[1]丁彬彬,张旭,吴磊,等.小麦3B 短臂染色体抗赤霉病主效 QTL 区域候选基因的表达[J].江苏农业学报,2017,(01):6-12.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
　DING Bin-bin,ZHANG Xu,WU Lei,et al.Expression of candidate genes on the region of a major QTL for the resistance to Fusarium head blight on the short arm of chromosome 3B in wheat[J].,2017,(01):6-12.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
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小麦3B 短臂染色体抗赤霉病主效 QTL 区域候选基因的表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
6-12
栏目:
遗传育种·生理生化
出版日期:
2017-02-28

文章信息/Info

Title:
Expression of candidate genes on the region of a major QTL for the resistance to Fusarium head blight on the short arm of chromosome 3B in wheat
作者:
丁彬彬12; 张旭2; 吴磊2; 王秀娥1; 马鸿翔2
（1. 南京农业大学作物遗传与种质创新国家重点实验室，江苏南京210095；2. 江苏省农业科学院江苏省农业生物学重点实验室/江苏省作物生产协同创新中心，江苏南京210014）
Author(s):
DING Bin-bin12; ZHANG Xu2; WU Lei2; WANG Xiu-er1; MA Hong-xiang2
（1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;2.State Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences/ Collaborative Innovation Center of Crop Production in Jiangsu Province, Nanjing 210014, China）
关键词:
小麦; 赤霉病抗性基因; 半定量PCR; 定量PCR; 基因表达
Keywords:
wheat; gene resistant to Fusarium head blight; RT-PCR; real-time PCR; gene expression
分类号:
S512.103.2
DOI:
10.3969/j.issn.1000-4440.2017.01.002
文献标志码:
A
摘要:
通过研究赤霉病菌接种后的基因表达，筛选小麦赤霉病抗性相关基因。根据前人研究结果，利用FGENESH软件对小麦3B短臂抗赤霉病主效QTL所在的小麦基因组测序获得的重叠群ctg0954核苷酸序列进行基因预测，共预测得到489个基因。以携带该QTL的苏麦3号和未携带该QTL的扬麦15为材料，在小麦花期进行赤霉病菌接种诱导基因表达，接种后0 h、4 h、8 h、12 h、24 h对其中有功能注释的259个预测基因的表达进行半定量分析，获得27个在品种间差异表达的基因。这27个基因的表达分为4类：一直上调表达（Ⅰ型）,在接种后的早期上调表达后期下调表达（Ⅱ型）,接种后早期下调表达后期上调表达（Ⅲ型）,接种后一直下调表达（Ⅳ型）。在差异表达基因中，选择6个基因，进行Real-time PCR 分析，结果表明这些基因在接种病原菌后均上调表达，且在苏麦3号和扬麦15号品种间存在基因表达差异，验证了半定量表达结果，可作为与赤霉病抗性相关3B短臂主效QTL的候选基因。
Abstract:
In order to screen the candidate gene for the resistance to wheat Fusarium head blight, the gene expression after inoculation of Fusarium pathogen was investigated. Based on previous reports, 489 genes were predicted in the ctg0954 where the major QTL for the resistance to wheat Fusarium head blight on short arm of chromosome 3B by using FGENESH software. Two wheat cultivars, Sumai 3 with the QTL and Yangmai 15 without the QTL, were selected as the material. 27 genes with different expression between Sumai 3 and Yangmai 15 among 259 functional predicted genes were obtained by RT-PCR in 0 h, 4 h, 8 h, 12 h, 24 h after Fusarium pathogen inoculation at anthesis. The differentially expressed genes in Sumai 3 in comparison with Yangmai 15 were grouped into four clusters: up regulated expression at most time (typeⅠ), up regulation expression at early time points and down regulated expression at later time points (typeⅡ), down regulated expression at early time points and up regulation expression at later points (typeⅢ), down regulated expression during all the time after inoculation (typeⅣ). Six out of 27 differentially expressed genes were selected for Real-time PCR, and the results showed that the six genes were up regulated after pathogen inoculation and had differences in gene expression between Sumai 3 and Yangmai 15, which might be as the candidate genes of major QTL on the short arm of chromosome 3B associated with the resistance to Fusarium head blight for further research.

参考文献/References:

