[1]陈 健,陈 义,杨立飞,等.不结球白菜体内硒累积量与生理损伤指标的相关性[J].江苏农业学报,2016,(02):424-429.[doi:10.3969/j.issn.1000-4440.2016.02.028]
　CHEN Jian,CHEN Yi,YANG Li-fei,et al.Correlation between selenium accumulation and physiological injury in Brassica rapa[J].,2016,(02):424-429.[doi:10.3969/j.issn.1000-4440.2016.02.028]
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不结球白菜体内硒累积量与生理损伤指标的相关性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
424-429
栏目:
园艺
出版日期:
2016-03-20

文章信息/Info

Title:
Correlation between selenium accumulation and physiological injury in Brassica rapa
作者:
陈 健1;  陈 义2;  杨立飞2;  徐为民3
1.江苏省农业科学院食品质量安全与检测研究所,江苏 南京 210014; 2.南京农业大学园艺学院,江苏 南京 210095; 3.江苏省农业科学院中心实验室,江苏 南京 210014
Author(s):
CHEN Jian1;  CHEN Yi2;  YANG Li-fei2;  XU Wei-min3
1.Institute of Food Quality Safety and Detection,Jiangsu Academy of Agricultural Sciences,Nanjing 20014,China; 2.College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China; 3.Central Laboratory,Jiangsu Academy of Agricultural Sciences,Nanjing 20014,China
关键词:
不结球白菜;  硒累积;  氧化损伤;  叶绿素含量;  丙二醛含量
Keywords:
Brassica rapa;  selenium accumulation;  oxidative injury;  chlorophyll;  MDA content
分类号:
S636.9
DOI:
10.3969/j.issn.1000-4440.2016.02.028
文献标志码:
A
摘要:
为了研究累积到作物体内的过量硒抑制作物生长的机理,以不结球白菜(Brassica rapa)为材料,分析了亚硒酸钠(Na2SeO3)处理不结球白菜根后,不结球白菜地上部可食部分硒累积量与生理损伤指标变化的相关性。结果显示:随着处理液中硒浓度的升高,不结球白菜地上部和地下部的硒累积量显著上升,且两部分几乎累积等量的硒; 硒处理抑制不结球白菜生长,地上部硒累积量与鲜质量显著负相关; 硒处理刺激不结球白菜地上部丙二醛的产生,地上部硒累积量与丙二醛含量显著正相关,而硒处理下丙二醛含量与鲜质量显著负相关; 低浓度硒处理不影响不结球白菜叶绿素含量,但高浓度硒处理显著降低不结球白菜叶绿素含量,地上部硒累积量与叶绿素含量显著负相关,然而硒处理下叶绿素含量与鲜质量无显著相关性。表明,高浓度硒处理能够导致硒在不结球白菜体内大量累积,进而抑制不结球白菜生长。其中的主要原因可能是硒累积到不结球白菜体内导致的氧化损伤,而非叶绿素含量下降。
Abstract:
To study the mechanism of growth inhibition induced by selenium(Se)accumulation in plants, the correlation between the Se concentration and physiological injury in the edible parts(shoots)of Brassica rapa with root treatment of Na2SeO3 were analyzed. The Se accumulations in both shoots and roots increased significantly,and the amount was close in both parts. Treatment with Se significantly inhibited the growth of B.rapa, and a significantly negative correlation was found between Se concentration in shoots and the fresh weight of shoots. Treatment with Se remarkably induced the production of MDA in the shoots of B. rapa, and Se concentration in shoots was positively correlated with MDA concentration in shoots.And the MDA concentration in shoots was negatively correlated with the fresh weight of shoots under Se treatment. Treatment with Se at low concentrations didn't change the chlorophyll content while Se at high concentrations remarkably induced the decrease in chlorophyll content. A significantly positive correlation between Se concentration in shoots and chlorophyll content was presented,whereas no significant correlation between chlorophyll content and the fresh weight of shoots was observed.In conclusion, high concentration of Se treatment could result in the considerable accumulation of Se in B. rapa,which further led to the growth inhibition of B. rapa.And the reason could be mainly attributed to the oxidative injury instead of the decrease in chlorophyll content in B. rapa.

参考文献/References:

[1] STEINBRENNER H,SIES H.Protection against reactive oxygen species by selenoproteins[J].Biochimica et Biophysica Acta(BBA)-General Subjects,2009,1790(11):1478-1485.
[2] KöHRLE J,GÄRTNER R.Selenium and thyroid[J].Best practice & Research Clinical Endocrinology & Metabolism,2009,23(6):815-827.
[3] SEO Y R,SWEENEY C,SMITH M L.Selenomethionine INDUCTION of DNA repair response in human fibroblasts[J].Oncogene,2002,21(23):3663-3669.
[4] BROOME C S,MCARDLE F,KYLE J A,et al.An increase in selenium intake improves immune function and poliovirus handling in adults with marginal selenium status[J].The American Journal of Clinical Nutrition,2004,80(1):154-162.
[5] 邹 慧,劳雪芬,李丹丹,等. 富硒女贞子对乳腺上皮细胞增殖活性的影响[J]. 江苏农业科学,2015,43(5): 212-216.
[6] 王玉凤,徐 暄,孙其文.硒浸种对番茄种子萌发的影响[J].湖北农业科学,2009,10:2461-2463.
[7] 王淑珍,赵喜茹,王喜枝,等.叶面喷施亚硒酸钠对大蒜光合作用及产量品质的影响[J].中国农学通报,2008,24(4):294-297.
[8] KUMAR M,BIJO A,BAGHEL R S,et al.Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation[J].Plant Physiology and Biochemistry,2012,51:129-138.
[9] DJANAGUIRAMAN M,PRASAD P,SEPPANEN M.Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhancing antioxidant defense system[J].Plant Physiology and Biochemistry,2010,48(12):999-1007.
[10] KAVLAK G,GRAEDEL T E.Global anthropogenic selenium cycles for 1940-2010[J].Resources,Conservation and Recycling,2013,73:17-22.
[11] HUANG S,HUA M,FENG J,et al.Assessment of selenium pollution in agricultural soils in the Xuzhou district,northwest Jiangsu,China[J].Journal of Environmental Sciences,2009,21:481-487.
[12] PACIOLLA C,D E LEONARDIS S,DIPIERRO S.Effects of selenite and selenate on the antioxidant systems in Senecioscandens L.[J].Plant Biosystems,2011,145:253-259.
[12] AGGARWAL M,SHARMA S,KAUR N,et al.Exogenous proline application reduces phytotoxic effects of selenium by minimising oxidative stress and improves growth in bean(Phaseolus vulgaris)seedlings[J].Biological Trace Element Research,2011,140:354-367.
[14] Accumulation of selenium in Ulva sp.and effects on morphology,ultrastructure and antioxidant enzymes and metabolites[J].Aquatic Toxicology,2012,122-123:222-231.
[15] LEHOTAI N,KOLBERT Z,PETÖ A,et al.Selenite-induced ormonal and signalling mechanisms during root growth of Arabidopsis thaliana[J].Journal of Experimental Botany,2012,63:5677-5687.
[16] 陈 义,陈 健,吕文静,等. 硒通过调控小白菜幼苗根内NO与H2S信号诱导BrRbohG1/BrRbohG2 表达[J]. 江苏农业学报,2015, 31(1): 149-157.
[17] CHEN Y,MO H Z,ZHENG M Y,et al.Selenium inhibits root elongation by repressing the generation of endogenous hydrogen sulfide in Brassica rapa[J].PLoS ONE,2014,9(10):e110904.
[18] CHEN Y,MO H Z,HU L B,et al.The endogenous nitric oxide mediates selenium-induced phytotoxicity by promoting ROS generation in Brassica rapa[J].PLoS ONE,2014,9(10):e110901.
[19] SILVA M,ARRUDA M.Identification of selenium in the leaf protein of sunflowers by a combination of 2D-PAGE and laser ablation ICP-MS[J].Microchimica Acta,2012,176:131-136.
[20] FANG Y,CATRON B,ZHANG Y,et al.Distribution and in vitro availability of selenium in selenium-containing storage protein from selenium-enriched rice utilizing optimized extraction[J].Journal of Agricultural and Food Chemistry,2010,58:9731-9738.
[21] McCORD J M,FRIDOVICH I.Superoxide dismutase an enzymic function for erythrocuprein(hemocuprein)[J].Journal of Biological Chemistry,1969,244(22):6049-6055.
[22] 刘绚霞,董振生,刘创社,等.油菜叶绿素提取方法的研究[J].中国农学通报,2004,20(4):62-63.
[23] HUANG S,HUA M,FENG J,et al.Assessment of selenium pollution in agricultural soils in the Xuzhou District,Northwest Jiangsu,China[J].Journal of Environmental Sciences,2009,21:481-487.
[24] KEUNEN E,REMANS T,BOHLER S,et al.Metal-induced oxidative stress and plant mitochondria[J].International Journal of Molecular Sciences,2011,12:6894-6918.
[25] WHITE P,BOWEN H,PARMAGURU P,et al.Interactions between selenium and sulphur nutrition in Arabidopsis thaliana[J].Journal of Experimental Botany,2004,55(404):1927-1937.
[26] ZHAO X Q,MITANI N,YAMAJI N,et al.Involvement of silicon influx transporter OsNIP2; 1 in selenite uptake in rice[J].Plant Physiology,2010,153:1871-1877.
[27] JOHANSSON L,GAFVELIN G,ARNER E S J.Selenocysteine in proteins—properties and biotechnological use[J].Biochim Biophys Acta(BBA)—General Subjects,2005,1726:1-13.
[28] GILL S S,TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry,2010,48:909-930.
[29] REEVES M A,HOFFMAN P R.The human selenoproteome:recent insights into functions and regulation[J].Cellular and Molecular Life Sciences,2009,66(15):2457-2478.
[30] ROCHER C,LALANNE J L,CHAUDIERE J.Purification and properties of a recombinant sulfur analog of murine selenium-glutathione peroxidase[J].European Journal of Biochemistry,1992,205:955-960.

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备注/Memo

备注/Memo:
收稿日期:2015-07-10 基金项目:国家自然科学基金项目(31401857) 作者简介:陈 健(1982-),男,山东潍坊人,博士,副研究员,研究方向为植物逆境生理与生化。(E-mail)chenjian@jaas.ac.cn;(Tel)18761881765 通讯作者:徐为民,(E-mail)weimin0518@sina.com
更新日期/Last Update: 2016-03-20