[1]陈艳,邓昌蓉,侯全刚,等.UV-B辐射对不同品种(品系)辣椒幼苗光合特性及UVR8表达的影响[J].江苏农业学报,2023,(07):1449-1459.[doi:doi:10.3969/j.issn.1000-4440.2023.07.002]
　CHEN Yan,DENG Chang-rong,HOU Quan-gang,et al.Effects of UV-B radiation on photosynthetic characteristics and UVR8 expression in different pepper seedlings[J].,2023,(07):1449-1459.[doi:doi:10.3969/j.issn.1000-4440.2023.07.002]
点击复制

UV-B辐射对不同品种(品系)辣椒幼苗光合特性及UVR8表达的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年07期
页码:
1449-1459
栏目:
遗传育种·生理生化
出版日期:
2023-10-31

文章信息/Info

Title:
Effects of UV-B radiation on photosynthetic characteristics and UVR8 expression in different pepper seedlings
作者:
陈艳1; 2; 邓昌蓉1; 2; 3; 侯全刚1; 2; 3; 文军琴1; 2; 3; 刘敏1; 2; 邵登魁1; 2; 3
(1.青海大学农林科学院,青海西宁810016；2.青海省蔬菜遗传与生理重点实验室,青海西宁810016；3.青藏高原种质资源研究与利用实验室,青海西宁810016)
Author(s):
CHEN Yan1; 2; DENG Chang-rong1; 2; 3; HOU Quan-gang1; 2; 3; WEN Jun-qin1; 2; 3; LIU Min1; 2; SHAO Deng-kui1; 2; 3
(1.Qinghai Academy of Agriculture and Forestry Sciences, Xining 810016, China;2.Qinghai Key Laboratory of Vegetable Genetics and Physiology, Xining 810016, China;3.Laboratory for Research and Utilization of Germplasm Resources in Qinghai Tibet Plateau, Xining 810016, China)
关键词:
辣椒; UV-B辐射; 光合特性; UVR8
Keywords:
chili; UV-B radiation; photosynthetic characteristics; UVR8
分类号:
S624.4+3
DOI:
doi:10.3969/j.issn.1000-4440.2023.07.002
文献标志码:
A
摘要:
为了解紫外线B(UV-B)辐射对辣椒(Capsicum annuum L.)光合特性及UV-B应答基因UVR8表达量的影响,以青海省主栽的9个辣椒品种(品系)为试验材料,紫外线处理剂量为28.56 kJ/(m2·d),设空白对照(无照射),测定幼苗叶片光合特性、形态指标等14个指标,同时利用实时荧光定量PCR技术检测UVR8基因在各试验材料中的相对表达量。结果表明,与对照相比，处理组辣椒的叶绿素、类胡萝卜素含量总体降低,光合指标总体下降,从而抑制叶片光合作用,总体上UV-B处理对华美105大多数光合指标的影响较大,对乐都长辣椒的影响较小。荧光定量PCR检测结果显示,UV-B处理后，UVR8在华美105中的相对表达量上升幅度最大且与对照间的差异达显著水平。综合分析可知,华美105的大多数指标显著受到抑制,光能利用率降低,因此华美105为UV-B敏感型品种,且UVR8在该品种中的表达水平较高,说明UVR8能够在不耐紫外辐射辣椒品种(品系)中积极响应UV-B胁迫。
Abstract:
In order to understand the effects of ultraviolet B (UV-B) radiation on photosynthetic characteristics and UV-B response gene UVR8 expression of capsicum (Capsicum annuum L.), nine varieties (lines) of capsicum in Qinghai province were selected as test materials, the treatment dose was 28.56 kJ/(m2·d), and blank control was set. A total of 14 indices such as photosynthetic characteristics and morphological indices of seedling leaves were determined. Meanwhile, the expression level of UVR8 was detected by real-time fluorescence quantitative PCR. The results showed that compared with the control, the chlorophyll and carotenoid contents in the treatment group were generally reduced, and the photosynthetic characteristics generally decreased, which inhibited the photosynthesis of leaves. Overall, UV-B treatment had a greater effect on most photosynthetic indices of Huamei 105, and a smaller effect on Ledu long pepper. The results of fluorescence quantitative PCR showed that the relative expression of UVR8 in Huamei 105 increased the most after UV-B treatment and was significantly higher than that in the control group. In conclusion, most indices of Huamai 105 were significantly inhibited, and the utilization rate of light energy was decreased. Therefore, Huamei 105 was a UV-B sensitive variety, and UVR8 was expressed at a high level in this variety, indicating that UVR8 could actively respond to UV-B stress in the varieties that were not resistant to ultraviolet radiation.

