[1]张丽丽,徐碧玉,刘菊华,等.MaASR1基因通过乙烯途径提高拟南芥抗旱性的作用机制[J].江苏农业学报,2018,(03):511-519.[doi:doi:10.3969/j.issn.1000-4440.2018.03.005]
　ZHANG Li-li,XU Bi-yu,LIU Ju-hua,et al.The regulation mechanism of MaASR1 gene for improving the drought resistance of Arabidopsis by ethylene pathway[J].,2018,(03):511-519.[doi:doi:10.3969/j.issn.1000-4440.2018.03.005]
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MaASR1基因通过乙烯途径提高拟南芥抗旱性的作用机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年03期
页码:
511-519
栏目:
遗传育种·生理生化
出版日期:
2018-06-25

文章信息/Info

Title:
The regulation mechanism of MaASR1 gene for improving the drought resistance of Arabidopsis by ethylene pathway
作者:
张丽丽1; 徐碧玉1; 刘菊华1; 贾彩红1; 张建斌1; 金志强1; 2
（1.中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101；2.中国热带农业科学院海口实验站/海南省香蕉遗传育种改良重点实验室,海南海口570102）
Author(s):
ZHANG Li-li1; XU Bi-yu1; LIU Ju-hua1; JIA Cai-hong1; ZHANG Jian-bin1; JIN Zhi-qiang1; 2
(1.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, China;2.Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences/Hainan Provincial Key Laboratory for Genetics and Breeding of Banana, Haikou 570102, China)
关键词:
MaASR1基因; 基因芯片; 干旱胁迫; 乙烯; 实时荧光定量PCR
Keywords:
MaASR1 gene; DNA microarray; drought stress; ethylene; real-time fluorescence quantitative PCR
分类号:
Q786
DOI:
doi:10.3969/j.issn.1000-4440.2018.03.005
文献标志码:
A
摘要:
香蕉MaASR1基因在植物响应逆境胁迫时发挥着重要作用，为了进一步研究MaASR1基因转入拟南芥后增强其抗旱性的分子机制，运用DNA芯片技术来筛选野生型拟南芥和转MaASR1基因拟南芥在不做任何胁迫处理和干旱胁迫处理条件下的差异基因。发现MaASR1提高拟南芥抗旱性与乙烯信号途径有密切的关系，对基因芯片中与乙烯途径相关的上调和下调大于2倍的差异基因进行了荧光定量PCR的验证。结果表明在干旱胁迫条件下，MaASR1的转入通过提高AtACS6和AtACO1的表达水平提高了拟南芥体内的乙烯合成水平。MaASR1的转入可以通过正调控乙烯反应和提高ERF类基因的表达来赋予植物抗旱性。以上结果为解析MaASR1基因作为转录因子通过乙烯途径提高植物抗旱能力的分子机制奠定了基础。
Abstract:
The MaASR1 gene of banana plays an important role in plant response to stress. In order to further study the molecular mechanism of drought resistance for MaASR1 gene in Arabidopsis thaliana, DNA microarray was used to screen the differentially expressed genes under natural and drought stress treatment in wild-type Arabidopsis thaliana and transgenic lines. It was found that MaASR1 could increase drought resistance in Arabidopsis thaliana and had a close relationship with ethylene signaling pathway. The genes related to ethylene pathway in the DNA microarray were verified by fluorescence quantitative PCR. The results showed that MaASR1 could improve the ethylene synthesis level in vivo by raising the expression level of ACS6 and ACO1 in Arabidopsis under the treatment of drought stress. The transfer of MaASR1 could confer drought resistance to Arabidopsis transgenic plants by positively regulating the ethylene response and increasing the gene expression of ERF. The above results lay the foundation for the analysis of the molecular mechanism of MaASR1 gene as a transcription factor to improve plant drought resistance through ethylene pathway.

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相似文献/References:

[1]张丽丽,徐碧玉,刘菊华,等.香蕉MaASR1基因的生物信息学分析及原核表达[J].江苏农业学报,2017,(05):1129.[doi:doi:10.3969/j.issn.1000-4440.2017.05.026]
　ZHANG Li-li,XU Bi-yu,LIU Ju-hua,et al.Bioinformatics analysis and prokaryotic expression of MaASR1 gene from banana[J].,2017,(03):1129.[doi:doi:10.3969/j.issn.1000-4440.2017.05.026]

备注/Memo

备注/Memo:
收稿日期：2017-10-06 基金项目：海南省重大专项（HNGDpz201502）；转基因生物新品种培育国家科技重大专项 (2016ZX08012005-007)；现代农业产业技术体系建设专项（CARS-31） 作者简介：张丽丽（1984-），女，山东滨州人，博士，助理研究员，主要从事植物分子遗传学研究。（Tel）0898-66894828；（E-mail）zhanglili@itbb.org.cn 通讯作者：金志强，（E-mail）jinzhiqiang@itbb.org.cn
更新日期/Last Update: 2018-07-04