[1]李春雷.氟对茶树抗坏血酸?谷胱甘肽循环系统的影响[J].江苏农业学报,2016,(05):1018-1022.[doi:10.3969/j.issn.1000-4440.2016.05.010]
　LI Chun-lei.ASA-GSH cycle in tea plant exposed to fluoride application[J].,2016,(05):1018-1022.[doi:10.3969/j.issn.1000-4440.2016.05.010]
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氟对茶树抗坏血酸?谷胱甘肽循环系统的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年05期
页码:
1018-1022
栏目:
遗传育种·生理生化
出版日期:
2016-11-22

文章信息/Info

Title:
ASA-GSH cycle in tea plant exposed to fluoride application
作者:
李春雷12
1.潍坊科技学院贾思勰农学院，山东寿光262700；2. 华中农业大学园艺林学学院，湖北武汉430070
Author(s):
LI Chun-lei12
1. Jiasixie College of Agronomy, Weifang University of Science & Technology, Shouguang 262700, China;2. College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China
关键词:
茶树; 氟; 活性氧; 抗坏血酸-谷胱甘肽循环
Keywords:
Camellia sinensis (L.); fluoride;  reactive oxygen species (ROS); ascorbate-glutathione cycle
分类号:
S571.101
DOI:
10.3969/j.issn.1000-4440.2016.05.010
文献标志码:
A
摘要:
以一年生福鼎大白扦插苗为材料，采用水培法，研究不同浓度氟对茶树抗坏血酸-谷胱甘肽(ASA-GSH)循环中酶及抗氧化物质的影响。结果表明：随着氟处理浓度的增加，抗坏血酸过氧化物酶(APX) 、谷胱甘肽还原酶（GR）、单脱氢抗坏血酸还原酶（MDHAR）活性均先升后降；总抗坏血酸（T-ASA）、还原型抗坏血酸（ASA）、脱氢抗坏血酸（DHA）含量显著增加；脱氢抗坏血酸还原酶（DHAR）活性、总谷胱甘肽（T-GSH）含量、还原型谷胱甘肽（GSH）含量均呈下降趋势；ASA/DHA和GSH/GSSG均显著下降；丙二醛（MDA）、过氧化氢（H2O2）含量显著增加。说明，在低氟范围内，ASA-GSH循环对氟胁迫做出了抵御响应，及时清除活性氧（ROS），保护茶树免受伤害，但随着氟浓度的增加，ROS产生量超出了这些酶及抗氧化物质的清除能力，导致过量的ROS积累。
Abstract:
One-year-old seedlings of Camellia sinensis (L.) O. Kuntze cv. Fudingdabai were grown hydroponically in order to study the effects of fluoride (F) on the activities of relative oxidative enzymes and antioxidation substances in the ascorbate glutathione (ASA-GSH) cycle in tea plant. With increasing F concentrations, ascorbate peroxidase (APX), glutathione reductase (GR) and monodehydroascorbate reductase (MDHAR) activities increased first and decreased afterwards, ascorbate (ASA) and dehydroascorbate (DHA) contents increased significantly, dehydroascorbate reductase (DHAR) activity，total ascorbate (T-GSH) and glutathione (GSH) as well as ASA/DHA and GSH/GSSG declined, and malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents increased significantly. In conclusion, at low F concentrations, ASA-GSH cycle could scavenge reactive oxygen species (ROS) to protect cells from free radical injury. However, ROS was accumculated due to incapability of scavenging of ASA-GSH cycle in response to high F concentration.

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备注/Memo

备注/Memo:
收稿日期：2016-03-31 基金项目：国家自然科学基金项目（31400585）；潍坊市科技发展计划项目（2013YD181） 作者简介：李春雷（1979-），男，山东潍坊人，博士，副教授，主要从事茶树栽培生理与生化方面的研究。（E-mail）ligreentea@aliyun.com
更新日期/Last Update: 2016-11-22