[1]孙大元,周丹华,张景欣,等.广谱抗源H4 中2 个主效抗病基因的单基因系构建及评价[J].江苏农业学报,2017,(01):1-5.[doi:10.3969/j.issn.1000-4440.2017.01.001 ]
　SUN Da-yuan,ZHOU Dan-hua,ZHANG Jing-xin,et al.Development of near-isogenic lines for two blast resistance genes in an indicarice H4[J].,2017,(01):1-5.[doi:10.3969/j.issn.1000-4440.2017.01.001 ]
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广谱抗源H4 中2 个主效抗病基因的单基因系构建及评价()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
1-5
栏目:
遗传育种·生理生化
出版日期:
2017-02-28

文章信息/Info

Title:
Development of near-isogenic lines for two blast resistance genes in an indicarice H4
作者:
孙大元1; 周丹华2; 张景欣1; 王慧2; 朱小源1; 杨祁云1; 陈志强2
１. 广东省农业科学院植物保护研究所/ 广东省植物保护新技术重点实验室，广东广州５１０６４０ ２. 华南农业大学国家植物航天育种工程技术研究中心，广东广州５１０６４２
Author(s):
SUN Da-yuan1; ZHOU Dan-hua2; ZHANG Jing-xin1; WANG Hui2; ZHU Xiao-yuan1; YANG Qi-yun1; CHEN Zhi-qiang2
(1. Plant Protection Research Institute Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640,China;2. National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642,China)
关键词:
稻瘟病抗性基因; 分子标记辅助选择; 近等基因系
Keywords:
blast resistance gene; marker-assisted selection; near-isogenic lines
分类号:
S332.2
DOI:
10.3969/j.issn.1000-4440.2017.01.001
文献标志码:
A
摘要:
近等基因系是准确鉴定病原菌无毒基因型 监测病原菌群体无毒基因结构动态变化以及开展寄主抗病性遗传研究与抗病育种应用的重要材料 为了明确广谱抗源Ｈ４ 中２ 个主效抗病基因Ｐｉｋ￣Ｈ４ 和Ｐｉｔａ￣Ｈ４ 与已知等位基因之间的抗性差异 以丽江新团黑谷( ＬＴＨ) 为背景 采用分子标记辅助选择技术和抗性评价等方法 构建广谱抗源Ｈ４ 的２ 个主效抗病基因Ｐｉｋ￣Ｈ４ 和Ｐｉｔａ￣Ｈ４ 的近等基因系 结果表明:携带Ｐｉｋ￣Ｈ４ 的近等基因系的抗性明显好于携带Ｐｉｔａ￣Ｈ４ 的近等基因系
Abstract:
Near-isogenic lines（NIL） are expected to be a valuable genetic resource for blast resistance， monitoring blast races and revealing their pathogenicities. To understand resistance difference between Pik-H4, Pita-H4 and their allelic gene, we constructed two near-isogenic lines with LTH as background by combining marker-assisted selection with resistance evaluation in this study. It was found that the resistance of NIL carrying Pik-H4 was better than that carrying Pita-H4.

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备注/Memo

备注/Memo:
收稿日期：2016-04-18 基金项目：广东省自然科学基金重点项目（2016A030311017）；“十二五”国家“863”计划项目（2012AA101201）；国家自然科学基金项目（31401722） 作者简介：孙大元（1984-），男，河南信阳人，博士，助理研究员，主要从事水稻抗病育种研究。(Tel)13533422395；(E-mail)307321118@qq.com。周丹华为共同第一作者。 通讯作者：杨祁云，(Tel)020-87544321;(E-mail)839034017@qq.com。陈志强，(Tel)020-85285772;(E-mail) chenlin@scau.edu.cn
更新日期/Last Update: 2017-04-12