[1]李梁,叶君,李娟,等.油莎豆种质资源光合特性分析[J].江苏农业学报,2024,(09):1594-1606.[doi:doi:10.3969/j.issn.1000-4440.2024.09.003]
　LI Liang,YE Jun,LI Juan,et al.Analysis on photosynthetic characteristics of Cyperus esculentus L. germplasm resources[J].,2024,(09):1594-1606.[doi:doi:10.3969/j.issn.1000-4440.2024.09.003]
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油莎豆种质资源光合特性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年09期
页码:
1594-1606
栏目:
遗传育种·生理生化
出版日期:
2024-09-30

文章信息/Info

Title:
Analysis on photosynthetic characteristics of Cyperus esculentus L. germplasm resources
作者:
李梁1; 叶君1; 2; 3; 李娟1; 3; 路战远1; 2; 3; 李存东3; 刘文景1; 张德健1; 赵小庆1; 2
(1.内蒙古大学生命科学学院，内蒙古呼和浩特010021；2.内蒙古自治区农牧业科学院，内蒙古呼和浩特010031；3.河北农业大学农学院，河北保定071001)
Author(s):
LI Liang1; YE Jun1; 2; 3; LI Juan1; 3; LU Zhanyuan1; 2; 3; LI Cundong3; LIU Wenjing1; ZHANG Dejian1; ZHAO Xiaoqing1; 2
(1.School of Life Sciences, Inner Mongolia University, Hohhot 010021, China;2.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China;3.College of Agronomy, Hebei Agricultural University, Baoding 071001, China）
关键词:
油莎豆; 种质资源; 气体交换参数; 叶绿素荧光参数
Keywords:
Cyperus esculentus L.; germplasm resources; gas exchange parameters; chlorophyll fluorescence parameters
分类号:
S565.9;Q945.11
DOI:
doi:10.3969/j.issn.1000-4440.2024.09.003
文献标志码:
A
摘要:
为鉴选油莎豆高光效优异种质，以40份油莎豆种质资源为试验材料，测定油莎豆叶片气体交换参数、叶绿素荧光参数以及叶绿素含量等光合参数，利用相关性分析和主成分分析等多种统计学方法，综合评价与分析油莎豆种质资源光合特性。结果表明，20个光合参数的变异系数为1.23%～48.51%，总性能指数(PItotal，光系统Ⅱ、光系统Ⅰ和系统间电子传递链的整体功能活性)的变异系数最大。PItotal与Fv/Fm(t=0时，原初光化学反应最大量子产率)、Sm(受体库容量)、REo/RC(单个活性反应中心传递至光合系统Ⅰ末端电子受体使其还原的电子通量)、psi(Eo)[开始照明时，由活性反应中心捕获的单个激子驱动除还原辅酶A(QA)外的电子传递的效率]、phi(Eo)(开始照明时，还原辅酶A至除还原辅酶A外的电子传递链电子受体电子传递的量子效率)、delta(Ro) (开始照明时，由活性反应中心捕获的单个激子驱动单个电子由QA经电子传递链至PSⅠ受体侧末端电子受体的效率)、phi(Ro) (光系统Ⅰ受体侧末端电子受体还原的量子效率)、REo/CSm(t=tFm，单个活性反应中心传递至PSI末端电子受体使其还原的能量通量)、PIabs(以吸收光能为基础的性能参数)呈现显著正相关，与ABS/RC(单位反应中心吸收的光能)、DIo/RC(单个活性反应中心耗散的总能量)、TRo/RC(单位反应中心捕获的用于还原辅酶A的能量)呈现极显著负相关。主成分最小数据组分析得出，PItotal、ETo/RC(单位反应中心传递的能量)、ETo/CSm（达到荧光测量最大值时，单位叶面积用于电子传递的能量)、净光合速率(Pn)和气孔导度(Gs)可作为油莎豆高光效种质资源鉴选的重要指标。聚类分析结果表明，40份种质资源可划分为3类。其中，第Ⅰ类群有16份种质，主要表现为Pn、Gs显著高于其他2个类群。第Ⅱ类群有13份种质，主要表现为PItotal显著高于其他2个类群，第Ⅰ类群和第Ⅱ类群的ETo/RC、ETo/CSm显著高于第Ⅲ类群。第Ⅲ类群有11份种质，各参数均低于其他2个类群。不同粒型种质间差异分析结果显示，圆粒型种质的ETo/RC极显著高于长粒型种质，而长粒型种质综合得分显著高于圆粒型种质。本研究模型评价结果表明，XJ8、JL7、HE9综合表现较好，可作为高光效资源材料，为油莎豆种质资源的进一步创新和利用提供重要的参考。
Abstract:
In order to select excellent germplasm with high photosynthetic efficiency from Cyperus esculentus L., 40 C. esculentus L. germplasm resources were used as experimental materials to measure photosynthetic parameters such as gas exchange parameters, chlorophyll fluorescence parameters, and chlorophyll content in C. esculentus L. leaves. Multiple statistical methods such as correlation analysis and principal component analysis were used to comprehensively evaluate and analyze the photosynthetic characteristics of C. esculentus L. germplasm resources. The results showed that the coefficients of variation of 20 photosynthetic parameters ranged from 1.23% to 48.51%. The highest coefficient of variation was found in PItotal(the overall functional activity of photosynthetic system Ⅱ, photosynthetic system Ⅰ and electron transport chain between systems). PItotal was in significant positive correlation with Fv/Fm(when t=0, the maximum