[1]范奕,韩儆仁,张娟宁,等.马铃薯无糖组织培养的条件优化及其在马铃薯原原种生产中的应用[J].江苏农业学报,2025,(05):858-866.[doi:doi:10.3969/j.issn.1000-4440.2025.05.004]
　FAN Yi,HAN Jingren,ZHANG Juanning,et al.Optimization of sugar-free tissue culture conditions for potato and its application in pre-basic seed production[J].,2025,(05):858-866.[doi:doi:10.3969/j.issn.1000-4440.2025.05.004]
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马铃薯无糖组织培养的条件优化及其在马铃薯原原种生产中的应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年05期
页码:
858-866
栏目:
遗传育种·生理生化
出版日期:
2025-05-31

文章信息/Info

Title:
Optimization of sugar-free tissue culture conditions for potato and its application in pre-basic seed production
作者:
范奕1; 韩儆仁1; 张娟宁1; 唐勋2; 苗叶3; 阿金娟4; 李亚杰1; 马宁1
(1.定西市农业科学研究院/甘肃省马铃薯产业技术创新中心,甘肃定西743000；2.甘肃农业大学生命科学技术学院,甘肃兰州730070；3.建平县农业技术推广中心,辽宁朝阳122400；4.定西市安定区园艺工作站，甘肃定西743000)
Author(s):
FAN Yi1; HAN Jingren1; ZHANG Juanning1; TANG Xun2; MIAO Ye3; A Jinjuan4; LI Yajie1; MA Ning1
(1.Dingxi Academy of Agricultural Sciences/Gansu Province Potato Industry Technology Innovation Center, Dingxi 743000, China；2.College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China；3.Jianping County Agricultural Technology Extension Center, Chaoyang 122400, China；4.Dingxi City Anding District Horticultural Research Station, Dingxi 743000, China)
关键词:
无糖组织培养; 马铃薯; 组培苗
Keywords:
sugar-free tissue culture; potato; tissue-cultured seedlings
分类号:
Q813.1+2
DOI:
doi:10.3969/j.issn.1000-4440.2025.05.004
文献标志码:
A
摘要:
为了进一步优化无糖组织培养的微环境参数,本研究以青薯9号为供试材料,设置不同梯度蛭石与营养液配比(V蛭石∶V营养液=1.0∶0.7、V蛭石∶V营养液=1.0∶0.8、V蛭石∶V营养液=1.0∶0.9)、CO2含量(500 μmol/mol、800 μmol/mol、1 100 μmol/mol、1 400 μmol/mol)、光照度
[50 μmol/(m2·s)、75 μmol/(m2·s)、100 μmol/(m2·s)]试验,探究各处理对马铃薯组培苗生长发育的影响。研究结果表明,马铃薯无糖组织培养的最适条件为V蛭石∶V营养液=1.0∶0.8,CO2含量800 μmol/mol,光照度75 μmol/(m2·s)。与传统组织培养相比,无糖组织培养得到的马铃薯原原种的产量、数量和成活率分别显著提高16.09%、22.37%和3.27%(P＜0.05)。综上所述,本研究结果不仅明确了无糖组织培养的关键技术参数,还证实了该技术在提升马铃薯种薯生产效率方面的优势,为马铃薯组培苗的标准化生产和工厂化繁育提供了理论依据和技术支撑。
Abstract:
To further optimize the microenvironment parameters of sugar-free tissue culture, this study used Qingshu No.9 as the test material and designed different gradient experiments with different ratios of vermiculite to nutrient solution (Vvermiculite∶Vnutrient solution=1.0∶0.7, Vvermiculite∶Vnutrient solution=1.0∶0.8, Vvermiculite∶Vnutrient solution=1.0∶0.9), CO2 concentrations (500 μmol/mol, 800 μmol/mol, 1 100 μmol/mol, 1 400 μmol/mol), and light intensities to investigate the effects of each treatment on the growth and development of potato tissue-cultured seedlings. The light intensity was set at 50 μmol/(m2·s), 75 μmol/(m2·s), 100 μmol/(m2·s). The results showed that the optimal conditions for sugar-free tissue culture of potatoes were as follows: Vvermiculite∶Vnutrient solution=1.0∶0.8, CO2 concentration of 800 μmol/mol, and light intensity of 75 μmol/(m2·s). Compared with traditional tissue culture, the yield, quantity, and survival rate of potato pre-basic seeds obtained through sugar-free tissue culture were significantly increased by 16.09%, 22.37%, and 3.27%, respectively (P＜0.05). In conclusion, this study not only clarified the key technical parameters of sugar-free tissue culture but also confirmed the advantages of this technology in improving the production efficiency of potato seed tubers, providing theoretical basis and technical support for the standardized production and industrial propagation of potato tissue-cultured seedlings.

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备注/Memo

备注/Memo:
收稿日期:2025-01-13基金项目:甘肃省自然科学基金项目(23JRRJ0002)；甘肃省青年人才个人项目(2025QNGR83)；甘肃省科技重大专项计划资助项目(23ZDNA006)；定西市重点技术攻关专项(DX2023AZ13、DX2024AZ14)作者简介:范奕(1994-),女,甘肃定西人,硕士,助理研究员,主要从事马铃薯遗传育种及栽培技术研究。(E-mail)fanlly15101788930@163.com通讯作者:马宁,(E-mail)dxmaning@163.com；李亚杰,(E-mail)liyajie_2008@163.com
更新日期/Last Update: 2025-06-24