[1]张斌,陈丽娟,李其华,等.栽培大豆GRAS转录因子家族基因鉴定及其盐胁迫下表达模式分析[J].江苏农业学报,2021,(02):296-309.[doi:doi:10.3969/j.issn.1000-4440.2021.02.004]
　ZHANG Bin,CHEN Li-juan,LI Qi-hua,et al.Identification of gene of GRAS transcription factor family in cultivated soybean（Glycine max L.） and expression pattern analysis under salt stress[J].,2021,(02):296-309.[doi:doi:10.3969/j.issn.1000-4440.2021.02.004]
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栽培大豆GRAS转录因子家族基因鉴定及其盐胁迫下表达模式分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
296-309
栏目:
遗传育种·生理生化
出版日期:
2021-04-30

文章信息/Info

Title:
Identification of gene of GRAS transcription factor family in cultivated soybean（Glycine max L.） and expression pattern analysis under salt stress
作者:
张斌; 陈丽娟; 李其华; 唐满生
(湖南科技学院化学与生物工程学院,湖南省银杏工程技术研究中心,湖南永州425199)
Author(s):
ZHANG Bin; CHEN Li-juan; LI Qi-hua; TANG Man-sheng
(Hunan Provincial Engineering Research Center for Ginkgo, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, China)
关键词:
大豆; GRAS家族; 盐胁迫; 表达模式
Keywords:
soybean; GRAS family; salt stress; expression pattern
分类号:
S565.101
DOI:
doi:10.3969/j.issn.1000-4440.2021.02.004
文献标志码:
A
摘要:
利用生物信息学手段及转录组测序方法对大豆78个GRAS家族基因进行系统分析。染色体定位结果表明78个GRAS基因不均匀地分布在20条染色体上。通过系统进化分析将大豆GRAS家族分为11个亚族。基因结构和保守基序分布分析结果表明GRAS家族成员在进化上具有保守性，尤其是进化关系较近的成员多具有类似的基因结构和蛋白质结构。转录组数据及qRT-PCR结果显示，5个基因受盐胁迫诱导上调，5个基因受盐胁迫诱导下调，其中GmGRAS14、GmGRAS33和GmGRAS69在盐处理12 h时上调倍数最高，而GmGRAS17、GmGRAS54和GmGRAS57在盐处理12 h时表达量下调倍数最高，说明这些基因可能在大豆响应盐胁迫方面发挥重要功能。
Abstract:
In this study, 78 genes of GRAS family in soybean were systematically investigated using bioinformatics and RNA-seq. The chromosomal distribution map showed that 78 GRAS genes were randomly located in 20 chromosomes. Phylogenetic analysis showed that the soybean GRAS family could be divided into 11 subfamilies. The gene structure and conserved motif distribution analysis suggested that the GRAS family members were consertive in evolution, and most of the GRAS members with close evolutionary relationship had similar gene and protein structures. Transcriptomal and qRT-PCR results showed that five genes were up-regulated and five genes were down-regulated under salt stress. Among them, GmGRAS14, GmGRAS33 and GmGRAS69 had the highest fold of up-regulation at 12 h of salt treatment. GmGRAS17, GmGRAS54 and GmGRAS57 showed the highest down-regulation multiple at 12 h of salt treatment, suggesting that these genes may play an important role in the response to salt stress of soybean.

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备注/Memo

备注/Memo:
收稿日期：2020-05-11基金项目：湖南科技学院重点项目（17XKY012）；湖南省大学生研究性学习和创新性实验计划项目[湘教通(2018)255号]作者简介：张斌(1981-)，男，湖南永州人，博士，讲师，主要从事植物发育生物学研究。(E-mail)zhangbin27104@163.com通讯作者：唐满生，(E-mail)manshengtang@sina.com
更新日期/Last Update: 2021-05-10