[1]朱凌丽,徐建,姚协丰,等.厚皮甜瓜种质蔓枯病抗性评价与遗传多样性分析[J].江苏农业学报,2021,(02):454-464.[doi:doi:10.3969/j.issn.1000-4440.2021.02.023]
　ZHU Ling-li,XU Jian,YAO Xie-feng,et al.Identification of gummy stem blight resistance and analysis of genetic diversity in muskmelon germplasms[J].,2021,(02):454-464.[doi:doi:10.3969/j.issn.1000-4440.2021.02.023]
点击复制

厚皮甜瓜种质蔓枯病抗性评价与遗传多样性分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
454-464
栏目:
园艺
出版日期:
2021-04-30

文章信息/Info

Title:
Identification of gummy stem blight resistance and analysis of genetic diversity in muskmelon germplasms
作者:
朱凌丽1; 2; 徐建2; 姚协丰2; 徐锦华2; 李苹芳2; 柳李旺1; 羊杏平2
（1.南京农业大学园艺学院，江苏南京210095；2.江苏省农业科学院蔬菜研究所，江苏南京210014）
Author(s):
ZHU Ling-li1; 2; XU Jian2; YAO Xie-feng2; XU Jin-hua2; LI Ping-fang2; LIU Li-wang1; YANG Xing-ping2
（1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Vegetable, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China）
关键词:
厚皮甜瓜; 种质资源; 蔓枯病; 抗性评价; 遗传多样性
Keywords:
muskmelon; germplasm resources; gummy stem blight; resistance evaluation; genetic diversity
分类号:
S652
DOI:
doi:10.3969/j.issn.1000-4440.2021.02.023
文献标志码:
A
摘要:
采用苗期蔓枯病接种和单核苷酸多态性（SNP）基因型分析对120份厚皮甜瓜高代自交系进行抗性评价与遗传多样性研究。结果显示：光皮类型厚皮甜瓜中高抗、中抗种质资源占比达11.11%，其抗性水平结构优于网纹甜瓜和哈密瓜。统计分析结果显示，21个SNP位点的PIC值为0.050～0.478，其中12个中度多态SNP位点的PIC值平均为0.37，表明甜瓜种质的多态性中等。群体结构与聚类分析结果表明，124份甜瓜种质资源可分成5大类群，各类群具有一定的独立性，且类群间存在明显的差异，遗传多样性丰富。
Abstract:
The gummy stem blight (GSB) resistance evaluation and genetic diversity study of 120 high generation inbred lines of muskmelons were conducted via inoculation at seedling stage and single nucleotide polymorphism (SNP) genotype analysis. The results showed that, the proportion of germplasm resources of smoothbark muskmelons with high and medium resistance to GSB was up to 11.11%, and the structure of resistance level was better than that of netted melon and Hami melon. The statistical analysis results showed that, the polymorphism information content (PIC) value of 21 SNP loci ranged from 0.050 to 0.478, and the average PIC value of 12 moderate polymorphic SNP loci was 0.37, indicating that the polymorphism of the melon germplasms was moderate. Results of population structure and clustering analysis showed that, the 124 melon germplasms could be classified into five major groups, and each group showed certain independence. Furthermore, there were obvious differences between different groups and the genetic diversity was abundant.

参考文献/References:

