[1]王永竹,徐为民,杨康,等.H2S对硒胁迫下不结球白菜体内BrPAO1/H2O2的调控作用[J].江苏农业学报,2019,(04):887-895.[doi:doi:10.3969/j.issn.1000-4440.2019.04.020]
　WANG Yong zhu,XU Wei min,YANG Kang,et al.Regulation of H2S on BrPAO1/H2O2 under selenium stress in Brassica rapa[J].,2019,(04):887-895.[doi:doi:10.3969/j.issn.1000-4440.2019.04.020]
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H2S对硒胁迫下不结球白菜体内BrPAO1/H2O2的调控作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年04期
页码:
887-895
栏目:
园艺
出版日期:
2019-08-31

文章信息/Info

Title:
Regulation of H2S on BrPAO1/H2O2 under selenium stress in Brassica rapa
作者:
王永竹1; 徐为民2; 杨康1; 许俊丽1; 陈健2; 杨立飞1
（1.南京农业大学园艺学院，江苏南京210095；2.江苏省农业科学院农产品质量安全与营养研究所，江苏南京210014 ）
Author(s):
WANG Yongzhu1; XU Weimin2; YANG Kang1; XU Junli1; CHEN Jian2; YANG Lifei1
（1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Food Quality and Detection, Jiangsu Academy of Agriculture Science, Nanjing 210014, China）
关键词:
不结球白菜; 硒; 硫化氢; 过氧化氢; BrPAO1
Keywords:
Brassica rapa; selenium; hydrogen sulfide; hydrogen peroxide; BrPAO1
分类号:
S634.3
DOI:
doi:10.3969/j.issn.1000-4440.2019.04.020
文献标志码:
A
摘要:
土壤硒（Se）污染已受到越来越多的关注。Se胁迫通常破坏植物体内的抗氧化系统，进而导致H2O2被动累积，多胺氧化酶（PAO）代谢多胺产生H2O2是植物体内活性氧的重要来源之一。本研究以不结球白菜（Brassica rape.ssp.chinensis）幼苗根为研究材料，采用多种生理生化手段，研究了信号分子硫化氢（H2S）对Se胁迫下BrPAO1/H2O2系统的调控方式。结果表明，（1）Se胁迫导致根内PAO活性和H2O2含量显著上升，而采用PAO活性抑制剂（GZT）可显著抑制PAO活性并降低H2O2含量，表明在Se胁迫下导致了依赖于PAO的H2O2产生。（2）从不结球白菜体内克隆了1个编码PAO蛋白的基因BrPAO1,将BrPAO1在烟草叶片中瞬时表达，证明编码蛋白具备产生H2O2的能力；Se胁迫可显著诱导根内BrPAO1的表达。（3）Se胁迫诱导根内H2S合成相关基因（BrLCD1～BrLCD10和BrDCD1、BrDCD2）表达上调，内源H2S含量显著提高；H2S供体（NaHS）处理可进一步提高Se胁迫下根内源H2S水平，而H2S的清除剂（HT）处理可显著降低Se胁迫下根内源H2S水平。（4）NaHS处理可显著抑制Se胁迫下根内BrPAO1基因的表达、PAO活性、H2O2含量，而HT处理则呈现出相反的作用效果。表明，Se胁迫可导致BrPAO1基因表达上调，进而提高PAO活性并产生过量H2O2，而内源H2S则参与调控这一生理过程。
Abstract:
Selenium (Se) contamination in soil has attracted more and more attention. Se stress always results in the passive accumulation of H2O2 by destroying antioxidant system in plants. Polyamine metabolism facilitated by polyamine oxidase (PAO) can produce H2O2, which is one of the important sources of reactive oxygen species (ROS) in plants. In this study, physiologicalbiochemical approaches were applied to investigate the regulation of BrPAO1/H2O2 by hydrogen sulfide (H2S) in the root of Brassica rapa seedlings under Se stress. The results showed that PAO activity and H2O2 content in root increased significantly under Se stress. Treatment with PAO activity inhibitor (GZT) resulted in significant decrease in PAO activity and H2O2 content in Setreated root, suggesting that Se stress triggered PAOdependent H2O2 production in root. A BrPAO1 gene encoding PAO was cloned from B. rapa. The capability of BrPAO1 in H2O2 production was confirmed by transiently expressing BrPAO1 in tobacco leaves. Se stress significantly upregulated the expression of BrPAO1 in root. Se stress resulted in remarkable increase in the expression of genes related to H2S synthesis and endogenous H2S level in root. Treatment with H2S donor (NaHS) enhanced endogenous H2S level in root under Se stress, while treatment with H2S scavenger (HT) led to the decrease in endogenous H2S level in Setreated root. Treatment with NaHS resulted in significant decrease in the expression level of BrPAO1, PAO activity, and H2O2 content, while treatment with HT showed adverse effects. These results suggested that Se stress triggered PAOdependent H2O2 production by upregulating the expression of BrPAO1 in root. And endogenous H2S was involved in the regulation of BrPAO1/H2O2 system.

