[1]徐玉伟,印敬明,白潇,等.马铃薯 StPYL1 和 StPYL8 基因的分子克隆与表达分析[J].江苏农业学报,2015,(01):23-31.[doi:10.3969/j.issn.1000-4440.2015.01.004]
　XU Yu-wei,YIN Jing-ming,BAI Xiao,et al.Molecular cloning and expression analysis of potato StPYL1 and StPYL8 genes[J].,2015,(01):23-31.[doi:10.3969/j.issn.1000-4440.2015.01.004]
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马铃薯 StPYL1 和 StPYL8 基因的分子克隆与表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
23-31
栏目:
遗传育种·耕作栽培·生理生化
出版日期:
2015-02-28

文章信息/Info

Title:
Molecular cloning and expression analysis of potato StPYL1 and StPYL8 genes
作者:
徐玉伟; 印敬明; 白潇; 史珂; 杨清
（南京农业大学生命科学学院, 江苏南京210095）
Author(s):
XU Yu-wei; YIN Jing-ming; BAI Xiao; SHI Ke; YANG Qing
（College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China）
关键词:
马铃薯; StPYL1; StPYL8; 克隆; 表达
Keywords:
potato; StPYL1; StPYL8; cloning; expression
分类号:
S532.03
DOI:
10.3969/j.issn.1000-4440.2015.01.004
文献标志码:
A
摘要:
PYL(Pyrabactin resistance like)是最新发现的一种ABA受体蛋白，在ABA信号转导过程中起着重要的作用。本研究采用RT-PCR方法从马铃薯品种Désirée中克隆StPYL1和StPYL8 cDNA序列（GenBank登录号：KJ660844、KJ660845）。StPYL1 cDNA开放阅读框长度为696 bp,编码一个由231个氨基酸残基组成的蛋白；StPYL8 cDNA开放阅读框长度为561 bp，编码一个由186个氨基酸残基组成的蛋白。结构分析显示：StPYL1蛋白含有3个α螺旋、3个β折叠；StPYL8蛋白含有2个α螺旋、4个β折叠；二者均含有START-like结构域；蛋白三级结构比较显示，StPYL1与拟南芥AtPYL1相似,StPYL8与拟南芥AtPYL9相似。系统进化树分析发现，StPYL1与苜蓿MtPYR1、StPYL8与拟南芥AtPYL8亲缘关系较近。组织表达分析结果表明，StPYL1和StPYL8在茎叶中均有表达，StPYL1在茎中表达最高，StPYL8在叶中表达强于茎。StPYL1和StPYL8在马铃薯块茎中都有所表达，但整个块茎发育阶段StPYL8表达量高于StPYL1。外源ABA处理诱导StPYL1和StPYL8上调表达，盐和干旱胁迫对StPYL8表达的影响大于对StPYL1的影响。
Abstract:
PYL(Pyrabaction resistance like) identified in recent research is a novel abscisic acid receptor and plays important roles in ABA responses. Two ABA receptor genes, designated as StPYL1 and StPYL8, were isolated from potato Désirée (Solanum tuberosum) by RT-PCR (GenBank accession numbers：KJ660844 and KJ660845). The ORF of StPYL1 is 696 bp long and encodes a putative protein of 231 amino acids. The ORF of StPYL8 is 561 bp long and encodes a putative protein of 186 amino acids. Protein secondary structure prediction analysis shows that protein StPYL1 has 3 α-helices and 3 β-strands and StPYL8 is composed of 2 α-helices and 4 β-strands. They all have a START-like domain. The 3D structure comparison indicated that StPYL1 and StPYL8 shared high similarities with AtPYL1 and AtPYL9, respectively. Evolutionary analysis revealed that StPYL1 had the nearest genetic relationship with MtPYR1 and StPYL8 had the nearest genetic relationship with AtPYL8. Analysis of mRNA level exhibited that StPYL1 and StPYL8 were expressed in the stem and leaves. StPYL1 was expressed more in stem and StPYL8 higher in leaves. The expression level of StPYL8 was greater during the whole tuberization than StPYL1. Both StPYL1 and StPYL8 genes were upregulated by exogenous ABA treatment. StPYL8 gene was upregulated under salinity and drought stresses, however StPYL1 was less affected.

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备注/Memo

备注/Memo:
收稿日期：2014-04-16 作者简介：徐玉伟（1986-），男，河南信阳人，硕士研究生，主要从事马铃薯块茎发育调控研究。(Tel)18512526695；(E-mail)xuyuwei_cn@sina.com 通讯作者：杨清，(E-mail)qyang19@njau.edu.cn
更新日期/Last Update: 2015-02-28