[1]刘潮,韩利红,宋培兵,等.辣椒类甜蛋白基因家族鉴定及表达分析[J].江苏农业学报,2018,(01):122-129.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
　LIU Chao,HAN Li-hong,SONG Pei-bing,et al.Identification and expression analysis of thaumatin-like protein gene in pepper[J].,2018,(01):122-129.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
点击复制

辣椒类甜蛋白基因家族鉴定及表达分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
122-129
栏目:
园艺
出版日期:
2018-02-25

文章信息/Info

Title:
Identification and expression analysis of thaumatin-like protein gene in pepper
作者:
刘潮; 韩利红; 宋培兵; 王德琴; 王海波; 唐利洲
(曲靖师范学院云南高原生物资源保护与利用研究中心/生物资源与食品工程学院/云南省高校云贵高原动植物多样性及生态适应性进化重点实验室，云南 曲靖 655011)
Author(s):
LIU Chao; HAN Li-hong; SONG Pei-bing; WANG De-qin; WANG Hai-bo; TANG Li-zhou
(Center for Yunnan Plateau Biological Resources Protection and Utilization/College of Biological Resource and Food Engineering/Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yung
关键词:
辣椒; 类甜蛋白基因; 生物信息学
Keywords:
Capsicum annuum; thaumatin-like protein gene; bioinformatics
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2018.01.018
文献标志码:
A
摘要:
为了在全基因组水平上了解辣椒类甜蛋白基因（TLP）家族特征，本研究通过生物信息学方法，利用辣椒基因组数据库，对筛选的28个辣椒TLP家族成员进行鉴定，对结构功能、聚类及时空表达进行分析。结果表明，辣椒TLP家族基因主要分为4种结构类型，基因分布在9条染色体上。系统发育分析结果显示，辣椒TLP家族基因属于8个聚类组，其中成员最多的聚类组5中的基因主要来自1号染色体。基因启动子区顺式作用元件分析发现多个激素响应元件、生物/非生物响应元件。时空表达分析结果表明，多数辣椒TLP家族基因在各器官和果实发育的各阶段均有较高表达，各成员在时空表达上具有器官特异性。本研究为辣椒TLP家族基因的克隆及其在植物生长及对抗胁迫方面的研究奠定了基础。
Abstract:
To understand the characteristics of pepper thaumatin-like protein (TLP) gene family on the genomic level, gene structure and phylogenesis of 28 pepper TLP family members were analyzed and identified through pepper genome database and bioinformatics methods. The results showed that the pepper TLP gene structure was classified into four types, and TLP genes distributed on nine of twelve chromosomes. Phylogenetic analysis showed that the pepper TLP family genes were classified into eight evolutionary groups. Most members of group five mainly derived from the first chromosome. The spatiotemporal expression analysis showed that most of the TLP family genes were highly expressed in various organs and fruit development stages. Each member had organ specificity in temporal and spatial expression. This study laid a foundation for cloning of pepper TLP genes and further functional dissection in organ development.

参考文献/References:

[1]ABAD L R， D′URZO M P，LIU D，et al．Antifungal activity of tobacco osmotin has specificity and involves plasma membrane permeabilization[J]．Plant Science，1996，118（1）：11-23.
[2]SHATTERS R G，BOYKIN L M，LAPOINTE S L，et al．Phylogenetic and structural relationships of the PR5 gene family reveal an ancient multigene family conserved in plants and select animal taxa[J]．Journal of Molecular Evolution，2006，63（1）：12-29.
[3]LI X Y，GAO L，ZHANG W H，et al．Characteristic expression of wheat PR5 gene in response to infection by the leaf rust pathogen，Puccinia triticina[J]．Journal of Plant Interactions，2015，10（1）：132-141.
[4]ROUT E，NANDA S，JOSHI R K．Molecular characterization and heterologous expression of a pathogen induced PR5 gene from garlic （Allium sativum L.） conferring enhanced resistance to necrotrophic fungi[J]．European Journal of Plant Pathology，2016，144（2）：345-360.
[5]刘潮,韩利红,宋培兵,等. 桑树类甜蛋白家族鉴定与生物信息学分析[J].江苏农业学报,2017,33(5):998-1006.
[6]HE R，WU J，ZHANG Y，et al．Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine[J]．Protoplasma，2017，254（4）：1579-1589.
[7]XUE X，CAO Z X，ZHANG X T，et al．Overexpression of OsOSM1 enhances resistance to rice sheath blight[J]．Plant Disease，2016，100（8）：1634-1642.
[8]MISRA R C，KAMTHAN M，KUMAR S，et al．A thaumatin-like protein of Ocimum basilicum confers tolerance to fungal pathogen and abiotic stress in transgenic Arabidopsis[J]．Scientific Reports，2016，6：25340.
[9]SINGH N K，KUMAR K R R，KUMAR D，et al．Characterization of a pathogen induced thaumatin-like protein gene AdTLP from Arachis diogoi，a wild peanut[J]．PLoS One，2013，8（12）：e83963.
[10]GUO J，ZHAO X，WANG H，et al．Expression of the LePR5 gene from cherry tomato fruit induced by Cryptococcus laurentii and the analysis of LePR5 protein antifungal activity[J]．Postharvest Biology and Technology，2016，111：337-344.
[11]FILS-LYCAON B R，WIERSMA P A，Eastwell K C，et al．Cherry protein and its gene，abundantly expressed in ripening fruit，have been identified as thaumatin-like[J]．Plant Physiology，1996，111（1）：269-273.
[12]KIM S H，LEE J R，KIM S R．Molecular characterization of a fruit-preferential thaumatin-like gene from apple （Malus domestica cv．Fuji）[J]．Journal of Plant Biology，2003，46（1）：52-58.
[13]HO V S，WONG J H，NG T B．A thaumatin-like antifungal protein from the emperor banana[J]．Peptides，2007，28（4）：760-766.
[14]LA MARCELINO J，SILVA P，SANTOS M C，et al．Food anaphylaxis associated with thaumatin-like proteins[J]．Allergy：European Journal of Allergy and Clinical Immunology，2015，70：473.
[15]LIU J J，STURROCK R，EKRAMODDOULLAH A K M．The superfamily of thaumatin-like proteins：its origin，evolution，and expression towards biological function[J]．Plant Cell Reports，2010，29（5）：419-436.
[16]ZHAO J P，SU X H．Patterns of molecular evolution and predicted function in thaumatin-like proteins of Populus trichocarpa[J]．Planta，2010，232（4）：949-962.
[17]CAO J，LV Y，HOU Z，et al．Expansion and evolution of thaumatin-like protein （TLP） gene family in six plants[J]．Plant Growth Regulation，2016，79（3）：299-307.
[18]PETRE B，MAJOR I，ROUHIER N，et al．Genome-wide analysis of eukaryote thaumatin-like proteins （TLPs） with an emphasis on poplar[J]．Plant Biology，2011，11（1）：33.
[19]IVEY M L L，STRAYER A，SIDHU J K，et al．Bacterial leaf spot of bell pepper （Capsicum annuum） in louisiana is caused by Xanthomonas euvesicatoria pepper races 1 and 3[J]．Plant Disease，2016，100（4）：853.
[20]XIA H，WANG X L，ZHU H J，et al．First report of anthracnose caused by Glomerella acutata on chili pepper in China[J]．Plant Disease，2011，95（2）：219.
[21]KIM S，PARK M，YEOM S I，et al．Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species[J]．Nature Genetics，2014，46（3）：270-278.
[22]QIN C，YU C，SHEN Y，et al．Whole-genome sequencing of cultivated and wild peppers provides insights into Capsicum domestication and specialization[J]．Proceedings of the National Academy of Sciences of the United States of America，2014，111（14）：5135-5140.
[23]LIU D，HE X，LI W，et al．Molecular cloning of a thaumatin-like protein gene from Pyrus pyrifolia and overexpression of this gene in tobacco increased resistance to pathogenic fungi[J]．Plant Cell，Tissue and Organ Culture，2012，111（1）：29-39.
[24]NARASIMHAN M L，BRESSAN R A，D′URZO M P，et al．Unexpected turns and twists in structure/function of PR-proteins that connect energy metabolism and immunity[J]．Advances in Botanical Research，2009，51（9）：439-489.
[25]WANG L，YANG L，ZHANG J，et al．Cloning and characterization of a thaumatin-like protein gene PeTLP in Populus deltoides× P．euramericana cv.‘Nanlin895′[J]．Acta Physiologiae Plantarum，2013，35（10）：2985-2998.
[26]HAYASHI M，SHIRO S，KANAMORI H，et al．A thaumatin-like protein，Rj4，controls nodule symbiotic specificity in soybean[J]．Plant and Cell Physiology，2014，55（9）：1679-1689.
[27]MITSUHARA I，IWAI T，SEO S，et al．Characteristic expression of twelve rice PR1 family genes in response to pathogen infection，wounding，and defense-related signal compounds[J]．Molecular Genetics and Genomics，2008，279（4）：415-427.