参考文献/References:

[1]CHEN Y, MO H Z, ZHNG M Y, et al. Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa [J]. PLoS ONE, 2014, 9(10): e110904.
[2]PANKIEWICZ U, SUJKA M, KOWALSKI R, et al. Effect of pulsed electric fields (PEF) on accumulation of selenium and zinc ions in Saccharomyces cerevisiae cells [J]. Food Chemistry, 2017, 221: 1361.
[3]ZHU Y G, PILONSMITS E A H, ZHAO F J, et al. Selenium in higher plants: understanding mechanisms for biofortification and phytoremediation [J]. Trends in Plant Science, 2009, 14(8): 436-442.
［4]陈雪,沈方科,梁欢婷,等. 外源施硒措施对水稻产量品质及植株硒分布的影响［J]. 南方农业学报,2017,48(1):46-50.
[5]GEOFFROY L, GILBIN R, SIMON O, et al. Effect of selenate on growth and photosynthesis of Chlamydomonas reinhardtii [J]. Aquatic Toxicology, 2007, 83(2): 149-158.
[6]陈健，陈义，杨立飞，等. 不结球白菜体内硒累积量与生理损伤指标的相关性[J]. 江苏农业学报, 2016, 32(2): 424-429.
[7]KOLBERT Z, LEHOTAI N, MOLNAR A, et al. ‘The roots’ of selenium toxicity: a new concept[J]. Plant Signaling & Behavior, 2016, 11(10): 16-20.
[8]WINTERBOURN C C. The biological chemistry of hydrogen peroxide [J]. Methods in Enzymology, 2013, 528: 3-25.
[9]BIENERT G P, CHAUMONT F. Aquaporinfacilitated transmembrane diffusion of hydrogen peroxide [J]. Biochimica et Biophysica Acta (BBA)  General Subjects, 2014, 1840(5): 1596-1604.
[10]LEHOTAI N, LYUBENOVA L, SCHDER P, et al. Nitrooxidative stress contributes to selenite toxicity in pea ( Pisum sativum, L.) [J]. Plant & Soil, 2016, 400(1/2): 1-16.
[11]KUSANO T, BERBERICH T, TATEDA C, et al. Polyamines: essential factors for growth and survival [J]. Planta, 2008, 228(3): 367-381.
[12]MOSCHOU P N, SANMARTIN M, ANDRIOPOULOU A H, et al. Bridging the gap between plant and mammalian polyamine catabolism: a novel peroxisomal polyamine oxidase responsible for a full backconversion pathway in Arabidopsis[J]. Plant Physiology, 2008, 147(4): 1845-1857.
[13]WU J, SHANG Z, WU J, et al. Spermidine oxidasederived H2O2 regulates pollen plasma membrane hyperpolarizationactivated Ca2+permeable channels and pollen tube growth [J]. Plant Journal for Cell & Molecular Biology, 2010, 63(6): 1042.
[14]YODA H, FUJIMURA K, TAKAHASHI H, et al. Polyamines as a common source of hydrogen peroxide in host and nonhost hypersensitive response during pathogen infection[J]. Plant Molecular Biology, 2009, 70(1/2): 103-112.
[15]MO H, WANG X, ZHANG Y, et al. Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae [J]. Plant Journal, 2015, 83(6): 962-975.
[16]LI Z G, MIN X, ZHOU Z H. Hydrogen sulfide: a signal molecule in plant crossadaptation [J]. Frontiers in Plant Science, 2016, 7: 1621.
