[1]刘朝茂,李成云.玉米与大豆间作对玉米叶片衰老的影响[J].江苏农业学报,2017,(02):322-326.[doi:doi:10.3969/j.issn.1000-4440.2017.02.013]
 LIU Chao-mao,LI Cheng-yun.Effects of maize/soybean intercropping on maize leaf senescence[J].,2017,(02):322-326.[doi:doi:10.3969/j.issn.1000-4440.2017.02.013]
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玉米与大豆间作对玉米叶片衰老的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年02期
页码:
322-326
栏目:
耕作栽培·资源环境
出版日期:
2017-04-30

文章信息/Info

Title:
Effects of maize/soybean intercropping on maize leaf senescence
作者:
刘朝茂1李成云2
(1.西南林业大学林学院/云南省森林灾害预警与控制重点实验室,云南昆明650224;2.云南农业大学农业生物多样性应用技术国家工程研究中心/教育部农业生物多样性与病虫害控制教育部重点实验室/云南农业大学植物病理重点实验室,云南昆明650201)
Author(s):
LIU Chao-mao1LI Cheng-yun2
(1.Forestry College, Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Southwest Forestry, Kunming 650224, China;2.The National Center for Agricultural Biodiversity, Ministry of Education/Key Laboratory of Agricultural Biodiversity for Plant Disease Management/Key Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China)
关键词:
玉米大豆间作衰老
Keywords:
maizesoybeanintercroppingsenescence
分类号:
S344.2
DOI:
doi:10.3969/j.issn.1000-4440.2017.02.013
文献标志码:
A
摘要:
采用玉米/大豆2∶2间作和玉米净作的小区试验,检测与衰老相关酶在不同生育期的活性,并利用RT-PCR技术,检测不同生育期玉米衰老相关基因的表达情况。结果表明,与净作种植的玉米相比,间作种植的玉米能够维持较高的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,有效增加叶绿素、可溶性糖和可溶性蛋白含量,降低丙二醛(MDA)含量,有效延缓植物衰老。通过分析比较玉米/大豆间作种植与玉米净作种植模式下玉米衰老相关基因的表达情况,发现间作种植模式下玉米SGR1、SGR2、VP14和CC2基因在灌浆期和成熟期的表达量均低于玉米净作种植模式下的,而延缓衰老的基因KN1在玉米/大豆2∶2间作种植模式下的表达量略高于玉米净作种植模式下的,这从基因水平证实了玉米/大豆间作能够延缓玉米植株的衰老。
Abstract:
An experiment consisting of two cropping patterns(2-row maize/2-row soybean intercropping and maize monoculture)was conducted to detect the activities of senescence-related enzymes at different growth stages. Expression of maize senescence-related genes at different growth stages were detected by RT- PCR. Results showed that the enzymes activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were maintained at higher levels, and the contents of chlorophyll, soluble sugar and soluble protein were higher in maize leaves intercropped with soybean, but the content of malondialdehyde (MDA) was decreased, effectively delaying senescence. The expressions of SGR1, SGR2, VP14 and CC2 in intercropped maize at filling and maturity were lower than those in monocultured maize, but the expression of senescence-delaying gene KN1 was higher, confirming that the maize/soybean intercropping could delay maize senescence at gene level.

参考文献/References:

[1]FU Y, DING Y, LIU X, et al. Rice transformation with a senescence-inhibition chimeric gene[J]. Chinese Science Bulletin, 1998, 43(21): 1810-1815.
[2]CLAUSEN S, APEL K. Seasonal changes in the concentration of the major storage protein and its mRNA in xylem ray cells of poplar trees[J]. Plant Molecular Biology, 1991, 17(4): 669-678.
[3]BUCHANAN-WOLLASTON V. The molecular biology of leaf senescence[J].Journal of Experimental Botany, 1997, 48(2): 181-199.
[4]熊军,闫海锋,韦绍丽,等.木薯+花生间作对作物光合特性、农艺性状和产量的影响[J].江苏农业科学,2016,44(6):165-168.
[5]黄鹏,张恩和,柴强. 施氮对新灌区不同间套种植模式产量及茬口养分特性的影响[J]. 草业学报, 2001, 10(1):86-91.
[6]谢利,王燕芳,马超,等. 棉花- 孜然间作模式对土壤微生物数量及酶活性的影响[J]. 江苏农业科学,2015,43(10):103-105.
[7]李隆,杨思存,孙建好,等. 春小麦大豆间作条件下作物养分吸收积累动态的研究[J]. 植物营养与肥料学报, 1999, 5(2):163-171.
[8]杨建波, 彭东海, 覃刘东,等. 低氮条件下间作大豆对宿根蔗内生固氮菌固氮酶活性、氮素积累及产量的影响[J].南方农业学报,2015,46(2):210-215.
[9]张恩和,李玲玲,黄高宝,等. 供肥对小麦间作蚕豆群体产量及根系的调控[J]. 应用生态学报, 2002, 13(8): 939-942.
[10]夏海勇,孟维伟,于丽敏,等. 玉米花生间作在山东地区推广的现状与对策[J].山东农业科学,2015,47(3):121-124.
[11]崔俊明,宋长江,卢道文,等. 不同类型玉米杂交种高矮立体间作种植技术研究[J]. 杂粮作物, 2005, 25(4): 253-257.
[12]刘天学,李潮海,马新明,等. 不同基因型玉米间作对叶片衰老、籽粒产量和品质的影响[J]. 植物生态学报, 2008, 32(4):914-921.
[13]ZHANG X, ZHANG L, DONG F, et al. Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba[J]. Plant Physiology, 2001, 126(4): 1438-1448.
[14]ZHU Y Y, FANG H, WANG Y Y, et al. Panicle blast and canopy moisture in rice cultivar mixtures[J]. Phytopathology, 2005, 95(4): 433-438.
[15]郑炳松. 现代植物生理生化研究技术[M]. 北京:气象出版社, 2006.
[16]高俊凤. 植物生理学实验指导[M]. 北京:高等教育出版社, 2006.
[17]董树刚,吴以平. 植物生理学实验技术[M]. 青岛:中国海洋大学出版社, 2006.
[18]段咏新,宋松泉. 钙对延缓杂交水稻叶片衰老的作用机理[J]. 杂交水稻, 1997, 12(6): 23-25.
[19]杨立桃,赵志辉,丁嘉羽,等. 利用实时荧光定量PCR方法分析转基因水稻外源基因拷贝数[J]. 中国食品卫生杂志, 2005, 17(2): 140-144.
[20]PARK S Y, YU J W, PARK J S, et al. The senescence-induced staygreen protein regulates chlorophyll degradation[J]. The Plant Cell Online, 2007, 19(5): 1649-1664.
[21]BOLDUC N, YILMAZ A, MEJIA-GUERRA M K, et al. Unraveling the KNOTTED1 regulatory network in maize meristems[J]. Genes & Development, 2012, 26(15): 1685-1690.
[22]TAN B C, SCHWARTZ S H, ZEEVAART J A D, et al. Genetic control of abscisic acid biosynthesis in maize[J]. Proceedings of the National Academy of Sciences, 1997, 94(22): 12235-12240.
[23]MASSONNEAU A, CONDAMINE P, WISNIEWSKI J P, et al. Maize cystatins respond to developmental cues, cold stress and drought[J]. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 2005, 1729(3): 186-199.

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备注/Memo

备注/Memo:
收稿日期:2016-11-10 基金项目:国家“973”计划项目(2011CB100403) 作者简介:刘朝茂(1987-),男,云南彝良人,硕士,助理实验师,从事森林病理学研究。(Tel)15288139795; (E-mail)lcm1987swkx@163.com 通讯作者:李成云, (Tel)13708705575; (E-mail)li.chengyun@gmail.com
更新日期/Last Update: 2017-05-02