quantum yield of the initial photochemical reaction was obtained), Sm (receptor library capacity), REo/RC (the flux of electrons from a single active reaction center to the electron acceptor at the Ⅰ end of the photosynthetic system for reduction), psi(Eo) (efficiency of electron transfer driven by a single exciton, except for the reductive coenzyme A (QA)), phi(Eo) (at the start of illumination, the quantum efficiency of electron transferred from reductive coenzyme A to the electron acceptors of the respiratory chain other than reductive enzyme A), delta(Ro) (efficiency of a single exciton captured by an active reaction center to drive a single electron from the QA electron transport chain to the end of the electron acceptor on the PSI receptor side at the start of illumination), phi(Ro) (quantum efficiency of electron acceptor reduction at the end of photosystem Ⅰ receptor), REo/CSm(t=tFm, the energy flux from a single active reaction center to the electron acceptor at the end of the PSⅠ to reduce it), PIabs(performance parameters based on absorption of light energy), and was in highly significant negative correlation with ABS/RC (light energy absorbed per reaction center), DIo/RC (the total energy dissipated by a single active reaction center), and TRo/RC (energy captured by a unit reaction center for the reduction of coenzyme A). The principal component minimum data set analysis showed that PItotal, ETo/RC (energy transferred per reaction center), ETo/CSm(t=tFm, the amount of energy used for electron transfer per unit leaf area), net photosynthetic rate (Pn), and stomatal conductance (Gs) could be important indicators for the selection of high photosynthetic efficiency germplasm resources in C. esculentus L.. Results of cluster analysis showed that 40 germplasm resources could be divided into three categories. Among them, there were 16 germplasms in group Ⅰ, mainly manifested as Pn and Gs significantly higher than the other two categories. There were 13 gemplasms in group Ⅱ, mainly manifested as PItotal significantly higher than the other two categories, ETo/RC and ETo/CSm of the group Ⅰ and group Ⅱ were significantly higher than those of group Ⅲ. The group Ⅲ had 11 germplasms, and all parameters were lower than the other two groups. Analysis of differences between germplasms with different grain types showed that the ETo/RC of round grain type germplasm was significantly higher than that of long grain type germplasm, while the comprehensive score of long grain type germplasm was significantly higher than that of round grain type germplasm. The evaluation results of this research model shows that XJ8, JL7, and HE9 have good comprehensive performance and can be used as high light efficiency resource materials, which can provide important references for further innovation and utilization of C. esculentus L. germplasm resources.

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备注/Memo

备注/Memo:
收稿日期:2023-11-10基金项目:内蒙古“草原英才”工程领军人才项目；内蒙古自治区 2022 年科技领军人才团队项目(2022LJRC0010)；内蒙古农牧业创新基金项目(2022CXJJN05)；内蒙古自治区自然科学基金项目（2024MS03007）作者简介:李梁(1998-)，男，山东阳信人， 硕士研究生，研究方向为植物学。(E-mail)2806424860@qq.com。叶君为共同第一作者。通讯作者:路战远，(E-mail)lzhy281@163.com；李娟，(E-mail)lijuan198644@163.com
更新日期/Last Update: 2024-11-17