[17]ZHANG H, HU L Y, HU K D, et al. Hydrogen sulfide promotes wheat seed germination and alleviates oxidative damage against copper stress [J]. Acta Botanica Sinica, 2008, 50(12): 1518-1529.
[18]ZHANG H, HU L Y, LI P, et al. Hydrogen sulfide alleviated chromium toxicity in wheat [J]. Biologia Plantarum, 2010, 54(4): 743-747.
[19]CHEN J, WANG W H, WU F H, et al. Hydrogen sulfide alleviates aluminum toxicity in barley seedlings [J]. Plant & Soil, 2013, 362(1/2): 301-318.
[20]QIAO Z, JING T, LIU Z, et al. H2S acting as a downstream signaling molecule of SA regulates Cd tolerance in Arabidopsis [J]. Plant & Soil, 2015, 393(1/2): 1-10.
[21]SHI H, YE Y, CHAN Z. Nitric oxideactivated hydrogen sulfide is essential for cadmium stress response in bermudagrass (Cynodon dactylon L. Pers.) [J]. Plant Physiology and Biochemistry, 2014, 74: 99-107.
[22]CHEN B X, LI W Y, GAO Y T, et al. Involvement of polyamine oxidaseproduced hydrogen peroxide during coleorhizalimited germination of rice seeds [J]. Frontiers in Plant Science, 2016, 7(401): 1219.
[23]汪天,郭世荣,刘俊,等.多胺氧化酶检测方法的改进及其在低氧水培黄瓜根系中的应用[J]. 植物生理学报, 2004, 40(3):358-360.
[24]陈义. 不结球白菜硒胁迫的生理响应及内源NO与H2S介导其胁迫的分子机制[D]. 南京：南京农业大学, 2015.
[25]FINCATO P, MOSCHOU P N, SPEDALETTI V, et al. Functional diversity inside the Arabidopsis polyamine oxidase gene family [J].Journal of Experimental Botany, 2011, 62: 1155-1168.
[26]WIMALASEKERA R, TEBARTZ F, SCHERER G F. Polyamines, polyamine oxidases and nitric oxide in development, abiotic and biotic stresses.[J]. Plant Science An International Journal of Experimental Plant Biology, 2011, 181(5): 593-603.
[27]GILL S S, TUTEJA N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants [J]. Plant Physiology and Biochemistry, 2010, 48: 909-930.
[28]GEMES K, KIM Y J, PARK K Y, et al. An NADPHoxidase/polyamine oxidase feedback loop controls oxidative burst under salinity [J]. Plant Physiology, 2016, 172: 1418-1431.
[29]JIN Z, PEI Y. Physiological implications of hydrogen sulfide in plants: pleasant exploration behind its unpleasant odour [J]. Oxidative Medicine and Cellular Longevity, 2015, 12: 1-6.
[30]JU Y, FU M, STOKES E, et al. H2Smediated protein SSulfhydration: a prediction for its formation and regulation [J]. Molecules, 2017, 22: 1334.

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备注/Memo

备注/Memo:
收稿日期：2018-12-07 基金项目：中央高校基本科研业务费专项(KYZ201830)；国家自然科学青年基金项目(31401857)；国家自然科学基金项目（31771705） 作者简介：王永竹（1992-），女，河北衡水人，硕士研究生，主要从事蔬菜生理与生物技术研究。（E-mail）1343138420@qq.com 通讯作者：杨立飞，（E-mail）Ify@njau.edu.cn
更新日期/Last Update: 2019-08-31