[1]韩金龙,李慧,蔺经,等.核黄素对盐胁迫下杜梨叶片抗氧化系统的影响[J].江苏农业学报,2015,(04):893-898.[doi:10.3969/j.issn.1000-4440.2015.04.029]
　HAN Jing-long,LI Hui,LIN Jing,et al.The regulatory role of riboflavin in antioxidant system of Pyrus betulaefolia in response to salt tolerance[J].,2015,(04):893-898.[doi:10.3969/j.issn.1000-4440.2015.04.029]
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核黄素对盐胁迫下杜梨叶片抗氧化系统的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
893-898
栏目:
园艺
出版日期:
2015-08-31

文章信息/Info

Title:
The regulatory role of riboflavin in antioxidant system of Pyrus betulaefolia in response to salt tolerance
作者:
韩金龙12; 李慧1; 蔺经1; 杨青松1; 常有宏1
（1.江苏省农业科学院园艺研究所/江苏省高效园艺作物遗传改良重点实验室，江苏南京210014；2.南京农业大学园艺学院，江苏南京210095）
Author(s):
HAN Jing-long12; LI Hui1; LIN Jing1; YANG Qing-song1; CHANG You-hong1
（1.Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China;2.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China）
关键词:
杜梨; 核黄素; 盐胁迫; 过氧化作用; 抗氧化酶
Keywords:
Pyrus betulaefolia; riboflavin; salt stress; peroxidation; antioxidant enzyme
分类号:
S661.2
DOI:
10.3969/j.issn.1000-4440.2015.04.029
文献标志码:
A
摘要:
为探讨外源核黄素对盐胁迫下杜梨叶片抗氧化系统的影响，以梨砧木杜梨幼苗为供试材料，在水培条件下，研究了外源施加不同浓度核黄素对200 mmol/L NaCl胁迫下其叶片抗氧化酶活性、活性氧产生、膜质过氧化和抗氧化物质含量的影响。结果显示：NaCl胁迫3 d后，杜梨叶片中超氧化物歧化酶（SOD）活性减弱，过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）、谷胱甘肽过氧化物酶（GSH-Px）、抗坏血酸过氧化物酶（APX）的活性增强，抗氧化物质谷胱甘肽（GSH）和抗坏血酸（AsA）合成下降，活性氧（O2·-、H2O2）和丙二醛（MDA）大量积累。施加外源核黄素能增强NaCl胁迫下杜梨叶片中SOD、POD、CAT、GR、GSH-Px和APX的活性，提高GSH和AsA的含量，减少O2·-和H2O2的产生，降低脂质过氧化程度，有效缓解盐胁迫对杜梨叶片的过氧化伤害，其中以10 μmol/L浓度的核黄素处理效果最为显著。
Abstract:
To explore the effect of exogenous riboflavin on the antioxidant system in the leaves of Pyrus betulaefolia under salt stress, the antioxidant enzymes activities, the production of active oxygen, lipid peroxidation and antioxidants contents of the leaves of P. betulaefolia were analysed when its seedlings under 200 mmol/L NaCl stress were exposed to different concentrations (0 μmol/L, 5 μmol/L, 10 μmol/L, 50 μmol/L, 100 μmol/L) of riboflavin in hydroponic culture, after 3-d salt stress, the activity of superoxide dismutase (SOD) was decreased, the activities of peroxidase (POD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GSH-Px) and ascorbic acid peroxidase (APX) were enhanced, the contents of GSH and AsA were reduced, and the accumulation of O2〖KG-1〗·〖KG-*8〗-, H2O2 and MDA were increased. Riboflavin application alleviated the damage caused by NaCl by improving the activities of SOD, POD, CAT, GR, GSH-Px and APX, increasing the contents of GSH and AsA, reducing the productions of O2〖KG-1〗·〖KG-*8〗- and H2O2 were decreased, and decreasing the degree of lipid peroxidation. The riboflavin with the concentration of 10 μmol/L showed the best effects.

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备注/Memo

备注/Memo:
收稿日期：2015-01-19 基金项目：江苏省农业科技自主创新基金项目[CX(14)5018]；国家自然科学基金项目（31372051） 作者简介：韩金龙（1989-），男，山西霍州人，硕士研究生，主要从事果树逆境生理和分子生物学研究。（E-mail）hjlong24@126.com 通讯作者：常有宏，（E-mail）cyh@jaas.ac.cn
更新日期/Last Update: 2015-08-31