[1]李田，刘海河，张彦萍，等. 外源硝酸胺对厚皮甜瓜坐果节位叶片衰老及果实产量和品质的影响[J]. 北方园艺，2018, 2018 (7): 15-21.
[2]王晓东，李国英. 哈密瓜蔓枯病菌分生孢子器诱发及室内品种抗病性测定[J]. 新疆农业科学，2004, 41 (5): 341-344.
[3]KEINATH A P, ZITTER T A. Resistance to benomyl and thiophanate-methyl in Didymella bryoniae from South Carolina and New York [J]. Plant Disease，1998, 82：479-484.
[4]张永兵，王登明，张聪，等. 甜瓜蔓枯病离体接种方法初步研究[J]. 新疆农业科学，2009, 46 (3): 521-525.
[5]张龑，王宣仓，李寐华，等. 新疆甜瓜品种资源蔓枯病抗性鉴定[J]. 新疆农业科学， 2011, 48 (10): 1841-1845.
[6]FRANTZ J D，JAHN M M．Five independent loci each control monogenic resistance to gummy stem blight in melon（Cucumis melo L.）[J]. Theoretical and Applied Genetics, 2004, 108 (6)：1033-1038.
[7]王红英，钱春桃，娄丽娜，等. 甜瓜抗蔓枯病基因 Gsb-4 的分子标记[J]．园艺学报，2012, 39 (3): 574-580.
[8]钱春桃，娄群峰，陈劲枫．我国甜瓜属蔬菜作物特异基因资源的挖掘和利用[J]. 中国蔬菜，2006(7): 30-32.
[9]姚协丰，徐锦华，李苹芳，等. 菜瓜蔓枯病苗期抗性鉴定方法比较[J]. 中国瓜菜，2017, 30 (2): 11-14.
[10]周晓慧，JOSEPH N W，李英，等. 甜瓜抗蔓枯病种质资源的筛选及RAPD分析[J]. 园艺学报，2007, 34 (5): 1201-1206.
[11]GABRIELE G, RONGHAO S, TODD C W. New sources of resistance to gummy stem blight in watermelon [J]. Crop Science， 2005, 45 (2)：582-588.
[12]WANG S, YANG J, ZHANG M. Developments of functional markers for Fom-2-mediated fusarium wilt resistance based on single nucleotide polymorphism in melon (Cucumis melo L.) [J]. Molecular Breeding, 2011, 27 (3): 385-393.
[13]LEIDA C, MOSER C, ESTERAS C, et al. Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon (Cucumis melo L.) [J]. BMC Genetics, 2015, 16 (1)：1-17.
[14]TZURI G, ZHOU X, CHAYUT N, et al. A ‘golden’ SNP in CmOr governs the fruit flesh color of melon (Cucumis melo) [J]. Plant Journal, 2015, 82 (2): 267-279.
[15]HU Z Y, DENG G C, MOU H P, et al. A re-sequencing-based ultra-dense genetic map reveals a gummy stem blight resistance-associated gene in Cucumis melo [J]. DNA Research，2018, 25 (1)：1-10.
[16]SAXENA R K, ERIC V W, UPADHYAYA H D, et al. Genetic diversity and demographic history of Cajanus spp. illustrated from genome-wide SNPs [J]. PLoS ONE, 2014, 9 (2): e88568.
[17]张学军，郭丽霞，马新力，等. 甜瓜种质资源抗霜霉病和蔓枯病苗期筛选与评价[J]. 中国瓜菜，2014, 2014 (27): 38-40.
[18]羊杏平，刘广，侯喜林，等. 西瓜核心种质枯萎病抗性与SRAP分子标记的关联分析[J]. 园艺学报，2013, 40 (7): 1298-1308.

相似文献/References:

