[1]贺丹,曹健康,何松林,等.芍药属植物远缘杂交亲和性及其雌蕊的生理响应机制[J].江苏农业学报,2023,(03):822-830.[doi:doi:10.3969/j.issn.1000-4440.2023.03.024]
　HE Dan,CAO Jian-kang,HE Song-lin,et al.Distant hybrid compatibility of Paeonia and its physiological response mechanism of pistil[J].,2023,(03):822-830.[doi:doi:10.3969/j.issn.1000-4440.2023.03.024]
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芍药属植物远缘杂交亲和性及其雌蕊的生理响应机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年03期
页码:
822-830
栏目:
园艺
出版日期:
2023-06-30

文章信息/Info

Title:
Distant hybrid compatibility of Paeonia and its physiological response mechanism of pistil
作者:
贺丹1; 2; 曹健康1; 何松林2; 符真珠3; 华超1; 张明星1; 王政1
（1.河南农业大学风景园林与艺术学院，河南郑州450002；2.河南科技学院园艺园林学院，河南新乡453003；3.河南省农业科学院，河南郑州450002）
Author(s):
HE Dan1; 2; CAO Jian-kang1; HE Song-lin2; FU Zhen-zhu3; HUA Chao1; ZHANG Ming-xing1; WANG Zheng1
(1.College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450002, China;2.College of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China;3.Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
关键词:
芍药; 牡丹; 远缘杂交; 保护酶活性; 胼胝质; β-1; 3-葡聚糖酶活性
Keywords:
Paeonia lactiflora; Paeonia suffruticosa distant hybridization; protective enzyme activity; callose; β-1; 3-glucanase activity
分类号:
S682.1
DOI:
doi:10.3969/j.issn.1000-4440.2023.03.024
文献标志码:
A
摘要:
为探究不同芍药属植物远缘杂交的亲和性,筛选出亲和性较好的杂交组合，以芍药老来红（LLH）、蝶恋花（DLH）、鲁红（LH）、莲台（LT）、星光灿烂(XGCL)、遍地红(BDH)、Kansas、Venus和Peter Brand 9个品种为母本，以牡丹凤丹白（FDB）为父本进行远缘杂交。测定授粉后不同时间（1 h、2 h、4 h、8 h、12 h、24 h）雌蕊中的保护酶[过氧化物酶（POD）、超氧化物歧化酶（SOD）]活性、胼胝质（β-1,3-葡聚糖）含量及β-1,3-葡聚糖酶活性，并对授粉后花粉粒萌发及花粉管生长情况进行荧光显微观察。结果表明，授粉后2 h观察到花粉粒开始萌发，BDH×FDB、Kansas×FDB和Peter Brand×FDB 3个组合的花粉粒萌发相对较晚。随后在花粉管的生长过程中，胼胝质不断沉积，花粉管开始出现扭曲、缠绕等现象。授粉后24 h，LLH×FDB、LH×FDB和Kansas×FDB 3个组合可以观察到花粉管进入柱头，但未进入子房。POD、SOD活性在9个组合间及同一组合授粉后不同时间表现出显著差异，当POD、SOD活性较高时，花粉粒萌发较快且花粉管正常生长。胼胝质含量及β-1,3-葡聚糖酶活性在不同组合间差异显著，高含量的胼胝质及低活性的β-1,3-葡聚糖酶会导致花粉管出现扭曲缠绕、肿胀等异常现象。国内的芍药品种中，LLH×FDB、LH×FDB组合的亲和性较好，BDH×FDB组合的亲和性最差；国外的芍药品种中，Kansas×FDB组合的亲和性相对较好，Peter Brand×FDB组合的亲和性最差。
Abstract:
In order to explore the compatibility of distant hybridization of different Paeonia lactiflora plants and screen out the hybrid combinations with better compatibility, P. lactiflora Laolaihong (LLH), Dielianhua (DLH), Luhong (LH), Liantai (LT), Xingguangcanlan (XGCL), Biandihong (BDH), Kansas, Venus and Peter Brand were used as female parents, and P. suffruticosa Fengdanbai (FDB) was used as male parent for distant hybridization. The activities of peroxidase (POD) and superoxide dismutase (SOD), the content of callose (β-1,3-glucan) and the activity of β-1,3-glucanase were determined at different times (1 h, 2 h, 4 h, 8 h, 12 h, 24 h) in the pistil after pollination. The germination of pollen grains and the growth of pollen tubes after pollination were observed by fluorescence microscopy. The results showed that the pollen grains began to germinate at two hours after pollination, and the pollen grains of BDH×FDB, Kansas×FDB and Peter Brand×FDB germinated relatively late. Subsequently, during the growth of the pollen tubes, the callose was continuously deposited, and the pollen tubes began to twist. At 24 h after pollination, the pollen tubes could be observed to enter the stigma but not the ovary in the three combinations of LLH×FDB, LH×FDB and Kansas×FDB. Activities of POD and SOD changed significantly in nine combinations and at different times after pollination in the same combination. When the activities of POD and SOD were higher, the pollen grains germinated faster and the pollen tubes grew normally. The callose content and β-1,3-glucanase activity were significantly different among different combinations. High callose content and low activity of β-1,3-glucanase could lead to abnormal phenomena such as distortion, winding and swelling of pollen tubes. Among domestic varieties, the combinations of LLH×FDB and LH×FDB had better affinity, and BDH×FDB combination had the worst affinity. Among foreign varieties, the combination of Kansas×FDB had relatively good affinity, and the combination of Peter Brand×FDB had the worst affinity.

