[1]姜子义,李碧,蔡瑶,等.猪伪狂犬病毒野毒株与疫苗株的多重PCR鉴别[J].江苏农业学报,2018,(06):1307-1311.[doi:doi:10.3969/j.issn.1000-4440.2018.06.015]
　JIANG Zi-yi,LI Bi,CAI Yao,et al.Multiplex PCR identification of pseudorabies vaccine strains and wild viruses[J].,2018,(06):1307-1311.[doi:doi:10.3969/j.issn.1000-4440.2018.06.015]
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猪伪狂犬病毒野毒株与疫苗株的多重PCR鉴别()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年06期
页码:
1307-1311
栏目:
畜牧兽医·水产养殖
出版日期:
2018-12-25

文章信息/Info

Title:
Multiplex PCR identification of pseudorabies vaccine strains and wild viruses
作者:
姜子义1; 李碧1; 蔡瑶1; 颜久淇1; 龚双燕1; 樊毅1; 黄剑波1; 朱玲1; 2; 徐志文1; 2
（1.四川农业大学动物医学院，四川成都611130；2.四川农业大学动物疫病与人类健康四川省重点实验室，四川成都611130）
Author(s):
JIANG Zi-yi1; LI Bi1; CAI Yao1; YAN Jiu-qi1; GONG Shuang-yan1; FAN Yi1; HUANG Jian-bo1; ZHU Ling1; 2; XU Zhi-wen1; 2
(1.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China)
关键词:
PRV; 检测; 多重PCR; 疫苗株; 变异株
Keywords:
pseudorabies virus(PRV); diagnosis; multiplex PCR; vaccine strain; wild virus
分类号:
S858.282
DOI:
doi:10.3969/j.issn.1000-4440.2018.06.015
文献标志码:
A
摘要:
为建立一种快速鉴别猪伪狂犬病毒野毒株与疫苗株的方法，根据猪伪狂犬病毒（PRV）全基因序列，并参照SA215、Bartha-K61疫苗株基因缺失位点特征，在单一检测野毒株与疫苗株的基础上，针对gE、gB和TK 基因设计引物，首次建立了同时检测猪伪狂犬病毒野毒株与疫苗株的多重PCR检测方法。通过一次PCR检测能区分感染野毒株、疫苗株SA215和Bartha-K61，且与猪圆环Ⅱ型病毒（PCV-2）、细小病毒（PPV）、猪繁殖与呼吸综合征病毒（PRRSV）和猪瘟病毒（CSFV）无交叉反应。多次试验证明该方法简单、可靠且敏感度高，可用于科学研究和临床诊断等方面。
Abstract:
In order to establish a diagnostic method for rapid identification of swine pseudorabies virus (PRV) wild virus infection and vaccine strains, this study was developed based on the whole gene sequence of PRV and the characteristics of gene deletion sites of SA215 and Bartha-K61 vaccine strains. On the basis of single detection of vaccine virus and wild virus, primers designed for the three genes of gE, gB and TK were used to establish a multiplex PCR assay for the simultaneous detection of wild virus infections and vaccine strains in pigs. The infection of wild-type virus, vaccine strains SA215 and Bartha-K61 were distinguished by the PCR assay once and there was no cross reaction with porcine circovirus type 2(PCV-2), porcine parvovirus(PPV), porcine reproductive and respiratory syndrome virus(PRRSV) and classical swine fever virus(CSFV). Several experiments have proved that the method is simple, reliable and highly sensitive. It can be used in scientific research, clinical diagnosis and other aspects.

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备注/Memo

备注/Memo:
收稿日期：2018-01-31 基金项目：国家科技计划(科技支撑-子课题)项目(2015BAD12B00-2) ; 四川省科技支撑计划项目(2017NZ0038) 作者简介：姜子义（1992-），男，湖南株洲人，硕士研究生，主要从事病原分子生物学研究，(E-mail)jiangziyi_2013@163.com 通讯作者：徐志文，(E-mail)abtcxzw@126.com
更新日期/Last Update: 2018-12-28