[1]马鸿翔,陆维忠. 小麦赤霉病抗性改良研究进展[J]. 江苏农业学报,2010,26(1):197-203.
[2]康振生.我国植物真菌病害的研究现状及发展策略[J]. 植物保护, 2010, 36(3):9-12.
[3]段成鼎，任兰柱，范建芝，等. 种子包衣结合扬花期喷雾对小麦赤霉病防治效果研究[J]. 山东农业科学，2015，47(6):104-106.
[4]BUERSTMAYR H, BAN T, ANDERSON J A. QTL mapping and marker-assisted selection for Fusarium, head blight resistance in wheat: a review [J]. Plant Breeding, 2009, 128(1):1-26.
[5]LI T, BAI G, WU S, et al. Quantitative trait loci for resistance toFusarium head blight in the Chinese wheat landrace Huangfangzhu [J].Euphytica, 2012, 185:93-102.
[6]WALDRON B L, MALDRON-SEVILLA B, ANDEERSON J A. RFLP mapping of QTL for Fusarium head blight resistance in wheat[J]. Crop Science, 1999, 39: 805-811.
[7]ANDERSON J A, STACK R W, LIU S, et al. DNA markers for Fusarium head blight resistance QTLs in two wheat populations [J]. Theoretical & Applied Genetics, 2001, 102(8):1164-1168.
[8]BAI G, KOLB F L, SHANER G, et al.Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat [J]. Phytopathology, 1999, 89(4):343-348.
[9]ZHOU W, KOLB F L, BAI G, et al. Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers[J]. Genome, 2002, 45(4):719-727.
[10]BUERSTMAYR H, LEMMENS M, HARTL L, et al. Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance) [J]. Theoretical & Applied Genetics, 2002, 104(1):84-91.
[11]BUERSTMAYR H, STEINER B, HARTL L, et al.Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread [J]. Theoretical & Applied Genetics, 2003, 107(3):503-508.
[12]ZHANG X, ZHOU M, REN L, et al. Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai [J]. Euphytica, 2004, 139(1):59-64.
[13]周淼平,任丽娟,张旭,等. 3B染色体短臂小麦赤霉病抗性主效QTL的分析[J]. 遗传学报, 2003, 30(6):571-576.
[14]YANG Z, GILBERT J, FEDAK G, et al. Genetic characterization of QTL associated with resistance toFusarium head blight in a doubled-haploid spring wheat population[J]. Genome, 2005, 48(2):187-196.
[15]LIU S, ANDERSON J A. Targeted molecular mapping of a major wheat QTL for Fusarium head blight using ESTs and synteny with rice [J]. Genome, 2003, 46:817-823.
[16]LIU S, ZHANG X, PUMPHREY M O, et al.Complex microcolinearity among wheat, rice, and barley revealed by fine mapping of the genomic region harboring a major QTL for resistance to Fusarium head blight in wheat [J]. Functional & Integrative Genomics, 2006, 6(2):83-89.
[17]YU B Y, BAI G H, CAI S B, et al. New Fusarium head blightresistant sources from Asian wheat germplasm[J]. Crop Science, 2008, 48: 1090-1097.
[18]ZHOU M P, HAYDEN M J, ZHANG Z Y, et al.Saturation and mapping of a major Fusarium head blight resistance QTL on chromosome 3BS of Sumai 3 wheat [J]. Journal of Applied Genetics, 2010, 51(1):19-25.
[19]LIU S, PUMPHREY M O, GILL B S, et al. Toward positional cloning of Fhb1, a major QTL forFusarium head blight resistance in wheat[J]. Cereal Research Communications, 2008, 36(1):195-201. [20]BRENCHLEY R，SPANNAGL M，PFEIFER M，et al. Analysis of the bread wheat genome using whole-genome shotgun sequencing[J]. Nature，2012，491( 7426):705-710．
[21]PAUX E，SOURDILLE P, SALSE J, et al. A physical map of the 1-gigabase bread wheat chromosome 3B[J]. Science，2008，322(5898): 101-104.
[22]HAO C Y, PERRETANT M R, CHOULET F, et al.Genetic diversity and linkage disequilibrium studies on a 3.1-Mb genomic region of chromosome 3B in European and Asian bread wheat (Triticum aestivum L.) populations [J]. Theor Appl Genet , 2010, 121: 1209-1225.
[23]CUTHBERT P A, SOMERS D J, THOMAS J, et al. Fine mapping Fhb1, a major gene controllingFusarium head blight resistance in bread wheat (Triticum aestivum L.) [J]. Theor Appl Genet, 2006, 112(8):1465-1472.
[24]余桂红. 小麦赤霉病抗性遗传分析及分子标记的开发[D].上海:上海交通大学, 2008.
[25]BERNARDO A N, MA H, ZHANG D, et al.Single nucleotide polymorphism in wheat chromosome region harboring Fhb1 for Fusarium head blight resistance[J]. Molecular Breeding, 2012, 29(2):477-488.
[26]HAO C, WANG Y, HOU J, et al.Association mapping and haplotype analysis of a 3.1-Mb genomic region involved in Fusarium head blight resistance on wheat chromosome 3BS[J]. Plos One, 2012, 7(10):e46444.
[27]HILLAMBROZ K, WEBB C A, MATTHEWS A R, et al. Expressionanalysis and physical mapping of a cDNA library of Fusarium head blight infected wheat spikes[J]. Crop Science, 2006, 46(6):15.
[28]KONG L, ANDERSON J M, OHM H W. Induction of wheat defense and stress-related genes in response to Fusarium graminearum [J]. Genome, 2005, 48(1):29-40.
[29]BERNARDO A, BAI G, GUO P, et al.Fusarium graminearum-induced changes in gene expression between Fusarium head blight-resistant and susceptible wheat cultivars[J]. Functional & Integrative Genomics, 2007, 7(1):69-77. [30]STEINER B, KURZ H, LEMMENS M, et al. Differential gene expression of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation[J]. Theoretical & Applied Genetics, 2009, 118(4):753-764.
[31]MOORE J W, LOAKE G J, SPOEL S H. Transcription dynamics in plant immunity [J]. Plant Cell, 2011, 23(8):2809-2820.
[32]WANG L, TSUDA K, SATO M, et al. Arabidopsis CaM binding protein CBP60g contributes to MAMP-induced SA accumulation and is involved in disease resistance against Pseudomonas syringae [J]. Plos Pathogens, 2009, 5(2):717-721.
[33]马信. 小麦抗赤霉病相关基因的克隆及功能分析[D].泰安:山东农业大学,2014.

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备注/Memo

备注/Memo:
收稿日期：2016-03-10 基金项目：国家自然科学基金项目（31561143004）；江苏省科技支撑计划（农业）重点项目（BE2015352、BE2013439）；江苏省农业科技自主创新基金项目[CX(15)1001]；国家小麦产业技术体系项目（CARS-03）；江苏省“333”人才工程项目（BRA2014314） 作者简介：丁彬彬（1987-），男，江苏徐州人，硕士研究生，研究方向为作物遗传育种。（Tel）15150530830;(E-mail)dingbin1224@126.com 通讯作者：马鸿翔,（Tel）025-84390300;(E-mail)hxma@jaas.ac.cn
更新日期/Last Update: 2017-04-12