参考文献/References:

[1]贺丹,谢栋博,张佼蕊,等. 利用iTRAQ技术和转录组筛选芍药属远缘杂交不亲和基因[J]. 中国农业科学, 2020, 53(6): 1234-1246.
[2]杨柳慧,张建军,王琪,等. 5个芍药属伊藤杂种的倍性鉴定及核型分析[J]. 植物研究, 2017, 37(4): 535-541.
[3]DU G C, XU J G, GAO C G, et al. Effect of low storage temperature on pollen viability of fifteen herbaceous peonies[J]. Biotechnology Reports, 2019, 21(3): e00309.
[4]KAORU T, KENJI O, TAKAHIRO K, et al. The importance of reproductive barriers and the effect of allopolyploidization on crop breeding[J]. Breeding Science, 2016, 66(3):333-349.
[5]HE D, LOU X Y, HE S L, et al. Isobaric tags for relative and absolute quantitation-based quantitative proteomics analysis provides novel insights into the mechanism of cross-incompatibility between tree peony and herbaceous peony[J]. Functional Plant Biology, 2019, 46(5):417-427.
[6]贺丹,解梦珺,吕博雅,等. 牡丹与芍药的授粉亲和性表现及其生理机制分析[J]. 西北农林科技大学报(自然科学版), 2017, 45(10): 129-136.
[7]杨晓苓,杨利平,尚爱芹,等. 百合授粉亲和性与雌蕊中保护酶和激素的关系[J]. 园艺学报, 2009, 36(6): 855-860.
[8]王保成,孙万仓,范惠玲,等. 芸芥自交亲和系与自交不亲和系SOD、POD和CAT酶活性[J]. 中国油料作物学报, 2006(2): 162-165,171.
[9]吴能表,徐光德,唐于婷,等. 自交不亲和甘蓝的花粉萌发与花柱内保护酶活性变化[J]. 西南师范大学学报(自然科学版), 2004(5): 848-851.
[10]NEDUKHA O M. Callose: localization, functions, and synthesis in plant cells[J]. Cytology and Genetics, 2015, 49(1): 49-57.
[11]WANG X Q, WU Z, WANG L Q, et al. Cytological and molecular characteristics of pollen abortion in Lily with dysplastic tapetum[J]. Horticultural Plant Journal, 2019, 5(6):281-294.
[12]张庆雯,王兆昊,祁静静,等. 植物胼胝质合成酶研究进展[J]. 园艺学报, 2021, 48(4): 661-675.
[13]杜文文,王祥宁,吴丽芳,等. 亚洲百合和铁炮百合正反杂交亲和程度的差异性分析[J]. 中国农业科学, 2012,45(23): 4854-4861.
[14]王冲,雷家军,姜闯,等. 君子兰种间杂交及自交亲和性[J]. 中国农业科学, 2011, 44(18): 3822-3829.
[15]孙晓梅,尚德郁,杨宏光,等. 细叶美女樱和美女樱种间杂交亲和性的研究[J]. 北方园艺, 2007(12): 101-102.
[16]张慧. 铝诱导甜高粱根尖胼胝质积累机制的研究[D]. 长春: 吉林大学, 2014.
[17]MEIKLE P J, BONIG I, HOOGENRAAD N J, et al. The location of (1→3)-β-glucans in the walls of pollen tubes of Nicotiana alata using a (1→3)-β-glucan-specific monoclonal antibody[J]. Planta, 1991, 185(1):1-8.
[18]万凌琳. 水稻β-1,3葡聚糖苷酶基因Osg1的功能研究[D]. 武汉: 武汉大学, 2010.
