[1]黄双杰,曹梦珍,陈凌芝,等.氮素胁迫条件下茶树根系发育及生长素的响应[J].江苏农业学报,2023,(03):814-821.[doi:doi:10.3969/j.issn.1000-4440.2023.03.023]
　HUANG Shuang-jie,CAO Meng-zhen,CHEN Ling-zhi,et al.Auxin response and tea plant roots formation regulated by nitrogen stress[J].,2023,(03):814-821.[doi:doi:10.3969/j.issn.1000-4440.2023.03.023]
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氮素胁迫条件下茶树根系发育及生长素的响应()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年03期
页码:
814-821
栏目:
园艺
出版日期:
2023-06-30

文章信息/Info

Title:
Auxin response and tea plant roots formation regulated by nitrogen stress
作者:
黄双杰1; 曹梦珍1; 陈凌芝1; 朱润雨1; 张莉1; 匡祯超2; 孙慕芳1; 郭桂义1
(1.信阳农林学院茶学院/河南省豫南茶树资源综合开发重点实验室/河南省茶叶加工与检测工程技术研究中心，河南信阳464000；2.河南赛山悟道生态茶业科技有限公司，河南信阳464000)
Author(s):
HUANG Shuang-jie1; CAO Meng-zhen1; CHEN Ling-zhi1; ZHU Run-yu1;  ZHANG Li1; KUANG Zhen-chao2; SUN Mu-fang1; GUO Gui-yi1
(1.College of Tea Science, Xinyang Agriculture and Forestry University/Henan Provincial Key Laboratory of Tea Plant Comprehensive Utilization in South Henan/Henan Provincial Engineering Technology Research Center of Tea Processing and Testing, Xinyang 464000, China;2.Henan Saishan Wudao Ecological Tea Industry Technology Co., Ltd.， Xinyang 464000, China)
关键词:
茶树; 氮浓度; 根系; 生长素
Keywords:
Camellia sinensis; nitrogen concentration; root; auxin
分类号:
S571.1
DOI:
doi:10.3969/j.issn.1000-4440.2023.03.023
文献标志码:
A
摘要:
以茶树中茶108为材料，利用营养液水培试验研究3个氮浓度（0 mmol/L, 0.2 mmol/L, 2.5 mmol/L）条件下茶树的生长表型、生物量、全氮含量、根系发育、生长素类吲哚-3-乙酸（IAA）浓度及相关基因的表达。结果表明，与正常供氮（2.5 mmol/L）相比，低氮（0.2 mmol/L）胁迫条件下茶苗根系干物质量增加，根和叶片全氮含量显著降低，平均不定根伸长，侧根密度显著降低，根、根茎结合处和叶片IAA含量显著增加；缺氮（0 mmol/L）胁迫条件下茶苗叶片和根系干物质量减少，根、茎和叶的全氮含量显著减少，侧根密度显著降低，根、根茎结合处和叶片IAA含量显著增加。qRT-PCR结果表明，低氮（0.2 mmol/L）胁迫条件下茶苗叶片生长素合成基因CsTSB、CsCYP83B1、CsNIT2和根系生长素运输基因CsLAX1、CsPILS3相对表达量显著上调，缺氮（0 mmol/L）胁迫条件下茶苗叶片生长素合成基因CsCYP83B1、CsNIT2和根系生长素运输基因CsLAX1、CsPILS3相对表达量显著上调。由此推测，低氮和缺氮胁迫下叶片生长素合成以及向根系极性运输增加是茶苗根系对氮素胁迫响应的生理机制之一。
Abstract:
The growth phenotype, biomass, total nitrogen contents, root development, concentration of indole-3-acetic acid (IAA) and auxin related genes expression were studied by hydroponic experiment of Camellia sinensis cv. Zhongcha 108, using nutrient solution containing 0 mmol/L, 0.2 mmol/L, 2.5 mmol/L nitrogen, respectively. The results showed that, compared with the C. sinensis cv. Zhongcha 108 seedlings under normal nitrogen concentration (2.5 mmol/L) treatment, when treated under low nitrogen concentration (0.2 mmol/L) stress, the dry matter weight of root system increased, the total nitrogen contents in leaves and roots reduced significantly, the average adventitious root length elongated, the density of lateral roots reduced significantly, the IAA contents in root-shoot junction and roots increased significantly. Compared with the C. sinensis cv. Zhongcha 108 seedlings under normal nitrogen concentration, under the condition of nitrogen deficiency (0 mmol/L) stress, the dry matter weight of leaves and root system of C. sinensis cv. Zhongcha 108 reduced, the total nitrogen content in the leaves, stems and roots reduced significantly, the density of lateral roots reduced significantly, the IAA contents in leaves, root-shoot junction and roots increased significantly. Results of qRT-PCR showed that, relative expression of leaf auxin synthesis related genes CsTSB, CsCYP83B1, CsNIT2 and auxin transport related genes CsLAX1, CsPILS3 in roots of C. sinensis cv. Zhongcha 108 seedlings under low nitrogen concentration (0.2 mmol/L) stress were upregulated significantly in comparison with the seedlings under normal nitrogen treatment. Under nitrogen deficiency (0 mmol/L) stress condition, relative expression of leaf auxin synthesis related genes CsCYP83B1, CsNIT2 in leaves of C. sinensis cv. Zhongcha 108 seedlings and auxin transport related genes CsLAX1, CsPILS3 in roots of C. sinensis cv. Zhongcha 108 seedlings were upregulated significantly in comparison with the seedlings under normal nitrogen treatment. It can be concluded that the increasing of synthesis and polar transport of leaf auxin from leaves down to roots in C. sinensis cv. Zhongcha 108 seedlings under nitrogen deficiency stress and low nitrogen stress is one of the physiological mechanisms of the response of seedling roots to nitrogen stresses.

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备注/Memo

备注/Memo:
收稿日期：2022-08-29 基金项目：国家重点研发计划项目（2021YFD1601103）；河南省自然科学基金项目（222300420270）；河南省科技攻关项目（212102110117）；河南省高等学校重点科研项目（20B210018）；信阳农林学院校青年基金项目（2019LG004、20200103）；信阳农林学院茶学科技创新团队项目（XNKJTD-003）;信阳农林学院科研促进教学专项课题项目（kj2021015）作者简介：黄双杰（1983-），女，河南驻马店人，博士，讲师，主要从事茶树营养与生理研究。（E-mail）1157737358@qq.com 通讯作者：孙慕芳，（E-mail）310513267@qq.com
更新日期/Last Update: 2023-07-11