[1]张斌.大豆转录因子基因GmbHLH130克隆及在干旱胁迫中的功能分析[J].江苏农业学报,2023,(07):1441-1448.[doi:doi:10.3969/j.issn.1000-4440.2023.07.001]
　ZHANG Bin.Cloning and functional analysis of soybean transcription factor GmbHLH130 gene under drought stress[J].,2023,(07):1441-1448.[doi:doi:10.3969/j.issn.1000-4440.2023.07.001]
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大豆转录因子基因GmbHLH130克隆及在干旱胁迫中的功能分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年07期
页码:
1441-1448
栏目:
遗传育种·生理生化
出版日期:
2023-10-31

文章信息/Info

Title:
Cloning and functional analysis of soybean transcription factor GmbHLH130 gene under drought stress
作者:
张斌
(湖南科技学院/湖南省银杏工程技术研究中心，湖南永州425199)
Author(s):
ZHANG Bin
(Hunan University of Science and Engineering/Hunan Provincial Engineering Research Center for Ginkgo biloba, Yongzhou 425199, China)
关键词:
大豆; GmbHLH130; 干旱胁迫; 耐旱性
Keywords:
soybean; GmbHLH130; drought stress; drought tolerance
分类号:
S565.1
DOI:
doi:10.3969/j.issn.1000-4440.2023.07.001
文献标志码:
A
摘要:
为了探究大豆GmbHLH130基因在植物干旱胁迫中的调控功能，利用生物信息学分析GmbHLH130与其他物种bHLH家族成员的系统进化关系，检测GmbHLH130基因及其启动子对干旱胁迫的响应，对GmbHLH130蛋白的亚细胞定位和转录激活活性进行分析，最后初步评估GmbHLH130基因过表达拟南芥的耐旱性。结果显示，在进化树中GmbHLH130与拟南芥AtbHLH122进化关系最近；GmbHLH130基因受干旱诱导上调表达；GmbHLH130基因启动子也受干旱诱导激活下游报告基因；烟草叶片瞬时表达分析结果表明GmbHLH130蛋白定位于细胞核，并且具有转录激活活性。此外，GmbHLH130基因过表达拟南芥在干旱处理下的绿色子叶率和根长均显著大于野生型。本研究结果初步证明了大豆GmbHLH130基因在增强植物耐旱方面的功能，为后续探究其参与耐旱性调控的分子机制提供了理论依据。
Abstract:
To explore the regulatory function of soybean GmbHLH130 gene in plant drought stress, bioinformatics was used to analyze the phylogenetic relationship between GmbHLH130 protein and bHLH members of other species. The response of GmbHLH130 and its promoter to drought stress was detected, and the subcellular localization and transcriptional activation of GmbHLH130 protein were analyzed. Finally, the drought tolerance of Arabidopsis which overexpressed GmbHLH130 was preliminarily evaluated. The results showed that GmbHLH130 protein was closest to AtbHLH122 protein in the phylogenetic tree. The expression level of GmbHLH130 gene was up-regulated under drought treatment. The promoter of GmbHLH130 gene was also induced by drought stress to activate downstream reporter gene. Transient expression of GmbHLH130 in tobacco leaves indicated that GmbHLH130 was localized in the nucleus and had transcriptional activation activity. In addition, the green cotyledon percentage and root length of GmbHLH130 overexpressed Arabidopsis were all significantly higher than those of wide type under drought treatment. The results of the study preliminarily proved the function of soybean GmbHLH130 gene in enhancing plant drought tolerance, and provided theoretical basis for further investigation of its molecular mechanism involved in drought tolerance regulation.

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备注/Memo

备注/Memo:
收稿日期：2023-01-16基金项目：湖南省自然科学基金项目(2022JJ30274)作者简介：张斌(1981-)，男，湖南永州人，博士，副教授，主要从事植物发育生物学和植物耐逆性研究。(E-mail)zhangbin27104@163.com
更新日期/Last Update: 2023-11-17