[19]HIRATSUKA S, ZHANG S L, NAKAGAKA E, et al. Selective inhibition of the growth or incompatible pollen tubes by S-protein in the Japanese pear[J]. Sexual Plant Reproduction, 2001, 13(4): 209-215.
[20]雷家军,吴超. 卷丹与亚洲百合种间杂交花粉管生长的荧光观察[J]. 东北农业大学学报, 2012, 43(4): 117-120.
[21]李忠光,李江鸿,杜朝昆,等. 在单一提取系统中同时测定五种植物抗氧化酶[J]. 云南师范大学学报(自然科学版), 2002(6): 44-48.
[22]李晶,金樑,邓志刚,等. 被子植物花粉和雌蕊互作的分子基础[J].草业科学, 2014, 31(1): 161-167.
[23]LAU J Y Y, PANG C C, RAMSDEN L, et al. Stigmatic exudate in the Annonaceae: pollinator reward, pollen germination medium or extragynoecial compitum[J]. Journal of Integrative Plant Biology, 2017, 59(12):881-894.
[24]白雪,刘占德,李建军,等. 猕猴桃花后不同天数授粉效果研究[J]. 江苏农业科学, 2020, 48(7): 166-168.
[25]郭玉洁,杨会娟,曹宇,等. 山丹与几种百合的杂交及花粉管生长荧光观察[J]. 江苏农业科学, 2018, 46(14): 116-120.
[26]刘宁. 花柱和柱头的结构[J]. 生物学通报, 1998(4): 17-19.
[27]FENG H Q, LIU C, FU R, et al. LORELEI-LIKE GPI-ANCHORED PROTEINS 2/3 regulate pollen tube growth as chaperones and coreceptors for ANXUR/BUPS receptor kinases in Arabidopsis[J]. Molecular Plant, 2019, 12(12): 1612-1623.
[28]谢晓红. 植物抗氧化酶系统研究进展[J]. 化工管理, 2015(32): 99-100.
[29]宋喜贵,佘小平. 植物体内过氧化氢的产生及其生理作用[J]. 连云港师范高等专科学校学报, 2010, 27(4): 99-103.
[30]郭云,支崇远,赵宇中. 显花植物受精早期阶段的酶作用[J]. 种子, 2007(2): 45-48.
[31]刘遵春,包东娥,扈惠灵. 金光杏梅自交不亲和性的生理机制[J]. 河南科技学院学报(自然科学版), 2017, 45(1): 10-13.
[32]张雪梅,李保国,赵志磊,等. 苹果自花授粉花粉管生长和花柱保护酶活性与内源激素含量的关系[J]. 林业科学, 2009, 45(11): 20-25.
[33]律春燕,王雁,朱向涛,等. 黄牡丹与芍药组间杂交花粉与柱头识别的解剖学研究[J]. 西北植物学报, 2009, 29(10): 1988-1994.
[34]ZAVALIEV R, UEKI S, EPEL B L, et al. Biology of callose (β-1,3-glucan) turnover at plasmodesmata[J]. Protoplasma, 2011, 248(1):117-130.
[35]崔海芳,张凡,尹俊龙,等. 胼胝质沉积与花粉发育[J]. 云南农业大学学报(自然科学), 2017, 32(3): 551-557.
[36]刘林秀,曾海涛,徐皓,等. 几种植物激素对4种山茶属植物花粉萌发及花粉管生长的影响[J].中国油料作物学报, 2021, 43(4): 700-707.

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备注/Memo

备注/Memo:
收稿日期：2022-07-04 基金项目：河南省高等学校重点科研项目（23A220001）；河南农业大学2021-2022年度“百千万”社会服务基金项目（30802210）；国家自然科学基金项目（31600568、31870698）作者简介：贺丹（1983-），女，河南新乡人，博士，副教授，主要从事园林植物育种研究。(E-mail)dandan990111@163.com 通讯作者：何松林，(E-mail)hsl213@yeah.net
更新日期/Last Update: 2023-07-11