[1]李春雷,倪德江.氟对幼龄茶树叶绿素含量及抗氧化酶活性的影响[J].江苏农业学报,2015,(05):1149-1153.[doi:doi:10.3969/j.issn.1000-4440.2015.05.032]
　LI Chun-lei,NI De-jiang.Chlorophyll content and antioxidation of young tea plant exposed to fluoride[J].,2015,(05):1149-1153.[doi:doi:10.3969/j.issn.1000-4440.2015.05.032]
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氟对幼龄茶树叶绿素含量及抗氧化酶活性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1149-1153
栏目:
园艺
出版日期:
2015-10-31

文章信息/Info

Title:
Chlorophyll content and antioxidation of young tea plant exposed to fluoride
作者:
李春雷12; 倪德江2
（1.潍坊科技学院，山东寿光262700；2.华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室，湖北武汉430070）
Author(s):
LI Chun-lei12; NI De-jiang2
（1.Weifang University of Science & Technology, Shouguang 262700, China；2.College of Horticulture and Forestry Science, Huazhong Agricultural University／Key Laboratory of Horticultural Plant Biology, Wuhan 430070, China）
关键词:
茶树; 氟; 叶绿素; 类胡萝卜素; 抗氧化酶
Keywords:
Camellia sinensis (L.); fluoride; chlorophyll content; carotenoid; antioxidative enzyme
分类号:
S571.1
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.032
文献标志码:
A
摘要:
为了探讨氟对茶树生理的影响，本试验以1年生福鼎大白茶树为试验材料，采用水培法，进行氟处理（F=16 mg/L），研究氟对茶树叶片叶绿素、类胡萝卜素含量及保护性酶的影响。结果显示，随着处理时间的延长，茶树叶片中氟含量显著升高；氟处理下叶绿素a、叶绿素b及总叶绿素含量均低于对照，叶绿素a/b显著高于对照，氟对叶绿素b的影响大于叶绿素a，氟对类胡萝卜素含量影响不显著。氟处理后，茶树叶片中超氧化物歧化酶（SOD）活性显著下降，氟处理72 h时比对照下降22.8%，比氟处理0 h时下降24.48%；氟处理后，茶树叶片中过氧化物酶（POD）和过氧化氢酶（CAT）均是先升高后降低，均是在氟处理48 h达到最大值，其中POD活性分别比0 h及对照48 h时增大31.47%和46.51%， CAT活性分别比氟处理0 h及对照48 h时增大36.79%和30.17%；MDA和H2O2含量随着处理时间的延长变化不大。
Abstract:
One-year-old cuttings ofCamellia sinensis (L.) O. Kuntze cv. Fudingdabai were grown hydroponically in order to study the effects of fluoride (F=16mg/L)on chlorophyll content, carotenoid content and antioxidative enzymes in tea plant. The results showed that the content of leaf fluoride was significantly increased over time . Under fluoride stress，chlorophyll a，chlorophyll b and total chlorophyll were lower while the ratio of chl.a to chl.b was higher than those in control， indicating that fluoride had a more significant effect on chl. b than on chl. a. The carotenoid contents changed little. Superoxide dismutase (SOD) activity was reduced by 22.80% 72 h post treatment compared with control and was reduced by 24.48% after 72-h treatment. Peroxidase (POD) and catalase (CAT) activities reached their maximum at 48 h and declined afterwards. CAT activity was 36.79% and 30.17% higher than that at the beginning of the treatment and that of CK 48 h post treatment. The contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) were kept stable over time POD activity was 31.47% and 46.51% higher than that at the beginning of the treatment and that of CK 48 h posttreatment.

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备注/Memo

备注/Memo:
收稿日期：2015-01-22 基金项目：国家自然科学基金项目（31400585）；潍坊市科技发展计划项目（201301151） 作者简介：李春雷（1979-），男，博士，副教授，主要从事茶树栽培生理与生化方面的研究。（E-mail）ligreentea@aliyun.com 通讯作者：倪德江，（E-mail）nidj2009@gmail.com
更新日期/Last Update: 2015-10-31