[1]陈蓉,应诗家,陈哲,等.两品种鸭下丘脑miRNA的鉴定及其表达验证分析[J].江苏农业学报,2017,(05):1093-1098.[doi:doi:10.3969/j.issn.1000-4440.2017.05.020]
 CHEN Rong,YING Shi-jia,CHEN Zhe,et al.Identification and expression analysis of miRNA in the hypothalamus of two duck breeds[J].,2017,(05):1093-1098.[doi:doi:10.3969/j.issn.1000-4440.2017.05.020]
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两品种鸭下丘脑miRNA的鉴定及其表达验证分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
1093-1098
栏目:
畜牧兽医·水产养殖
出版日期:
2017-10-30

文章信息/Info

Title:
Identification and expression analysis of miRNA in the hypothalamus of two duck breeds
作者:
陈蓉应诗家陈哲于建宁
(江苏省农业科学院畜牧研究所,江苏南京210014)
Author(s):
CHEN RongYING Shi-jiaCHEN ZheYU Jian-ning
(Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
下丘脑miRNA性成熟
Keywords:
duckhypothalamusmiRNAsexual maturity
分类号:
S834.1
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.020
文献标志码:
A
摘要:
为了鉴定与鸭性成熟启动相关的miRNA,根据鸭卵巢组织的发育情况,利用Solexa技术分别对105日龄连城白鸭和樱桃谷鸭的下丘脑组织进行小RNA测序分析。两个小RNA文库共检测到939个成熟miRNA,共有miRNA数量806个。以樱桃谷鸭为对照,两个文库之间总共检测到290个差异表达miRNA,其中上调miRNA 85个,下调miRNA 205个。靶基因预测分析结果表明,较多的靶基因被注释到神经活性配体-受体相互作用、黏着斑、MAPK信号通路、胞吞作用等通路中。定量PCR结果表明,2个差异表达miRNA靶基因的mRNA表达量在两品种鸭下丘脑组织中存在着极显著差异。
Abstract:
In order to identify miRNAs involved in the onset of sexual maturity in duck, small RNA libraries of the hypothalamus of 105-day Liancheng duck and Cherry valley duck were respectively analyzed by Solexa technique according to the development of their ovarian tissues. A total of 939 mature miRNAs were detected from two small RNA libraries and among them 806 miRNAs were co-expressed. A total of 290 differentially expressed miRNAs were detected between the two libraries, with 85 up-regulated miRNAs and 205 down-regulated miRNAs. The results of target gene prediction analysis showed that more target genes were annotated into neuroactive ligand-receptor interaction, focal adhesion, MAPK signaling pathway, and endocytosis so on. The result of quantitative PCR showed that there was a significant difference for the mRNA expression of two differentially expressed miRNA targeted genes in the hypothalamus of two duck breeds.

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备注/Memo

备注/Memo:
收稿日期:2017-03-15 基金项目:江苏省农业自主创新基金项目[CX(14)5032] 作者简介:陈蓉(1985-),女,江苏淮安人,博士,助理研究员,主要从事家禽遗传育种与繁殖方面的研究,(E-mail)chenrong_big@163.com 通讯作者:陈哲,(E-mail)chenzzju@163.com
更新日期/Last Update: 2017-11-03