[1]胡振民,万青,李欢,等.茶树CsNRT1.1基因密码子使用特性分析[J].江苏农业学报,2019,(04):896-903.[doi:doi:10.3969/j.issn.1000-4440.2019.04.021]
　HU Zhen min,WAN Qing,LI Huan,et al.Analysis of codon usage features of CsNRT1.1 gene in Camellia sinensis[J].,2019,(04):896-903.[doi:doi:10.3969/j.issn.1000-4440.2019.04.021]
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茶树CsNRT1.1基因密码子使用特性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年04期
页码:
896-903
栏目:
园艺
出版日期:
2019-08-31

文章信息/Info

Title:
Analysis of codon usage features of CsNRT1.1 gene in Camellia sinensis
作者:
胡振民1; 万青1; 李欢1; 李荣林1; 王枫2; 杨亦扬1
（1.江苏省农业科学院休闲农业研究所，江苏省高效园艺作物遗传改良重点实验室，江苏南京210014；2.南京农业大学园艺学院，作物遗传与种质创新国家重点实验室，江苏南京210095）
Author(s):
HU Zhenmin1; WAN Qing1; LI Huan1; LI Ronglin1; WANG Feng2; YANG Yiyang1
(1.Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China; 2.College of Horticulture, Nanjing Agricultural University, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China)
关键词:
茶树; 密码子; 偏好性; 硝态氮; CsNRT1.1基因
Keywords:
Camellia sinensis;  codon;  preference;  nitrate nitrogen;  CsNRT1.1 gene
分类号:
S571101
DOI:
doi:10.3969/j.issn.1000-4440.2019.04.021
文献标志码:
A
摘要:
运用CUSP、Condow等程序对茶树CsNRT1.1基因、茶树基因组及不同物种NRT1.1基因进行密码子使用特性分析，并比较了CsNRT1.1基因与不同模式物种密码子使用偏好性差异。结果显示，茶树CsNRT1.1基因编码区偏好使用A/U，密码子结尾偏好使用G/C，RSCU值>1的偏好性密码子有26个，其中偏好性较强的只有AGG（RSCU>2），并偏好以G/C结尾；CsNRT1.1基因和茶树基因组中密码子偏好性存在差异，共计25个密码子使用偏好性存在明显差异，5个氨基酸密码子偏好性完全一致；CsNRT1.1等少数NRT1.1基因的ENc值较高，CAI、CBI值较低，说明其密码子使用偏好性较低，同时基因表达水平可能较低；CsNRT1.1基因与双子叶植物拟南芥、烟草及酵母菌密码子偏好性差异较小；对NRT1.1基因基于编码基因序列（CDS）组成和基于密码子偏性的聚类分析结果存在一定差异，但在一定程度上都能反映物种间的亲缘关系。
Abstract:
The codon usage characteristics of CsNRT1.1 gene, tea plant(Camellia sinensis) genome and NRT1.1 gene of different species were analyzed by CUSP and Condow programs, and the codon preference differences between CsNRT1.1 gene and different model species were also compared. The results showed that the CsNRT1.1 gene coding region and codon end of tea plant preferred A/U and G/C respectively, there were 26 preferred codons with RSCU value>1, among which AGG (RSCU > 2) was strongly preferred. There were differences in codon preference between CsNRT1.1 gene and tea plant genome, and a total of 25 codon usage preferences were obviously different, five amino acids had the same codon preference. A few NRT1.1 genes including CsNRT1.1 had higher ENc, lower CAI and CBI values, indicating lower codon usage preference and lower gene expression level. The difference of codon preference between CsNRT1.1 gene with dicotyledonous Arabidopsis thaliana, tobacco and yeast was relatively small. Cluster analysis results of NRT1.1 gene based on CDS composition and codon preference showed that there were some differences, but these results could reflect the relationship between species to a certain extent.

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备注/Memo

备注/Memo:
收稿日期：2018-11-30 基金项目：国家自然科学基金项目(31400587、31600558、31800590)；江苏省自然科学基金项目（BK20160590）；江苏省农业科技自主创新基金项目[CX(17)2018] 作者简介：胡振民（1986-），男，山东临沂人，博士，助理研究员，主要从事茶树栽培与茶园土壤养分管理研究。（Tel）025-84391693；（E-mail）hzm861@sina.com 通讯作者：杨亦扬，（Tel）02584391693；（E-mail）yangyiyang_yyy@126.com
更新日期/Last Update: 2019-08-31