[1]林志銮,金晓怀,张传海,等.离子液体超声波辅助提取白花葛茎多糖工艺优化[J].江苏农业学报,2020,(01):187-193.[doi:doi:10.3969/j.issn.1000-4440.2020.01.026]
 LIN Zhi-luan,JIN Xiao-huai,ZHANG Chuan-hai,et al.Optimization of ionic liquid based ultrasonic-assisted extraction of polysaccharide from Pueraria f. niveus stem[J].,2020,(01):187-193.[doi:doi:10.3969/j.issn.1000-4440.2020.01.026]
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离子液体超声波辅助提取白花葛茎多糖工艺优化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年01期
页码:
187-193
栏目:
加工贮藏·质量安全
出版日期:
2020-02-29

文章信息/Info

Title:
Optimization of ionic liquid based ultrasonic-assisted extraction of polysaccharide from Pueraria f. niveus stem
作者:
林志銮1金晓怀2张传海3马春华3
(1.武夷学院分析测试中心,福建南平354300;2.武夷学院玉山健康管理学院,福建南平354300;3.福建省生态产业绿色技术重点实验室(武夷学院),福建南平354300)
Author(s):
LIN Zhi-luan1JIN Xiao-huai2ZHANG Chuan-hai3MA Chun-hua3
(1.Analysis and Testing Center, Wuyi University, Nanping 354300, China;2.Yushan Health Management College, Wuyi University, Nanping 354300, China;3.Fujian Provincial Key Laboratory of Eco-Industrial Green Technology, Wuyi University, Nanping 354300, China)
关键词:
白花葛多糖离子液体超声波响应面
Keywords:
Pueraria f. niveuspolysaccharideionic liquidultrasonicresponse surface
分类号:
Q946.3
DOI:
doi:10.3969/j.issn.1000-4440.2020.01.026
文献标志码:
A
摘要:
利用超声波辅助提取多糖的方法,研究武夷山白花葛茎多糖的最优提取工艺条件。在单因素试验基础上,以白花葛茎多糖提取率为指标,进行四因素三水平Box-Behnken设计,用响应面法分析4个因素对白花葛茎多糖提取率的影响,并进行优化。最终以6%离子液体[BMIm][PF6] 3 ml、超声功率630 W、超声时间25 min、料液比1∶35(g/ml)为最佳提取条件,采用最优提取工艺条件对白花葛茎多糖进行提取,此时白花葛茎多糖的提取率为22.53 mg/g。扫描电镜观察发现,离子液体超声处理对白花葛茎粉末微观结构破坏程度较大。
Abstract:
The optimal extraction conditions of polysaccharide from Pueraria f. niveus stem in Wuyi Mountain were studied by ultrasonic assisted extraction method. On the basis of single-factor experiment, the extraction rate of polysaccharides from the Pueraria f.niveus was determined as the index. Box-behnken was designed with four factors at three levels. Response surface method was used to analyze the effect of four factors on the extraction rate of polysaccharides from the stem of Pueraria f.niveus, and the optimization was carried out. The optimal extraction conditions were as follows: the 6% ionic liquid [BMIm][PF6] was 3.00 ml, ultrasonic power was 630 W, ultrasonic extraction time was 25 min, the solid-liquid ratio was 1∶35. Under the optimal extraction conditions, the extraction rate of polysaccharide from the stem of Pueraria f. niveus was 22.53 mg/g. Besides, the scanning electron microscope observation results showed that the microstructure of the Pueraria f. niveus was destroyed greatly by ionic liquid ultrasonic treatment.