参考文献/References:

[1]付秋实,李红岭,崔健,等. 水分胁迫对辣椒光合作用及相关生理特性的影响[J]. 中国农业科学,2009,42(5):1859-1866.
[2]彭书练. 辣椒功能成分的综合提取技术研究[D]. 长沙：湖南农业大学,2007.
[3]叶超. 青海循化线辣椒带富撒拉人家[N]. 西部时报,2008-11-25(13).
[4]钟楚,陈宗瑜,王毅,等. UV-B辐射对植物影响的分子水平研究进展[J]. 生态学杂志,2009,28(1):129-137.
[5]李元,王勋陵. 紫外辐射增加对春小麦生理、产量和品质的影响[J]. 环境科学学报,1998(5):58-63.
[6]杨景宏,陈拓,王勋陵. 增强紫外线B辐射对小麦叶绿体膜组分和膜流动性的影响[J]. 植物生态学报,2000(1):102-105.
[7]林文雄,吴杏春,梁义元,等. UV-B辐射胁迫对水稻叶绿素荧光动力学的影响[J]. 中国生态农业学报,2002,10(1):8-12.
[8]TEVINI M,TERAMURA A H. UV-B effects on terrestrial plants[J]. Photochemistry and Photobiology, 1989, 50(4): 479-487.
[9]杜照奎. UV-B辐射增强对花生的生理生态影响及其分子机制[D]. 重庆：西南大学,2014.
[10]ARNON D I . Copper enzymes in isolated chloroplasts[J]. Plant Physiology, 1949, 24(1): 1-15.
[11]边海燕,钟启文,黄思杰,等. 干旱胁迫下大蒜果聚糖代谢关键酶基因的表达分析[J]. 分子植物育种,2018,16(20):6770-6776．
[12]赵敬. 辣椒内参基因的筛选及NBS-LRR类抗病基因同源序列的鉴定[D]. 南京：南京农业大学,2012.
[13]张宇,周自云,夏鹏国,等．干旱胁迫对柴胡中皂苷合成关键酶基因表达及皂苷含量的影响[J]. 中国中药杂志,2016,41(4):643-647.
[14]LIVAK K J, SCHMITTGEN T. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ct method[J]. Methods,2001,25(4):402-408.
[15]陈慧泽,韩榕. 植物响应UV-B辐射的研究进展[J]. 植物学报,2015,50(6):790-801.
[16]CALDWELL M M, BJRN L O, BORNMAN J F, et al. Effects of increased solar ultraviolet radiation on terrestrial ecosystems[J]. Journal of Photochemistry and Photobiology B: Biology, 1998, 46(1/2/3): 40-52.
[17]李俊,牛金文,杨芳,等. 不同马铃薯品种(系)对增强UV-B辐射的形态响应[J]. 中国生态农业学报,2016,24(6):770-779.
[18]JANSEN M A K, GABA V, GREENBERG B M. Higher plants and UV-B radiation: balancing damage, repair and acclimation[J]. Trends in Plant Science,1998,3(4):131-135.
[19]BARNES P W, FLINT S D, CALDWELL M M. Morphological responses of crop and weed species of different growth forms to ultraviolet-B radiation[J]. American Journal of Botany, 1990,77(10):1354-1360.
[20]LI Y, ZU Y Q, CHEN J J. et al. Intraspecific responses in crop growth and yield of 20 soybean cultivars to enhanced ultraviolet-B radiation under field conditions[J]. Field Crops Research,2002,78(1):1-8.
[21]王传海,郑有飞,何都良,等. 南京地区近地面紫外辐射UV-B强度对小麦生长及产量影响的评估[J]. 农业环境科学学报,2003(2):147-149.
[22]蔡锡安,夏汉平,彭少麟. 增强UV-B辐射对植物的影响[J]. 生态环境,2007(3):1044-1052.
[23]SWARNA K, BHANUMATHI G, MURTHY S. Studies on the UV-B radiation induced oxidative damage in thylakoid photofunctions and analysis of the role of antioxidant enzymes in maize primary leaves[J]. Bioscan,2012,7: 609-610.
[24]CHEN H Z, HAN R. He-Ne laser treatment improves the photosynthetic efficiency of wheat exposed to enhanced UV-B radiation[J]. Laser Physics,2014,24(10): 105602.
[25]耿东梅,单立山,李毅,等. 土壤水分胁迫对红砂幼苗叶绿素荧光和抗氧化酶活性的影响[J]. 植物学报,2014,49(3):282-291.
[26]韩雯,韩榕. 不同时间的 UV-B 辐射对拟南芥幼苗生长的影响[J].植物学报,2015,50(1): 40-46.
[27]张玉红,陈路瑶,刘彤,等.增补 UV-B 辐射对药用植物黄檗幼苗生长及光合生理影响[J]. 中国农学通报,2018,34(4)：76-82.
[28]李雪莹,殷红,战莘晔,等.添加 UV-B 辐射对灌浆期水稻剑叶光合特性及抗氧化能力的影响[J]. 沈阳农业大学学报,2017,48(3): 271-276.
[29]祁虹,段留生,王树林,等. 全生育期UV-B辐射增强对棉花生长及光合作用的影响[J]. 中国生态农业学报,2017,25(5): 708-719
[30]刘明雪,孙梅,王宇,等. 植物 UV-B 受体及其介导的光信号转导[J]. 植物学报,2012,47(6): 661-669.
[31]张君豪. 茄子UVR8基因的克隆与表达分析[D].上海：上海交通大学,2019.
[32]李晓一,詹亚光,娄晓瑞,等. 白桦BpUVR8基因的序列与表达模式分析[J]. 植物生理学报,2016,52(5): 685-692.

