[1]孟力力,张俊,闻婧.干旱胁迫对彩叶草光合特性及叶片超微结构的影响[J].江苏农业学报,2015,(01):180-185.[doi:10.3969/j.issn.1000-4440.2015.01.028]
　MENG Li-li,ZHANG Jun,WEN Jing.Changes of photosynthetic characteristics of Coleus blumei and mesophyll cell ultrastructure in response to drought stress[J].,2015,(01):180-185.[doi:10.3969/j.issn.1000-4440.2015.01.028]
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干旱胁迫对彩叶草光合特性及叶片超微结构的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
180-185
栏目:
园艺
出版日期:
2015-02-28

文章信息/Info

Title:
Changes of photosynthetic characteristics of Coleus blumei and mesophyll cell ultrastructure in response to drought stress
作者:
孟力力1; 张俊2; 闻婧1
（1.江苏省农业科学院园艺研究所，江苏南京210014；2.江苏省农业科学院蔬菜研究所，江苏南京210014）
Author(s):
MENG Li-li1; ZHANG Jun2; WEN Jing1
（1.Institute of Horticulture，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China；2.Institute of Vegetable Crops，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China）
关键词:
彩叶草; 干旱胁迫; 光合生理特性; 叶片超微结构
Keywords:
Coleus blumei; drought stress; photosynthetic characteristic; mesophyll cell ultrastructure
分类号:
S682.360.1
DOI:
10.3969/j.issn.1000-4440.2015.01.028
文献标志码:
A
摘要:
通过盆栽试验对彩叶草设置4种水分梯度模拟干旱胁迫处理，20 d后测量光合生理指标并观察叶片超微结构的变化。随着干旱胁迫程度的加深，彩叶草株高、茎粗、最大叶面积都呈下降趋势；净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)呈下降趋势，胞间CO2浓度(Ci)呈先降后升趋势，水分利用率(WUE)、气孔限制值(Ls)呈先升后降趋势；光补偿点(LCP)升高,光饱和点(LSP)降低,最大净光合速率(Pmax)和表现光量子效率(AQY)降低，表明干旱胁迫对彩叶草光合系统造成了一定程度的损伤，其光能利用效率下降，出现光抑制现象。轻度和中度干旱胁迫下Pn降低是由气孔限制引起，而在重度干旱胁迫下则由非气孔限制起主要作用。随着干旱胁迫程度的加深，叶绿体结构逐渐受到破坏，基粒片层和基质片层逐渐扭曲。至重度干旱胁迫时，细胞膜边缘模糊甚至部分破裂消失，出现大量嗜锇颗粒；淀粉粒变形糊化，线粒体膜模糊。研究表明，适度的干旱胁迫（土壤含水量为60%65%)可提高彩叶草的水分利用率，辅以适当遮阴可减轻光抑制，提高光合速率。
Abstract:
Four levels of soil moisture treatments were applied to potted Coleus blumei to investigate the influence of drought stress on photosynthetic characteristics and ultrastructure of mesophyll cell. With the aggravation of drought stress, plant height, stem diameter and maximal leaf area reduced, net photosynthesis rate Pn, stomatal conductivity Gs and transpiration rate Tr droped, intercellular CO2 concentration Ci decreased at first and then increased, water use efficiency WUE and stomatal limitation Ls ascended firstly and then descended, light compensation points (LCP) increased, and light points and apparent quantum yield (AQY) dropped. The results indicated that a certain degree of damage to photosynthesis system was induced by drought stress, leading to the reduction of light use efficiency and photoinhibition of photosynthesis. Stomatal limitation played a leading role in the reduction of Pn under light or moderate drought stress which was caused by non-stomatal limitation under serious drought stress. As drought aggravated, chloroplast structures were gradually destroyed, and grana lamellae and stroma lamellae were contorted. Under severe drought stress, the edge of cell membrane got fuzzy, ruptured and dissappeared, a large number of osmiophilic particles appeared, starch grains were out of shape, and mitochodrial membrane got fuzzy. In summary, light drought stress (soil moisture 60%-65%) could enhance the WUE of C. blumei, and reduce photoinhibition and improve the photosynthetic rate when supplemented with shading.

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备注/Memo

备注/Memo:
收稿日期：2014-06-05 基金项目：江苏省农业科技自主创新基金项目[CX(12)5090] 作者简介：孟力力(1982-)，女，山西晋中人，硕士，助理研究员，主要从事设施园艺特殊栽培研究。(Tel)025-84392652；(E-mail)menglili90@163.com
更新日期/Last Update: 2015-02-28