参考文献/References:

[1]李臻,赖富饶,吴晖.葛根的营养成分分析[J].现代食品科技,2011,12(8):1010-1012.
[2]阮淑明.白花葛的特征特性及栽培技术[J].现代农业科技, 2010(20):153-154.
[3]陈兵兵.葛根多糖的提取分离、理化特性及生物活性研究[D].镇江:江苏大学,2016.
[4]潘俊,匡慕予,田浩,等.粉葛中葛根素提取工艺的优化及其提取物抗氧化活性[J].中成药, 2018,40(11): 2430-2436.
[5]董洲.野葛根多糖的提取、分离纯化、结构鉴定及对小鼠巨噬细胞RAW264.7的免疫调节活性研究[D].广州:华南理工大学,2018.
[6]裴莉昕,纪宝玉,陈随清,等.葛根多糖提取工艺的优化[J].中国现代中药,2017,19(4): 553-556,567.
[7]周丽明,张勇,林国卫.等.葛根多糖提取条件的优化及其抗氧化活性的研究[J].湖北农业科学,2012,51(19):4344-4347.
[8]赵丹,聂波,宋昆,等.离子色谱法测定不同产地葛根多糖中的单糖组成[J].分析试验室,2017,36(7):745-749.
[9]张传海,林志銮,李宝银,等.闽北林下种植多花黄精的总黄酮含量分析及其生物活性评价[J].天然产物研究与开发,2018,30(2):225-231.
[10]李国明,张丽萍,戴余波,等.苯酚-硫酸比色法测定瑞丽白芨多糖含量[J].热带农业科学,2018,38(10):62-67.
[11]刘宝亮,吴一帆,穰小东,等.响应面法优化离子液体双水相提取银杏黄酮的工艺研究[J].中国食品添加剂,2018(12):108-115.
[12]粟敏,陈琳,龙昱,等.离子液体-微波辅助提取多花黄精多糖工艺研究[J].中药材,2016,39(9):2075-2077.
[13]封易成,牟德华.离子液体酶法微波辅助提取山楂有机酸工艺优化及成分分析[J].食品研究与开发,2019,40(10):87-95.
[14]USUKI T, YASUDA N, YOSHIZAWA-FUJITA M, et al. Extraction and isolation of shikimic acid from Ginkgo biloba leaves utilizing an ionic liquid that dissolves cellulose[J]. Chemical Communications, 2011, 47(38): 10560-10562.
[15]TAN T, LAI C J S, OUYANG H, et al. Ionic liquid-based ultrasound assisted extraction and aqueous two phase system for analysis of caffeoylquinic acids from Flos Lonicerae Japonicae [J]. Journal of Pharmaceutical Biomedical Analysis, 2016, 120: 134-141.
[16]徐慧,赵双枝,刘孝永,等.响应面法优化鹿角灵芝产胞内多糖的液体发酵工艺[J].江苏农业科学,2018,46(23) : 215-220.
[17]苏适,赵东江,王喜庆,等.响应面法优化超声辅助离子液体提取黑豆异黄酮及其抗氧化活性研究[J].中国粮油学报,2019,34(11):24-28.
[18]李明英.离子液体在天然活性物质提取中的应用研究进展[J].药学进展,2015,39(6):437-445.
[19]安仕豪,赵芳芳,魏佳,等.响应面法优化超声辅助离子液体提取籽瓜果胶工艺的研究[J].食品工业科技,2019,38(11):270-275.
[20]王刚,黎丹,蒋奉秦,等.离子液体结合微波提取石上柏中穗花杉双黄酮工艺优化[J].现代化工,2018,39(20):141-145.
[21]丁佳,曾珊,潘浪胜,等.响应曲面优化花生壳中木犀草素的提取工艺研究[J].现代化工,2017,37(8):121-126.
[22]白文强,王强,刘丽,等. 以离子液体为介质的修饰改性对多糖结构与理化性质的影响研究进展[J].食品科学,2018,39(17):291-297.

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备注/Memo

备注/Memo:
收稿日期:2019-07-10基金项目:福建省科技厅项目(WYKF2017-8、2018L3018、2017Y00076);武夷学院校级课题(XP201807)作者简介:林志銮(1981-),男,福建福州人,硕士,高级实验师,主要从事天然产物研究与开发和色谱分析研究。(E-mail)158843453@qq.com通讯作者:张传海,(E-mail)190070900@qq.com
更新日期/Last Update: 2020-03-13