi:10.3969/j.issn.1000-4440.2016.01.037 ]
 ZHANG Jun-xi,CHENG Xiao-song,SONG Yi-min,et al.Research advances in rice false smut (Ustilaginoidea virens) in China[J].,2016,(02):234.[doi:10.3969/j.issn.1000-4440.2016.01.037 ]
[2]刘昭,于小番,张宇,等.丝氨酸蛋白酶编码基因prtP对副干酪乳杆菌黏附特性的影响[J].江苏农业学报,2017,(03):683.[doi:doi:10.3969/j.issn.1000-4440.2017.03.029]
 LIU Zhao,YU Xiao-fan,ZHANG Yu,et al.Adhesion characteristics of Lactobacillus paracasei influenced by serine protease encoding gene prtP[J].,2017,(02):683.[doi:doi:10.3969/j.issn.1000-4440.2017.03.029]
[3]钟希娜,何涛,葛展霞,等.一种对mcr-1阳性大肠杆菌具有裂解作用的噬菌体生物学特性[J].江苏农业学报,2018,(02):452.[doi:doi:10.3969/j.issn.1000-4440.2018.02.033]
 ZHONG Xi-na,HE Tao,GE Zhan-xia,et al.Characterization of a lytic bacteriophage infecting mcr-1-positive Escherichia coli[J].,2018,(02):452.[doi:doi:10.3969/j.issn.1000-4440.2018.02.033]
[4]葛展霞,钟希娜,朱炳海,等.NDM-5阳性大肠杆菌裂解性噬菌体的生物学特性[J].江苏农业学报,2018,(03):699.[doi:doi:10.3969/j.issn.1000-4440.2018.03.032]
 GE Zhan-xia,ZHONG Xi-na,ZHU Bing-hai,et al.The biological characteristics of a bacteriophage against NDM-5 positive Escherichia coli[J].,2018,(02):699.[doi:doi:10.3969/j.issn.1000-4440.2018.03.032]
[5]李陇平,杨吉,白蹉蹉,等.一株绒山羊源大肠杆菌噬菌体φPTK的分离鉴定及生物学特性分析[J].江苏农业学报,2018,(04):847.[doi:doi:10.3969/j.issn.1000-4440.2018.04.019]
 LI Long-ping,YANG Ji,BAI Cuo-cuo,et al.Isolation, identification and biological properties of a lytic phage against goat (Capra hircus)-associated Escherichia coli[J].,2018,(02):847.[doi:doi:10.3969/j.issn.1000-4440.2018.04.019]
[6]罗怡琳,蒙正群,周丽军,等.兔脾脏成纤维型细胞RS-17系的建立及其生物学特性[J].江苏农业学报,2019,(01):130.[doi:doi:10.3969/j.issn.1000-4440.2019.01.019]
 LUO Yi-lin,MENG Zheng-qun,ZHOU Li-jun,et al.Establishment and biological characteristics of rabbit spleen fibroblast cell line RS-17[J].,2019,(02):130.[doi:doi:10.3969/j.issn.1000-4440.2019.01.019]
[7]肖琦,赵霞玲,钱雯娴,等.猪链球菌3型分离菌的生物学特性及致病性[J].江苏农业学报,2020,(06):1475.[doi:doi:10.3969/j.issn.1000-4440.2020.06.017]
 XIAO Qi,ZHAO Xia-ling,QIAN Wen-xian,et al.Biological characteristics and pathogenicity of Streptococcus suis serotype 3 isolates[J].,2020,(02):1475.[doi:doi:10.3969/j.issn.1000-4440.2020.06.017]
[8]张敬峰,董永毅,卢凤英,等.鸭疫里氏杆菌1型流行株的生物学特性及免疫原性[J].江苏农业学报,2022,38(04):1021.[doi:doi:10.3969/j.issn.1000-4440.2022.04.019]
 ZHANG Jing-feng,DONG Yong-yi,LU Feng-ying,et al.Biological characteristics and immunogenicity of Riemerella anatipestifer serotype 1 epidemic strains[J].,2022,38(02):1021.[doi:doi:10.3969/j.issn.1000-4440.2022.04.019]
[9]王森,田俊,刘曼.甘薯长喙壳菌的致病机制、毒素合成及防控研究进展[J].江苏农业学报,2023,(05):1256.[doi:doi:10.3969/j.issn.1000-4440.2023.05.019]
 WANG Sen,TIAN Jun,LIU Man.Research progress on pathogenicity, toxin production, and control measures of Ceratocystis fimbriata[J].,2023,(02):1256.[doi:doi:10.3969/j.issn.1000-4440.2023.05.019]

备注/Memo

备注/Memo:
收稿日期:2023-02-13基金项目:江苏现代农业(蔬菜)产业技术体系[JATS(2022)177];连云港市财政专项(QNJJ2209);连云港市食用菌全产业链建设项目[连农复(2023)14号]作者简介:纪伟(1989-),男,江苏睢宁人,博士研究生,高级工程师,主要从事菌物资源研究与利用工作。(E-mail)jiwei100500@163.com通讯作者:任立凯,(E-mail)2949823@qq.com;陈克龙,(E-mail)ckl7813@163.com
更新日期/Last Update: 2024-03-17