相似文献/References:

[1]吕敏,苏建坤,白和盛,等.桃蚜取食和机械损伤对番茄和辣椒 PAL、LOX 和 PPO 活性的诱导作用[J].江苏农业学报,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
　Lv?Min,SU Jian-kun,BAI He-sheng,et al.The activities of PAL, LOX and PPO in tomato and pepper plants induced by aphid herbivory and mechanical damage[J].,2016,(07):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
[2]吴淑华,赵文浩,李廷芳,等.南京辣椒上一种斑驳类型病毒病的分子鉴定[J].江苏农业学报,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
　WU Shu-hua,ZHAO Wen-hao,LI Ting-fang,et al.Molecular identification of a virus causing mottle symptoms in pepper leaves in Nanjing[J].,2015,(07):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
[3]郭广君,孙茜,刘金兵,等.基于辣椒基因组重测序的InDel标记开发及应用[J].江苏农业学报,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
　GUO Guang-jun,SUN Qian,LIU Jin-bing,et al.Development and application of pepper InDel markers based on genome re-sequencing[J].,2015,(07):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
[4]李廷芳,吴淑华,赵文浩,等.青海海东设施辣椒轻斑驳病毒的分子检测[J].江苏农业学报,2017,(04):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
　LI Ting-fang,WU Shu-hua,ZHAO Wen-hao,et al.Molecular detection of mild mottle virus isolated from pepper in Haidong, Qinghai province[J].,2017,(07):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
[5]刘潮,韩利红,宋培兵,等.辣椒类甜蛋白基因家族鉴定及表达分析[J].江苏农业学报,2018,(01):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
　LIU Chao,HAN Li-hong,SONG Pei-bing,et al.Identification and expression analysis of thaumatin-like protein gene in pepper[J].,2018,(07):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
[6]王运儒,秦玉燕,杨秀娟,等.40％氯虫·噻虫嗪水分散粒剂在辣椒及土壤中的残留消解动态[J].江苏农业学报,2018,(01):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
　WANG Yun-ru,QIN Yu-yan,YANG Xiu-juan,et al.Dissipation of chlorantraniliprole and thiamethoxam in pepper and soil after field application in the form of 40% water dispersible granules[J].,2018,(07):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
[7]潘宝贵,钱恒彦,戈伟,等.辣椒应答冷信号转导机制研究进展[J].江苏农业学报,2019,(03):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
　PAN Bao-gui,QIAN Heng-yan,GE Wei,et al.Research progress of cold signal transduction mechanisms in pepper[J].,2019,(07):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
[8]高晶霞,吴雪梅,牛勇琴,等.辣根素水乳剂对连作辣椒生长及土壤酶活性的影响[J].江苏农业学报,2021,(01):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
　GAO Jing-xia,WU Xue-mei,NIU Yong-qin,et al.Effect of athomin water emulsion on growth and soil enzyme activities of continuous cropping pepper[J].,2021,(07):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
[9]郭广君,朱雪梅,潘宝贵,等.利用InDel分子标记辅助选育辣椒抗黄瓜花叶病毒病种质[J].江苏农业学报,2021,(05):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
　GUO Guang-jun,ZHU Xue-mei,PAN Bao-gui,et al.Innovation of pepper germplasm resource with resistance to cucumber mosaic virus by InDel molecular marker assisted selection[J].,2021,(07):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
[10]刘雪梅,王悦,李玮琦,等.辣椒miR169及其靶基因的生物信息学分析[J].江苏农业学报,2021,(06):1510.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
　LIU Xue-mei,WANG Yue,LI Wei-qi,et al.Bioinformatics analysis of miR169 and its target genes in pepper[J].,2021,(07):1510.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]

备注/Memo

备注/Memo:
收稿日期：2022-10-30基金项目：青海省科技厅应用基础研究项目(2020-ZJ-766) 作者简介：陈艳(1996-)，女，甘肃定西人，硕士研究生，研究方向为辣椒耐紫外生理及遗传特性研究。(E-mail)cychelsea960228@163.com通讯作者：邵登魁，(Tel)0971-5311240；(E-mail)sdk2003-2003-2003@163.com
更新日期/Last Update: 2023-11-17