[1]王永娟,左伟勇,朱善元,等.鸭呼肠孤病毒的RT-PCR检测方法的建立[J].江苏农业学报,2016,(05):1107-1110.[doi:10.3969/j.issn.1000-4440.2016.05.024]
 WANG Yong-juan,ZUO Wei-yong,ZHU Shan-yuan,et al.Establishment of a RT-PCR method for duck reovirus detection[J].,2016,(05):1107-1110.[doi:10.3969/j.issn.1000-4440.2016.05.024]
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鸭呼肠孤病毒的RT-PCR检测方法的建立()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年05期
页码:
1107-1110
栏目:
畜牧兽医·水产养殖
出版日期:
2016-11-22

文章信息/Info

Title:
Establishment of a RT-PCR method for duck reovirus detection
作者:
王永娟左伟勇朱善元王安平吴双洪伟鸣陆辉
江苏农牧科技职业学院/江苏省兽用生物制药高技术研究重点实验室,江苏 泰州 225300
Author(s):
WANG Yong-juanZUO Wei-yongZHU Shan-yuanWANG An-pingWU ShuangHONG Wei-mingLU Hui
Jiangsu Agri-animal Husbandry Vocational College/Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High Tech Research, Taizhou 225300, China
关键词:
呼肠孤病毒RT-PCR检测
Keywords:
duckreovirusRT-PCRdetection
分类号:
S858.322.65+9.4
DOI:
10.3969/j.issn.1000-4440.2016.05.024
文献标志码:
A
摘要:
为快速检测临床鸭呼肠孤病毒的感染情况,根据鸭呼肠孤病毒σC蛋白的基因序列设计1对特异性PCR引物,并以呼肠孤病毒基因组为模板,建立了鸭呼肠孤病毒的特异性RT-PCR检测方法。采集江苏省多地疑似呼肠孤病毒感染病料,提取基因组DNA进行RT-PCR扩增,产物经测序鉴定后判断建立的方法对临床样品的检出率。结果显示,该方法可以特异性扩增鸭呼肠孤病毒σC基因保守区438 bp的序列,与其他鸭病毒无交叉反应,最低可检测1 fg基因组DNA,对于临床样品检出率为100%。该方法可以用于临床快速诊断鸭呼肠孤病毒感染。
Abstract:
To rapidly assess the clinical infection of duck reovirus, a pair of specific primers based on duck reovirus σC protein gene sequence was designed. Using the duck reovirus genome as a template, a specific RT-PCR method was established and applied to the samples collected from ducks infected by suspected reovirus in Jiangsu province. The RT-PCR technique established can specifically amplify the 438-bp sequence of reovirus σC conservative region, and detect the genomic DNA of duck reovirus as low as 1 fg with detection rate of 100%. The RT-PCR can be used for rapid clinical diagnosis of duck reovirus infection.

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备注/Memo

备注/Memo:
收稿日期:2016-06-08 基金项目:江苏省“六大人才”高峰第十二批培养对象支助项目(NY-023、10410015002);江苏省农业科技支撑计划项目(BE2014380);江苏农牧科技学院“凤凰人才工程”项目;江苏农牧科技职业学院重点支持项目(NSFZD1405、NSFPT201510、NSFPT201512) 作者简介:王永娟(1980-),女,江苏海门人,博士,副教授,主要从事动物传染病防治研究,(E-mail)43088591@qq.com 通讯作者:陆辉,(E-mail)smluhui@163.com
更新日期/Last Update: 2016-11-22