相似文献/References:

[1]吕敏,苏建坤,白和盛,等.桃蚜取食和机械损伤对番茄和辣椒 PAL、LOX 和 PPO 活性的诱导作用[J].江苏农业学报,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
　Lv?Min,SU Jian-kun,BAI He-sheng,et al.The activities of PAL, LOX and PPO in tomato and pepper plants induced by aphid herbivory and mechanical damage[J].,2016,(01):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
[2]吴淑华,赵文浩,李廷芳,等.南京辣椒上一种斑驳类型病毒病的分子鉴定[J].江苏农业学报,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
　WU Shu-hua,ZHAO Wen-hao,LI Ting-fang,et al.Molecular identification of a virus causing mottle symptoms in pepper leaves in Nanjing[J].,2015,(01):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
[3]郭广君,孙茜,刘金兵,等.基于辣椒基因组重测序的InDel标记开发及应用[J].江苏农业学报,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
　GUO Guang-jun,SUN Qian,LIU Jin-bing,et al.Development and application of pepper InDel markers based on genome re-sequencing[J].,2015,(01):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
[4]李廷芳,吴淑华,赵文浩,等.青海海东设施辣椒轻斑驳病毒的分子检测[J].江苏农业学报,2017,(04):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
　LI Ting-fang,WU Shu-hua,ZHAO Wen-hao,et al.Molecular detection of mild mottle virus isolated from pepper in Haidong, Qinghai province[J].,2017,(01):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
[5]王运儒,秦玉燕,杨秀娟,等.40％氯虫·噻虫嗪水分散粒剂在辣椒及土壤中的残留消解动态[J].江苏农业学报,2018,(01):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
　WANG Yun-ru,QIN Yu-yan,YANG Xiu-juan,et al.Dissipation of chlorantraniliprole and thiamethoxam in pepper and soil after field application in the form of 40% water dispersible granules[J].,2018,(01):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
[6]潘宝贵,钱恒彦,戈伟,等.辣椒应答冷信号转导机制研究进展[J].江苏农业学报,2019,(03):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
　PAN Bao-gui,QIAN Heng-yan,GE Wei,et al.Research progress of cold signal transduction mechanisms in pepper[J].,2019,(01):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
[7]高晶霞,吴雪梅,牛勇琴,等.辣根素水乳剂对连作辣椒生长及土壤酶活性的影响[J].江苏农业学报,2021,(01):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
　GAO Jing-xia,WU Xue-mei,NIU Yong-qin,et al.Effect of athomin water emulsion on growth and soil enzyme activities of continuous cropping pepper[J].,2021,(01):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
[8]郭广君,朱雪梅,潘宝贵,等.利用InDel分子标记辅助选育辣椒抗黄瓜花叶病毒病种质[J].江苏农业学报,2021,(05):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
　GUO Guang-jun,ZHU Xue-mei,PAN Bao-gui,et al.Innovation of pepper germplasm resource with resistance to cucumber mosaic virus by InDel molecular marker assisted selection[J].,2021,(01):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
[9]刘雪梅,王悦,李玮琦,等.辣椒miR169及其靶基因的生物信息学分析[J].江苏农业学报,2021,(06):1510.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
　LIU Xue-mei,WANG Yue,LI Wei-qi,et al.Bioinformatics analysis of miR169 and its target genes in pepper[J].,2021,(01):1510.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
[10]朱雪梅,郭广君,潘宝贵,等.辣椒抗PMMoV基因L4连锁标记的验证分析[J].江苏农业学报,2022,38(06):1627.[doi:doi:10.3969/j.issn.1000-4440.2022.06.022]
　ZHU Xue-mei,GUO Guang-jun,PAN Bao-gui,et al.Test analysis of L4-linked markers for PMMoV resistant gene in pepper[J].,2022,38(01):1627.[doi:doi:10.3969/j.issn.1000-4440.2022.06.022]

备注/Memo

备注/Memo:
收稿日期：2017-07-18 基金项目：国家自然科学基金项目（31460179）；云南省高校科技创新团队项目[云教科(2014)14号] 作者简介：刘潮（1980-），男，河北景县人，博士，讲师，研究方向为分子植物病理学。（E-mail）liuchao@mail.qjnu.edu.cn 通讯作者：唐利洲，（E-mail）tanglizhou@163.com
更新日期/Last Update: 2018-03-06