[1]惠小涵,程婷婷,柯卫东,等.莲藕PPO基因密码子偏好性特征分析[J].江苏农业学报,2020,(02):438-446.[doi:doi:10.3969/j.issn.1000-4440.2020.02.026]
　HUI Xiao-han,CHENG Ting-ting,KE Wei-dong,et al.Analysis on codon preference of PPO gene in lotus root[J].,2020,(02):438-446.[doi:doi:10.3969/j.issn.1000-4440.2020.02.026]
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莲藕PPO基因密码子偏好性特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
438-446
栏目:
园艺
出版日期:
2020-04-30

文章信息/Info

Title:
Analysis on codon preference of PPO gene in lotus root
作者:
惠小涵1; 程婷婷1; 柯卫东2; 郭宏波1
（1.西北农林科技大学化学与药学院,陕西杨凌712100；2.武汉市农业科学院蔬菜研究所，湖北武汉4300065）
Author(s):
HUI Xiao-han1; CHENG Ting-ting1; KE Wei-dong2; GUO Hong-bo1
(1.College of Chemistry and Pharmacy, Northwest A&F University, Yangling 712100, China;2.Institute of Vegetable, Wuhan Academy of Agricultural Sciences, Wuhan 430065, China)
关键词:
莲藕; PPO; 密码子; 偏好性
Keywords:
lotus root; polyphenol oxidase (PPO); codon; preference
分类号:
S645.1
DOI:
doi:10.3969/j.issn.1000-4440.2020.02.026
文献标志码:
A
摘要:
为了解莲藕多酚氧化酶（PPO）基因密码子使用偏好性、不同物种中PPO基因同源关系及异源表达受体,本研究运用 CondonW 和 EMBOSS 程序对莲藕PPO基因密码子进行分析。结果表明，莲藕PPO基因偏好性较弱但偏好于G/C结尾的密码子，其中高频密码子有 24 个（同义密码子相对使用度>1），偏好性最强的有 3 个（同义密码子相对使用度>2）。用SPSS和MEGA软件对34种单子叶和双子叶植物的 PPO 密码子进行偏性比较和聚类分析，发现不同物种PPO基因存在不同密码子偏好模式，多数单子叶植物PPO 基因密码子偏好性比双子叶植物强。PPO 密码子相关性分析结果表明，莲藕 PPO 密码子使用偏好主要受突变压力的影响。在异源转化受体选择中，大肠杆菌真核表达系统适合莲藕PPO的异源表达，拟南芥则是莲藕PPO转基因的理想受体。莲藕PPO密码子偏好模式分析为后续的PPO基因遗传转化研究提供了理论依据。
Abstract:
To explore the codon usage preference of polyphenol oxidase(PPO) gene in lotus root, the homology of PPO gene in different species and heterologous expression receptors, the codon of PPO gene was analyzed by CondonW and EMBOSS programs. The results showed that the PPO gene favored codons ending in G or C. There were 24 high-frequency codons (relative synoymous codon usage>1)， and three codons (relative synoymous codon usage>2) had the strongest preference. The PPO codon bias of 34 monocotyledonous and dicotyledonous plants was analyzed by SPSS and MEGA software. It was found that PPO genes from different species had different codon preference patterns, and most monocotyledonous PPO genes had stronger codon preference than dicotyledons. Correlation analysis of PPO codon indicated that the PPO codon usage preference in lotus root was mainly affected by mutation. In the selection of heterologous transforming receptors, eukaryotic expression system of Escherichia coli is suitable for heterologous expression of PPO in lotus root, and Arabidopsis thaliana is an ideal receptor for PPO transgene. The PPO codon preference pattern analysis of lotus root provides a theoretical basis for the subsequent study of PPO gene transformation.

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备注/Memo

备注/Memo:
收稿日期：2019-10-29基金项目：国家重点研发计划项目（2016YFD0100204-29）；国家杨凌农业高新技术产业示范区科技攻关计划项目（201SF-08）作者简介：惠小涵（1996-），女，陕西渭南人，硕士研究生，主要从事多酚氧化酶基因家族在莲藕等植物中的功能研究。（E-mail）huixh@nwsuaf.edu.cn通讯作者：郭宏波，（E-mail）hbguo@nwsuaf.edu.cn
更新日期/Last Update: 2020-05-18