[1]赵嫚,陈仕勇,李亚萍,等.外源GABA对盐胁迫下金花菜种子萌发及幼苗抗氧化能力的影响[J].江苏农业学报,2021,(02):310-316.[doi:doi:10.3969/j.issn.1000-4440.2021.02.005]
　ZHAO Man,CHEN Shi-yong,LI Ya-ping,et al.Influence of exogenous γ-aminobutyric acid (GABA) on seed germination and antioxidant protection of Medicago polymorpha under salt stress[J].,2021,(02):310-316.[doi:doi:10.3969/j.issn.1000-4440.2021.02.005]
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外源GABA对盐胁迫下金花菜种子萌发及幼苗抗氧化能力的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年02期
页码:
310-316
栏目:
遗传育种·生理生化
出版日期:
2021-04-30

文章信息/Info

Title:
Influence of exogenous γ-aminobutyric acid (GABA) on seed germination and antioxidant protection of Medicago polymorpha under salt stress
作者:
赵嫚1; 2; 陈仕勇1; 2; 李亚萍2; 周青平2; 陈有军2; 常馨丹1
(1.西南民族大学畜牧兽医学院，四川成都610041；2.四川省抗逆牧草种质资源创新及生态修复工程实验室，四川成都610041）
Author(s):
ZHAO Man1; 2; CHEN Shi-yong1; 2; LI Ya-ping2; ZHOU Qing-ping2; CHEN You-jun2; CHANG Xin-dan1
（1.College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China;2.Sichuan Provincial Engineering Laboratory of Stress Tolerance Forage Germplasm Innovation and Ecological Restoration, Chengdu 610041, China）
关键词:
金花菜; 盐胁迫; 种子萌发; GABA; 抗氧化保护
Keywords:
Medicago polymorpha L.; salt stress; seed germination; γ-aminobutyric acid (GABA); antioxidant protection
分类号:
S54
DOI:
doi:10.3969/j.issn.1000-4440.2021.02.005
文献标志码:
A
摘要:
以楚雄金花菜种子为研究材料，通过测定种子萌发特性、胚芽鲜质量、胚芽干质量、胚根鲜质量、胚根干质量、胚根长、超氧阴离子（O2·-）含量、过氧化氢（H2O2）含量、丙二醛（MDA）含量以及抗氧化酶活性，研究外源GABA（γ-氨基丁酸）对盐胁迫下金花菜种子萌发及幼苗生长的影响。结果表明，0.5～2.0 μmol/L GABA预处理提高了盐胁迫下金花菜种子的发芽势、活力指数，并显著缩短了平均萌发时间。100 mmol/L盐胁迫对幼苗胚芽鲜质量无影响，但显著降低了胚芽干质量、胚根干质量、胚根鲜质量及胚根长，而0.5～2.0 μmol/L GABA预处理诱导了胚芽和胚根鲜质量、干质量及胚根长的增加。GABA预处理不同程度地提高了盐胁迫下金花菜幼苗超氧化物歧化酶（SOD)、过氧化物酶 (POD)、过氧化氢酶 (CAT)和抗坏血酸过氧化物酶 (APX)活性，降低了O2·-、H2O2以及MDA含量。GABA能够激活抗氧化酶活性，降低盐胁迫诱导的氧化伤害和膜损伤，改善了金花菜在种子萌发阶段的抗盐性，且在0.5～2.0 μmol/L浓度范围内均有效果。
Abstract:
Using the seed of Medicago polymorpha cv. Chuxiong as material, the effect of exogenous γ-aminobutyric acid (GABA) on seed germination and seedling growth under salt stress were determined by analyzing seed germination characteristics, fresh and dry weight of embryos and radicles, radicle length, superoxide anion (O2·-) content, hydrogen peroxide (H2O2) content, malonaldehyde (MDA) content and antioxidant enzyme activities. The results showed that the seed germination potential and vigor indexes of M. polymorpha cv. Chuxiong under salt stress were enhanced after pretreated with 0.5-2.0 μmol/L GABA, and the mean germination time was shortened. Salt stress with 100 mmol/L NaCl showed no effect on the fresh weight of embryos, but the dry weight of embryos and radicles, the fresh weight of radicles and radicle length were significantly reduced, while the pretreatment with 0.5-2.0 μmol/LGABA increased the fresh and dry weight of radicles and radical length. Pretreatment of GABA for M. polymorpha cv. Chuxiong seedlings under salt stress improved the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), decreased the contents of O2·-, H2O2 and MDA in varying degrees. The results suggest that GABA can activate the activities of antioxidant enzymes, reduce the oxidation damage and membrane injury induced by salt stress, leading to the improved salt tolerance of M. polymorpha cv. Chuxiong in seed germination stage, and the effective concentration scope of GABA is 0.5-2.0 μmol/L.

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备注/Memo

备注/Memo:
收稿日期：2020-08-23基金项目：国家重点研发计划项目（2017YFC0504806）；西南民族大学中央高校基本科研业务费专项（2020PTJS25）作者简介：赵嫚（1995-），女，贵州毕节人，硕士研究生，主要从事牧草种质资源评价研究。（E-mail）1301036256@qq.com通讯作者：陈仕勇，（E-mail）chengshi8827@163.com
更新日期/Last Update: 2021-05-10