[1]朱璐,李淑顺,闻婧,等.鸡爪槭ApMYB306基因克隆及功能分析[J].江苏农业学报,2020,(06):1512-1520.[doi:doi:10.3969/j.issn.1000-4440.2020.06.022]
　ZHU Lu,LI Shu-shun,WEN Jing,et al.Cloning and functional analysis of ApMYB306 gene in Acer palmatum[J].,2020,(06):1512-1520.[doi:doi:10.3969/j.issn.1000-4440.2020.06.022]
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鸡爪槭ApMYB306基因克隆及功能分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1512-1520
栏目:
园艺
出版日期:
2020-12-31

文章信息/Info

Title:
Cloning and functional analysis of ApMYB306 gene in Acer palmatum
作者:
朱璐1; 李淑顺1; 闻婧1; 马秋月1; 颜坤元1; 任杰2; 李倩中1
(1.江苏省农业科学院休闲农业研究所，江苏南京210014；2.安徽省农业科学院农业工程研究所，安徽合肥230031)
Author(s):
ZHU Lu1; LI Shu-shun1; WEN Jing1; MA Qiu-yue1; YAN Kun-yuan1; REN Jie2; LI Qian-zhong1
(1.Institute of Leisure Agriculture， Jiangsu Academy of Agricultural Sciences, Nanjing 210014， China;2.Institute of Agricultural Engineering, Anhui Academy of Agricultural Sciences, Hefei 230031, China)
关键词:
鸡爪槭; ApMYB306基因; 抗逆性
Keywords:
Acer palmatum; ApMYB306 gene; stress resistance
分类号:
S687
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.022
文献标志码:
A
摘要:
采用基因克隆的方法获得鸡爪槭金陵黄枫R2R3-MYB转录因子基因，经同源比对和生物信息学分析将其命名为ApMYB306。通过组织表达特性分析发现ApMYB306在茎中表达量最高，叶中表达量次之，根中表达量最低。在洋葱表皮细胞中瞬时表达分析结果显示ApMYB306蛋白定位于细胞核中。ApMYB306对于高温、低温、干旱和盐胁迫均有明显的响应，受胁迫诱导后表达量显著上升。冷冻处理后，转ApMYB306基因株系成活率较野生型升高。ApMYB306超表达拟南芥株系较野生型开花延迟，表明ApMYB306可能通过抑制拟南芥FT基因启动子的表达调控花期。
Abstract:
In this study, the R2R3-MYB transcription factor gene was obtained from Acer palmatum Jinlinghuangfeng by gene cloning and named ApMYB306 via homology comparison and bioinformatics analysis. Through the analysis of tissue expression characteristics, it was found that the expression level of ApMYB306 was the highest in the stem, followed by leaves, and the expression level was lowest in the roots. The results of transient expression analysis in onion epidermal cells showed that ApMYB306 protein was localized in the nucleus. ApMYB306 had obvious response to high temperature, low temperature, drought and salt stress, and its expression level increased significantly after induction. After freezing treatment, the survival rate of ApMYB306 transgenic lines was higher than that of the wild-type lines. The flowering time of ApMYB306 overexpressing Arabidopsis lines was later than that of the wild-type lines, which indicated that ApMYB306 might regulate the flowering time by inhibiting the expression of the FT gene promoter.

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备注/Memo

备注/Memo:
收稿日期：2020-09-07基金项目：国家自然科学基金项目(31700628)；江苏省自然科学基金项目(BK20170602)；江苏省农业科技自主创新基金项目[CX(17)1004]作者简介：朱璐(1987-)，女，山东淄博人，博士，助理研究员，研究方向为鸡爪槭遗传育种。(E-mail)luzhu@jaas.ac.cn通讯作者：李倩中，(E-mail)qianzhongli@jaas.ac.cn
更新日期/Last Update: 2021-01-15