[1]周淼平,姚金保,张鹏,等.小麦基部茎秆禾谷丝核菌DNA含量的定量检测[J].江苏农业学报,2015,(04):750-755.[doi:10.3969/j.issn.1000-4440.2015.04.007]
　ZHOU Miao-ping,YAO Jin-bao,ZHANG Peng,et al.Quantitative detection and application of Rhizoctonia cerealis DNA content in basal stalk of wheat[J].,2015,(04):750-755.[doi:10.3969/j.issn.1000-4440.2015.04.007]
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小麦基部茎秆禾谷丝核菌DNA含量的定量检测()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
750-755
栏目:
植物保护
出版日期:
2015-08-31

文章信息/Info

Title:
Quantitative detection and application of Rhizoctonia cerealis DNA content in basal stalk of wheat
作者:
周淼平; 姚金保; 张鹏; 杨学明; 余桂红; 马鸿翔
（江苏省农业科学院农业生物技术研究所/江苏省农业生物学重点实验室，江苏南京210014）
Author(s):
ZHOU Miao-ping; YAO Jin-bao; ZHANG Peng; YANG Xue-ming; YU Gui-hong; MA Hong-xiang
(Institute of Agro-biotechnology, Jiangsu Academy of Agricultural Sciences / Provincial Key Laboratory of Agrobiology, Nanjing 210014,China)
关键词:
小麦; 纹枯病; 荧光定量PCR方法
Keywords:
wheat; sharp eyespot; real-time PCR
分类号:
S512.1
DOI:
10.3969/j.issn.1000-4440.2015.04.007
文献标志码:
A
摘要:
为探索小麦基部茎秆中禾谷丝核菌DNA含量与其纹枯病抗性的关系，以掺入不同浓度梯度的禾谷丝核菌DNA小麦基因组DNA为模板，采用荧光定量PCR方法建立了小麦基部茎秆禾谷丝核菌DNA定量测定方法。通过该定量测定方法对42份室内接种禾谷丝核菌以及12份田间接种禾谷丝核菌小麦品种（系）的基部茎秆禾谷丝核菌DNA含量进行了测定，并与其纹枯病平均病级进行相关性分析，结果表明，室内接种的幼苗基部茎秆禾谷丝核菌DNA含量与其纹枯病平均病级数显著相关，相关系数为0.76。田间接种的成株期基部茎秆禾谷丝核菌DNA含量与其纹枯病平均病级相关性不显著。该研究结果可为今后开展小麦幼苗的纹枯病抗性研究提供参考。 
Abstract:
To explore the relationship between wheat sharp eyespot resistance and Rhizoctonia cerealis DNA content in basal stalk, wheat genomic DNA mixed with the gradient concentrations of Rhizoctonia cerealis DNA was used as template, and the quantitative determination method for R. cerealis DNA content in wheat background was developed by real-time PCR. The R. cerealis DNA contents in basal stalk of 42 varieties inoculated indoor and 12 varieties inoculated in wheat field were then measured. The correlation between R. cerealis DNA content and the average disease grade of sharp eyespot were also analyzed. The result showed that there was a significant correlation between R. cerealis DNA content and the average disease grade of sharp eyespot inoculated indoor, with the correlation coefficient of 0.76. The DNA content did not present close relationship to the disease grade in the field. The findings may provide a basis for the research of wheat seedlings resistance to sharp eyespot in the future.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期：2015-02-25 基金项目：国家现代农业产业技术体系项目（CARS-3）；江苏省自主创新专项[CX(13)2021]；国家转基因生物新品种培育重大专项（2013ZX08002001） 作者简介：周淼平（1968-），男，江苏兴化人，硕士，研究员，主要从事小麦生物技术育种研究。（Tel）025-84390298；（E-mail）mpzhou@jaas.ac.cn
更新日期/Last Update: 2015-08-31