[1]李敏,郭聪,李玉娟,等.旱柳转录组测序及生物学分析[J].江苏农业学报,2019,(02):271-281.[doi:doi:10.3969/j.issn.1000-4440.2019.02.005]
　LI Min,GUO Cong,LI Yu-juan,et al.Transcriptome sequencing and biological analysis of willow (Salix matsudana)[J].,2019,(02):271-281.[doi:doi:10.3969/j.issn.1000-4440.2019.02.005]
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旱柳转录组测序及生物学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年02期
页码:
271-281
栏目:
遗传育种·生理生化
出版日期:
2019-04-30

文章信息/Info

Title:
Transcriptome sequencing and biological analysis of willow (Salix matsudana)
作者:
李敏; 郭聪; 李玉娟; 冯新民; 王莹; 张健; 谈峰
（江苏沿江地区农业科学研究所，江苏南通226541）
Author(s):
LI Min; GUO Cong; LI Yu-juan; FENG Xin-min; WANG Ying; ZHANG Jian; TAN Feng
(Institute of Agricultural Sciences of Jiangsu Changjiang River Bank District, Nantong 226541, China)
关键词:
旱柳; 转录组分析; 盐胁迫; 差异表达基因
Keywords:
Salix matsudana; transcriptome analysis; salt stress; differentially expressed gene
分类号:
S718.43
DOI:
doi:10.3969/j.issn.1000-4440.2019.02.005
文献标志码:
A
摘要:
对盐敏感旱柳沿江柳和耐盐旱柳9901的杂交F1代根部进行了RNA-Seq测序和分析，共获取了107 950条Unigene，平均长度为1 076.96 bp。通过与COG、GO等8个数据库比对，60 848个基因获得注释信息，其中38 182条Unigene在GO数据库中获得注释，24 101条Unigene在KEGG数据库中获得注释。GO和KEGG富集分析结果表明，差异表达基因主要调节核糖体代谢、植物激素信号转导等生物学功能。
Abstract:
In this study, RNA-Seq sequencing and analysis were performed on the roots of F1 hybrids of salt sensitive Yanjiang Salix matsudana and salt tolerant 9901 S. matsudana, and a total of 107 950 unigenes were obtained. The average length was 1 076.96 bp. By comparing with eight databases including COG and GO, annotation informations of 60 848 genes were obtained, of which 38 182 unigenes were annotated in the GO database and 24 101 unigenes were annotated in the KEGG database. The GO and KEGG enrichment analysis results showed that differentially expressed genes regulated some biological functions, such as ribosome metabolism and phytohormone signal transduction.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2018-04-28 基金项目：江苏省重点研发计划“现代农业”项目(BE2016328)；第十四批“六大人才高峰”高层次人才项目（2017-NY-141） 作者简介：李敏（1981-），女，江苏如东人，硕士，副研究员，研究方向为耐盐林木育种与栽培，(E-mail)alice04@163.com 通讯作者：郭聪，(E-mail)guocong5532@126.com
更新日期/Last Update: 2019-05-05