[1]王韬远,陶冶,夏德美,等.外源喷施1-甲基环丙烯（1-MCP）对干旱胁迫下芍药幼苗生长的影响及作用机理[J].江苏农业学报,2020,(02):447-454.[doi:doi:10.3969/j.issn.1000-4440.2020.02.027]
　WANG Tao-yuan,TAO Ye,XIA De-mei,et al.Effects of spraying exogenous 1-methylcyclopropene(1-MCP) on growth of Paeonia lactiflora Pall. seedlings under drought stress and its mechanism[J].,2020,(02):447-454.[doi:doi:10.3969/j.issn.1000-4440.2020.02.027]
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外源喷施1-甲基环丙烯（1-MCP）对干旱胁迫下芍药幼苗生长的影响及作用机理()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
447-454
栏目:
园艺
出版日期:
2020-04-30

文章信息/Info

Title:
Effects of spraying exogenous 1-methylcyclopropene(1-MCP) on growth of Paeonia lactiflora Pall. seedlings under drought stress and its mechanism
作者:
王韬远; 陶冶; 夏德美; 王恺; 朱志国
（芜湖职业技术学院，安徽芜湖241103）
Author(s):
WANG Tao-yuan; TAO Ye; XIA De-mei; WANG Kai; ZHU Zhi-guo
(Wuhu Institute of Technology, Wuhu 241103, China)
关键词:
1-甲基环丙烯（1-MCP）; 芍药; 干旱胁迫; 耐旱性
Keywords:
1-methylcyclopropene(1-MCP); Paeonia lactiflora Pall.; drought stress; drought tolerance
分类号:
S682.1+2
DOI:
doi:10.3969/j.issn.1000-4440.2020.02.027
文献标志码:
A
摘要:
为了提高芍药的耐旱性，本研究以芍药品种粉玉奴为试验材料，采用自然干旱胁迫方法，研究叶面喷施1-甲基环丙烯（1-MCP）对芍药幼苗耐旱性的影响及作用机理。结果表明，自然干旱胁迫20 d明显抑制了芍药幼苗的生长，旱害指数达到70.65%。外源喷施1-MCP可明显缓解干旱胁迫对芍药幼苗造成的损伤，80 g/hm2 1-MCP处理的效果最佳。干旱胁迫达到20 d时，喷施80 g/hm2 1-MCP的芍药幼苗株高、茎粗、干质量和总根长分别较自然干旱胁迫对照（CK2）提高28.17%、14.10%、14.51%和25.31%，旱害指数降低53.63%（P<0.05），叶片叶绿素含量和根系活力分别较CK2提升93.05%和132.30%，净光合速率（Pn）、气孔导度（Gs）和蒸腾速率（Tr）分别较CK2提升58.95%、84.73%和74.05%，相对电导率、丙二醛（MDA）含量、过氧化氢（H2O2）含量及超氧阴离子（O2·-）产生速率分别较CK2降低25.17%、25.47%、21.02%和22.91%。干旱胁迫达到10 d时，喷施80 g/hm2 1-MCP的芍药幼苗叶片脯氨酸、可溶性糖、可溶性蛋白质含量均达到最高，分别较CK2提升78.67%、67.62%和94.02%（P<0.05）。综上所述，外源喷施1-MCP可通过提高芍药幼苗抗氧化能力和渗透调节能力，有效降低因干旱胁迫而导致的活性氧积累和膜脂过氧化，缓解干旱胁迫损伤，提高耐旱性。
Abstract:
In order to improve the drought tolerance of Paeonia lactiflora Pall., the variety Fenyunu was used as experimental material in this study. The effects of spraying exogenous 1-methylcyclopropene(1-MCP) on the drought tolerance of Paeonia lactiflora Pall. and its mechanisms were studied by natural drought stress method. The results showed that the growth of Paeonia lactiflora Pall. seedlings was inhibited under natural drought stress for 20 days, and the drought injury index was 70.65%. Application of exogenous 1-MCP could significantly alleviate the damage of Paeonia lactiflora Pall. seedlings caused by drought stress, and the optimal concentration was 80 g/hm2. At twenty days of natural drought stress, compared with those in the treatment of natural drought stress (CK2), the plant height, stem diameter, dry weight and total root length of Paeonia lactiflora Pall. seedlings in the treatment of spraying 80 g/hm2 1-MCP were increased by 28.17%, 14.10%, 14.51% and 25.31% respectively, the drought injury index was decreased by 53.63%(P<0.05), the chlorophyll content, root activity, net photosynthetic rate, stomatal conductance, transpiration rate were increased by 93.05%, 132.30%, 58.95%, 84.73%, 74.05%, the relative conductivity, malondialdehyde(MDA) content, hydrogen peroxide(H2O2) content and superoxide anion(O·-2) production rate were decreased by 25.17%, 25.47%, 21.02% and 22.91%. At ten days of drought stress, proline content, soluble sugar content, soluble protein content of Paeonia lactiflora Pall. seedlings in the treatment of spraying 80 g/hm2 1-MCP were increased by 78.67%, 67.62% and 94.02% compared with those in the treatment of CK2. In conclusion, exogenous spraying 1-MCP could effectively reduce the accumulation of active oxygen and the peroxidation of membrane lipid under drought stress, alleviate the injury caused by drought stress and improve the drought tolerance by improving the antioxidant capacity and osmotic regulation capacity of Paeonia lactiflora Pall. seedlings.

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备注/Memo

备注/Memo:
收稿日期：2019-08-27基金项目：芜湖职业技术学院校级自然重点基金项目（Wzyzrzd201906）；安徽省自然科学基金项目（KJ2019A0981）；芜湖职业技术学院院级科技创新团队基金项目（Wzykj2018ZX01）作者简介：王韬远（1987-），男，黑龙江哈尔滨人，硕士，讲师，主要从事园林植物遗传育种及生理生态研究。(Tel)18375328619；(E-mail)wangty@whit.edu.cn通讯作者：朱志国，(E-mail)zhuzg@whit.edu.cn
更新日期/Last Update: 2020-05-18