[1]石子林,李军乔,王雅琼,等.蕨麻地上部分多糖提取工艺优化及其抗氧化活性[J].江苏农业学报,2020,(03):720-726.[doi:doi:10.3969/j.issn.1000-4440.2020.03.026]
 SHI Zi-lin,LI Jun-qiao,WANG Ya-qiong,et al.Optimization on the extraction process of polysaccharide from the aerial part of Potentilla anserina L. and its antioxidant activity[J].,2020,(03):720-726.[doi:doi:10.3969/j.issn.1000-4440.2020.03.026]
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蕨麻地上部分多糖提取工艺优化及其抗氧化活性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年03期
页码:
720-726
栏目:
加工贮藏·质量安全
出版日期:
2020-06-30

文章信息/Info

Title:
Optimization on the extraction process of polysaccharide from the aerial part of Potentilla anserina L. and its antioxidant activity
作者:
石子林123李军乔123王雅琼123董浩123牛永昆123李晨芹123
(1.青海民族大学青藏高原蕨麻研究中心,青海西宁810000;2.青海省生物技术与分析测试重点实验室,青海西宁810000;3.青海民族大学生态环境与资源学院,青海西宁810000)
Author(s):
SHI Zi-lin123LI Jun-qiao123WANG Ya-qiong123DONG Hao123NIU Yong-kun123LI Chen-qin123
(1.Tibetan Plateau Juema Research Centre, Qinghai University for Nationalities, Xining 810000, China;2.Qinghai Provincial Biotechnology and Analytical Test Key Laboratory, Xining 810000, China;3.College of Ecological Environment and Resources, Qinghai University for Nationalities, Xining 810000, China)
关键词:
蕨麻多糖响应面法提取工艺抗氧化活性
Keywords:
Potentilla anserina L.polysaccharideresponse surface methodextraction processantioxidant activity
分类号:
S566.9
DOI:
doi:10.3969/j.issn.1000-4440.2020.03.026
文献标志码:
A
摘要:
通过优化蕨麻地上部分多糖提取工艺,为蕨麻地上部分的综合利用提供试验依据。本研究以蕨麻地上部分多糖提取率为评估指标,以液料比、提取温度、提取时间为影响因素,在单因素试验基础上结合响应面法优化蕨麻地上部分多糖提取工艺,并采用2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除法以及1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除法测定其抗氧化活性。最佳提取工艺条件为:液料比41∶1(ml/g),提取温度80 ℃,提取时间97 min,提取率为2.68%。在最佳提取工艺条件下,多糖对DPPH自由基、ABTS自由基的半抑制质量浓度分别为0.76 mg/ml、0.64 mg/ml。本研究采用响应面法得到蕨麻地上部分多糖的最佳提取工艺,该工艺简便可行,提取的多糖具有较强的抗氧化活性。
Abstract:
To provide experimental basis for comprehensive utilization of the aerial parts of Potentilla anserina L., the extraction process of polysaccharide was optimized. In this study, the yield of polysaccharide was used as the evaluation index, and the liquid-solid ratio, extraction time and extraction temperature were taken as influencing factors. The extraction process was optimized by response surface methodology on the basis of single factor test. The antioxidant activity of polysaccharide was determined by 2, 2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging methods. The optimum extraction conditions were as follows: liquid-solid ratio 41∶1(ml/g), extraction temperature 80 ℃, extraction time 97 min. Under the above conditions, the yield of polysaccharide was 2.68%. Under the optimum extraction conditions, the half-inhibiting concentrations of DPPH and ABTS were 0.76 mg/ml and 0.64 mg/ml, respectively. In this study, the optimum extraction process of polysaccharide from the aerial parts of Potentilla anserina L. obtained by response surface method is convenient and feasible, and the polysaccharide has strong antioxidant activity.

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备注/Memo

备注/Memo:
收稿日期:2019-12-31基金项目:国家自然科学基金项目(31660425);青海省科技成果转化项目(2017-NK-148);青海省自然科学基金项目(2019-ZJ-978Q);青海民族大学2019年度校级理工自然科学项目 (2019XJG08)作者简介:石子林(1996-),男,湖北黄石人,硕士研究生,主要从事植物资源开发与利用研究。(E-mail)2424632465@qq.com通讯作者:李军乔,(E-mail)ljqlily2002@126.com
更新日期/Last Update: 2020-07-14