[1]包颖,李泽卿,魏琳燕,等.月季盐胁迫响应转录因子基因RcMYB102的克隆及表达分析[J].江苏农业学报,2020,(06):1521-1528.[doi:doi:10.3969/j.issn.1000-4440.2020.06.023]
　BAO Ying,LI Ze-qing,WEI Lin-yan,et al.Cloning and expression analysis of the transcription factor gene RcMYB102 in response to salt stress in Rosa chinensis[J].,2020,(06):1521-1528.[doi:doi:10.3969/j.issn.1000-4440.2020.06.023]
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月季盐胁迫响应转录因子基因RcMYB102的克隆及表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1521-1528
栏目:
园艺
出版日期:
2020-12-31

文章信息/Info

Title:
Cloning and expression analysis of the transcription factor gene RcMYB102 in response to salt stress in Rosa chinensis
作者:
包颖1; 李泽卿2; 魏琳燕1; 陈超1; 付玲1; 张红心1
(1.唐山师范学院，河北唐山063000；2.中南林业科技大学风景园林学院，湖南长沙410000)
Author(s):
BAO Ying1; LI Ze-qing2; WEI Lin-yan1; CHEN Chao1; FU Ling1; ZHANG Hong-xin1
(1.Tangshan Normal University, Tangshan 063000, China;2.College of Landscape Architecture, Central South Forestry University of Science and Technology, Changsha 410000, China)
关键词:
月季; 盐胁迫; RcMYB102; 实时荧光定量PCR; 基因表达
Keywords:
Rosa chinensis; salt stress; RcMYB102; real-time fluorescence quantitative PCR; gene expression
分类号:
S685.12
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.023
文献标志码:
A
摘要:
为研究月季MYB类转录因子基因RcMYB102的生物学功能，以月季品种月月粉为材料，利用生物信息学分析和实时荧光定量PCR技术研究RcMYB102基因在盐处理、激素处理、盐加激素共处理下不同组织不同处理时间的表达特性。克隆得到一个MYB类转录因子基因，命名为RcMYB102，该基因全长为1 492 bp，开放阅读框(ORF)为1 086 bp，编码362个氨基酸。序列比对发现，RcMYB102在N端具有保守的R2R3-MYB结构域。系统进化树分析结果表明RcMYB102与梅花PmMYB6、桃PpMYB6、甜樱桃PaMYB102、苹果MdMYB74、枇杷EjMYB4处于同一分支，属于R2R3-MYB类转录因子。实时荧光定量PCR结果表明：在盐胁迫下，外施水杨酸(SA)和茉莉酸甲酯(MeJA)可诱导RcMYB102的表达，且均高于单盐胁迫处理和激素处理。上述结果表明，RcMYB102可能与SA和MeJA信号转导途径相关，推测其在月季响应盐胁迫过程中起到一定的作用。
Abstract:
In order to predict the biological function of transcription factor gene RcMYB102 in Rosa chinensis, the Rosa chinensis Old Blush was used as the material, the bioinformatics analysis of RcMYB102 was conducted and its expression characteristics in diverse tissues under salt, hormone, and salt combined with hormore treatments were analyzed by real-time fluorescence quantitative PCR. A MYB transcription factor gene in R. chinensis was obtained and named RcMYB102. The results of bioinformatic analysis showed that the full length of RcMYB102 gene was 1 492 bp, and the open reading frame(ORF) of RcMYB102 was 1 086 bp. Moreover, RcMYB102 gene encoded 362 amino acids. Results of sequence clignment revealed that RcMYB102 had a conserved R2R3-MYB domain at the N-terminal. Phylogenic tree analysis showed that RcMYB102 was clustered with PmMYB6, PpMYB6, PaMYB102, MdMYB74 and EjMYB4, and belonged to the R2R3-MYB transcription factor. Real-time PCR results indicated that, under salt stress, RcMYB102 was significantly up-regulated by salicylicacid (SA) and methyl jasmonate (MeJA), and the expression levels were higher than those under salt stress treatment and exogenous hormone treatment. These results indicate that RcMYB102 may be related to signal transduction pathways of SA and MeJA, suggesting that RcMYB102 may play a role in response to the salt stress in R. chinensis.

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备注/Memo

备注/Memo:
收稿日期：2020-06-05基金项目：河北省高等学校科学技术研究项目(BJ2017102)；中国博士后科学基金项目(2019M652816)；唐山师范学院博士基金项目(2014A06)作者简介：包颖(1983-)，女，河北廊坊人，博士，讲师，研究方向为月季遗传改良与分子生物学。(E-mail) baoying090924@126.com
更新日期/Last Update: 2021-01-15