[1]陈佳莉,代洪苇,周丽娟,等.茶树CsTLRs基因密码子偏好性与进化分析[J].江苏农业学报,2025,(05):996-1008.[doi:doi:10.3969/j.issn.1000-4440.2025.05.018]
　CHEN Jiali,DAI Hongwei,ZHOU Lijuan,et al.Codon bias and evolutionary analysis of CsTLRs in tea plant[J].,2025,(05):996-1008.[doi:doi:10.3969/j.issn.1000-4440.2025.05.018]
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茶树CsTLRs基因密码子偏好性与进化分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年05期
页码:
996-1008
栏目:
园艺
出版日期:
2025-05-31

文章信息/Info

Title:
Codon bias and evolutionary analysis of CsTLRs in tea plant
作者:
陈佳莉1; 2; 代洪苇1; 2; 周丽娟3; 童华荣1; 2; 袁连玉1; 2
(1.西南大学食品科学学院,重庆400715；2.川渝共建特色食品重庆市重点实验室,重庆400715；3.重庆北碚农业发展有限公司,重庆400715)
Author(s):
CHEN Jiali1; 2; DAI Hongwei1; 2; ZHOU Lijuan3; TONG Huarong1; 2; YUAN Lianyu1; 2
(1.College of Food Science, Southwest University, Chongqing 400715, China；2.Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing 400715, China；3.Chongqing Beibei Agricultural Development Co., Ltd., Chongqing 400715, China)
关键词:
茶树; 叶片毛状体; CsTLRs基因; 密码子偏好性; 进化分析
Keywords:
tea plant; leaf trichomes; CsTLRs genes; codon bias; evolutionary analysis
分类号:
S571.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.05.018
文献标志码:
A
摘要:
TLR基因具有调控植物毛状体生长发育的作用,而叶片毛状体(茸毛)是茶树[Camellia sinensis (L.)]的重要形态特征之一,在茶树的生理、生态和茶叶品质方面均发挥着重要作用。本研究利用CodonW、EMBOSS等软件,通过中性绘图、奇偶偏好性分析（PR2-plot）等多种分析方法,对茶树CsTLRs基因的密码子参数、亲缘关系以及异源表达受体等方面进行分析。结果表明,茶树CsTLRs基因密码子使用偏好性较弱,偏好性形成主要来源于自然选择；筛选出20个最优密码子,多以A/U结尾； 编码序列（CDS）和相对同义密码子使用度（RSCU）聚类分析结果都显示,茶树CsTLRs基因与杨树、黄瓜亲缘关系更近；烟草、拟南芥和番茄可作为CsTLRs异源转化的植物受体,酿酒酵母可作为微生物异源表达受体。综上,本研究结果明确了CsTLRs基因在调控茶树叶片毛状体发育时的密码子使用特征,为后续CsTLRs基因的功能研究提供了重要参考。
Abstract:
The TLR gene has the function of regulating the growth and development of plant trichomes. Leaf trichome is one of the important morphological characteristics of Camellia sinensis (L.), which plays an important role in the physiology and ecology of tea plant and tea quality. In this study, we conducted a comprehensive analysis of tea plant CsTLRs genes, including codon parameters, genetic relationship and heterologous expression hosts. This was achieved using multiple analytical approaches including neutral plot analysis, parity rule 2 plot (PR2-plot) analysis, implemented through softwares such as CodonW and EMBOSS. The results showed that the codon usage bias of CsTLRs genes was weak, and the bias was primarily dominated by natural selection. There were 20 optimal codons in CsTLRs genes, mostly ending with A/U. Both cluster analyses of the coding sequences (CDS) and relative synonymous codon usage (RSCU) revealed that CsTLRs genes in tea plant were phylogenetically closer to those in poplar (Populus) and cucumber (Cucumis sativus). For heterologous expression of CsTLRs genes from tea plant, tobacco (Nicotiana tabacum), Arabidopsis (Arabidopsis thaliana), and tomato (Solanum lycopersicum) could be selected as genetic transformation recipients, while the expression in microorganisms was better in Saccharomyces cerevisiae. In summary, this study analyzed the codon usage bias of CsTLRs genes in tea plants, elucidating their characteristic codon usage patterns during the regulation of leaf trichome development. These findings would provide references for future functional studies of CsTLRs genes.

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备注/Memo

备注/Memo:
收稿日期：2025-02-10基金项目：重庆市技术创新与应用发展重大专项(CSTB2022TIAD-CUX0021)；重庆市农业农村委员会特色经济作物提质增效项目(F2023757)；2024年宜宾市农业创新能力建设项目(2024NYCX012)作者简介：陈佳莉(2004-),女,重庆北碚人,本科,研究方向为茶树分子生物学。(E-mail) charli135@163.com 通讯作者：袁连玉,(E-mail) yuanlianyu@swu.edu.cn
更新日期/Last Update: 2025-06-24