[1]李建斌,李兆虎,王红,等.耐低温弱光结球甘蓝种质资源评价[J].江苏农业学报,2015,(03):645.[doi:10.3969/j.issn.1000-4440.2015.03.029]
　LI Jian-bin,LI Zhao-hu,WANG Hong,et al.Evaluation of cabbage germplasm tolerant to low temperature and weak light[J].,2015,(02):645.[doi:10.3969/j.issn.1000-4440.2015.03.029]
[2]蔡晓锋,葛晨辉,王小丽,等.中国菠菜育种技术研究现状及展望[J].江苏农业学报,2019,(04):996.[doi:doi:10.3969/j.issn.1000-4440.2019.04.035]
　CAI Xiao feng,GE Chen hui,WANG Xiao li,et al.Advances and perspectives in research of spinach breeding technology in China[J].,2019,(02):996.[doi:doi:10.3969/j.issn.1000-4440.2019.04.035]
[3]王治会,岳翠男,李琛,等.江西省茶树种质化学特性多样性分析与鉴定评价[J].江苏农业学报,2020,(01):172.[doi:doi:10.3969/j.issn.1000-4440.2020.01.024]
　WANG Zhi-hui,YUE Cui-nan,LI Chen,et al.Diversity analysis and evaluation of chemical characteristics of tea germplasms in Jiangxi province[J].,2020,(02):172.[doi:doi:10.3969/j.issn.1000-4440.2020.01.024]
[4]蔡瑾,杨继书,翟文玲,等.长江中下游麦区小麦低多酚氧化酶活性种质的初步鉴定[J].江苏农业学报,2021,(03):545.[doi:doi:10.3969/j.issn.1000-4440.2021.03.001]
　CAI Jin,YANG Ji-shu,ZHAI Wen-ling,et al.Preliminary identification of wheat germplasm with low polyphenol oxidase activity in the wheat region of the middle and lower reaches of Yangtze River valley[J].,2021,(02):545.[doi:doi:10.3969/j.issn.1000-4440.2021.03.001]
[5]赵洋,刘振,杨培迪,等.黄金茶种质资源生化成分的多样性分析[J].江苏农业学报,2021,(05):1285.[doi:doi:10.3969/j.issn.1000-4440.2021.05.025]
　ZHAO Yang,LIU Zhen,YANG Pei-di,et al.Diversity analysis of biochemical components in Huangjincha (Camellia sinensis) germplasm resources[J].,2021,(02):1285.[doi:doi:10.3969/j.issn.1000-4440.2021.05.025]
[6]肖熙鸥,林文秋,陈卓,等.马铃薯抗青枯病育种研究进展[J].江苏农业学报,2021,(05):1344.[doi:doi:10.3969/j.issn.1000-4440.2021.05.033]
　XIAO Xi-ou,LIN Wen-qiu,CHEN Zhuo,et al.Research advances in potato breeding for bacterial wilt resistance[J].,2021,(02):1344.[doi:doi:10.3969/j.issn.1000-4440.2021.05.033]
[7]辛佳佳,张南峰,程华萍,等.江西省地方蚕豆种质资源遗传多样性分析及优异资源挖掘[J].江苏农业学报,2022,38(01):20.[doi:doi:10.3969/j.issn.1000-4440.2022.01.003]
　XIN Jia-jia,ZHANG Nan-feng,CHENG Hua-ping,et al.Genetic diversity analysis and excellent resources mining of local broad bean germplasm resources in Jiangxi province[J].,2022,38(02):20.[doi:doi:10.3969/j.issn.1000-4440.2022.01.003]
[8]孙博文,黄耀,孙亚亭,等.远缘杂交结合胚培养法获得厚皮甜瓜阿鲁斯达令双单倍体[J].江苏农业学报,2022,38(03):775.[doi:doi:10.3969/j.issn.1000-4440.2022.03.024]
　SUN Bo-wen,HUANG Yao,SUN Ya-ting,et al.Production of doubled haploid in muskmelon Earl’s Darling by interspecific hybridization and embryo culture[J].,2022,38(02):775.[doi:doi:10.3969/j.issn.1000-4440.2022.03.024]
[9]田雪珂,钟启文,孙雪梅,等.叶用莴苣种质资源表型性状及营养品质综合评价[J].江苏农业学报,2022,38(05):1330.[doi:doi:10.3969/j.issn.1000-4440.2022.05.020]
　TIAN Xue-ke,ZHONG Qi-wen,SUN Xue-mei,et al.Comprehensive evaluation on phenotypic traits and nutritional quality of leaf lettuce germplasm resources[J].,2022,38(02):1330.[doi:doi:10.3969/j.issn.1000-4440.2022.05.020]
[10]张倩男,杨彩玲,买自珍,等.百合种质资源农艺与营养品质性状分析及综合评价[J].江苏农业学报,2024,(04):721.[doi:doi:10.3969/j.issn.1000-4440.2024.04.016]
　ZHANG Qian-nan,YANG Cai-ling,MAI Zi-zhen,et al.Analysis and comprehensive evaluation of agronomic and nutritional quality traits of lily germplasm resources[J].,2024,(02):721.[doi:doi:10.3969/j.issn.1000-4440.2024.04.016]

备注/Memo

备注/Memo:
收稿日期：2020-07-02基金项目：国家西甜瓜产业技术体系项目(CARS-26-08)；江苏省农业三新工程项目[SXGC（2017）088]；徐州市重点研发计划项目（KC18119）作者简介：朱凌丽（1981-），女，江苏盐城人，硕士，助理研究员，主要从事甜瓜新品种选育工作。（E-mail）sweetmelon2050@foxmail.com通讯作者：柳李旺，（E-mail）nauliulw@njau.edu.cn；羊杏平，（E-mail）xingping@jaas.ac.cn
更新日期/Last Update: 2021-05-10