[1]刘雪梅,王悦,李玮琦,等.辣椒miR169及其靶基因的生物信息学分析[J].江苏农业学报,2021,(06):1510-1515.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
 LIU Xue-mei,WANG Yue,LI Wei-qi,et al.Bioinformatics analysis of miR169 and its target genes in pepper[J].,2021,(06):1510-1515.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
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辣椒miR169及其靶基因的生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年06期
页码:
1510-1515
栏目:
园艺
出版日期:
2021-12-30

文章信息/Info

Title:
Bioinformatics analysis of miR169 and its target genes in pepper
作者:
刘雪梅王悦李玮琦魏继承任如意郝爱平
(牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011)
Author(s):
LIU Xue-meiWANG YueLI Wei-qiWEI Ji-chengREN Ru-yiHAO Ai-ping
( College of Life Sciences and Technology, Mudanjiang Normal College, Mudanjiang 157011, China)
关键词:
miR169辣椒生物信息学NF-YA
Keywords:
miR169pepperbioinformaticsNF-YA
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2021.05.020
文献标志码:
A
摘要:
为了解miR169基因家族在辣椒生长发育过程的重要调控作用,通过生物信息学的方法对can-miR169基因家族的成员进行了染色体定位、序列保守性分析、系统发育进化树分析、前体序列二级结构预测和靶基因预测。结果表明:can-miR169家族成员定位在4条染色体上;成熟序列高度保守,前体序列在产生成熟序列的位置高度保守;预测结果显示can-miR169家族成员均有靶基因,这些靶基因主要以靶基因核因子A(NF-YA)复合物为主,且大体相同。这些靶基因可以参与到种子萌发、植株发育和对外界环境胁迫的应答等多种生命活动中。
Abstract:
In order to understand the important regulatory role of miR169 gene family in the growth and development of pepper, the members of can-miR169 gene family were analyzed by bioinformatics, including chromosome mapping, sequence conservation analysis, phylogenetic tree analysis, precursor sequence secondary structure prediction and target gene prediction. The results showed that the members of can-miR169 family were located on four chromosomes, the mature sequence was highly conserved. The precursor sequence was highly conserved in the position where the mature sequence was produced. The forecasting results indicated that all members of can-miR169 family had target genes, which were mainly composed of nuclear factor Y (NF-YA) complexes and were roughly the same. These target genes can be involved in a variety of life activities such as seed germination, plant development and response to external environmental stress.

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备注/Memo

备注/Memo:
收稿日期:2021-04-20基金项目:牡丹江师范学院科研项目(YB2020005);黑龙江省省属高等学校基本科研业务费科研项目(1353MSYYB011)作者简介:刘雪梅(1996-)女,贵州黔东南人,硕士,研究方向为分子生物学与生物化学。(E-mail)lxm12252021@163.com通讯作者:郝爱平,(E-mail)swxhap@126.com
更新日期/Last Update: 2022-01-07