[1]王志科,关伟龙,李志伟,等.外源H2O2对NaCl胁迫下豌豆种子萌发和幼苗抗氧化酶活性的影响[J].江苏农业学报,2024,(01):31-38.[doi:doi:10.3969/j.issn.1000-4440.2024.01.003]
　WANG Zhi-ke,GUAN Wei-long,LI Zhi-wei,et al.Effects of exogenous hydrogen peroxide on pea seed germination and seedling antioxidant enzyme activity under NaCl stress[J].,2024,(01):31-38.[doi:doi:10.3969/j.issn.1000-4440.2024.01.003]
点击复制

外源H2O2对NaCl胁迫下豌豆种子萌发和幼苗抗氧化酶活性的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年01期
页码:
31-38
栏目:
遗传育种·生理生化
出版日期:
2024-01-30

文章信息/Info

Title:
Effects of exogenous hydrogen peroxide on pea seed germination and seedling antioxidant enzyme activity under NaCl stress
作者:
王志科; 关伟龙; 李志伟; 孙于卜
(陇东学院生命科学与技术学院/甘肃省陇东生物资源保护利用与生态修复重点实验室,甘肃庆阳745000)
Author(s):
WANG Zhi-ke; GUAN Wei-long; LI Zhi-wei; SUN Yu-bo
(College of Life Science and Technology, Longdong University/Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration, Qingyang 745000, China)
关键词:
过氧化氢; 盐胁迫; 豌豆种子; 萌发; 生理特性
Keywords:
hydrogen peroxide; salt stress; pea seeds; germination; physiological characteristics
分类号:
Q945.79
DOI:
doi:10.3969/j.issn.1000-4440.2024.01.003
文献标志码:
A
摘要:
以豌豆种子为试验材料,研究不同浓度(0 mmol/L、5 mmol/L、10 mmol/L、20 mmol/L、40 mmol/L和80 mmol/L)外源H2O2对170 mmol/L NaCl溶液处理的豌豆种子萌发、幼苗生长及生理特性的影响。结果表明:与CK相比,170 mmol/L NaCl处理的豌豆种子的萌发和幼苗生长都受到显著抑制,发芽指标和生理指标均显著降低。在170 mmol/L NaCl胁迫下,低浓度外源H2O2浸种处理,能够提高豌豆种子的发芽指标,促进幼苗根和芽的生长,促进脯氨酸和可溶性糖含量的积累,降低丙二醛含量,提高了根和芽中SOD、CAT和POD的活性。说明,外源H2O2能够通过调控渗透调节物质的积累和抗氧化酶活性来提高豌豆耐盐性,其中外源5 mmol/L H2O2处理显著缓解NaCl胁迫对豌豆种子萌发和幼苗生长的毒害,缓解效果最好。
Abstract:
Pea seeds were used as test materials to study the effects of exogenous H2O2 (0 mmol/L, 5 mmol/L, 10 mmol/L, 20 mmol/L, 40 mmol/L, and 80 mmol/L) on the germination, seedling growth and physiological characteristics under 170 mmol/L NaCl treatment. The results showed that compared with CK, seed germination and seedling growth were significantly inhibited under 170 mmol/L NaCl treatment, and both germination index and physiological index were significantly decreased. Under 170 mmol/L NaCl stress, seed soaking with low concentration of exogenous H2O2 could improve the germination index of pea seeds, promote the growth of seedling roots and buds, promote the accumulation of proline and soluble sugar, decrease the content of malondialdehyde, and increase the activities of SOD, CAT and POD in roots and buds. The results showed that exogenous H2O2 could improve salt tolerance of pea by regulating the accumulation of osmotic adjustment substances and antioxidant enzyme activity. Among them, 5 mmol/L H2O2 significantly alleviated the toxicity of NaCl stress on seed germination and seedling growth, and the effect was the best.

参考文献/References:

[1]江杰,王胜. 我国盐碱地成因及改良利用现状[J]. 安徽农业科学,2020,48(13):85-87.
[2]朱建峰,杨秀艳,武海雯,等. 植物种子萌发期耐盐碱性提高技术研究进展[J]. 生物技术通报,2020,36(2):158-168.
[3]班月圆. 盐胁迫对不同地域桑种子萌发的影响及外源GA3和钙的缓解效应研究[D]. 镇江:江苏科技大学,2017.
[4]杨波,何俊瑜,任艳芳,等. 过氧化氢对镉胁迫下水稻种子萌发的缓解效应[J]. 植物生理学报,2018,54(6):1111-1118.
[5]冯潇. 过氧化氢对白沙蒿种子萌发的影响[D]. 兰州:兰州大学,2019.
[6]任艳芳,何俊瑜,杨军,等. 外源H2O2对盐胁迫下小白菜种子萌发和幼苗生理特性的影响[J]. 生态学报,2019,39(20):7745-7756.
[7]孔祥强,罗振,张艳军,等. 过氧化氢预处理促进盐胁迫下棉花种子萌发的效应[C]//棉花学报编辑部. 2015年全国棉花青年学术研讨会论文汇编. 北京:棉花学报编辑部,2015:22-23.
[8]黄珊. 不同处理方法对皂角种子萌发的影响[J]. 防护林科技,2021(3):21-22.
[9]张雅婷. 外源过氧化氢预处理对花生种子低温萌发的影响[D]. 合肥:安徽农业大学,2020.
[10]崔翠,孙建蓉,赵愉风,等. 豌豆嫩尖几个营养品质性状的遗传多样性分析及其综合评价[J]. 植物遗传资源学报,2019,20(4):932-948.
[11]亚秀秀,杨东旭,周桂梅,等. 豌豆种质资源耐盐性的鉴定与评价[J]. 四川农业大学学报,2022,40(4):505-511.
[12]孙冬阳,呼鑫荣,薛文通. 豌豆功效成分及其生理活性的研究进展[J]. 食品工业科技,2019,40(2):316-320.
[13]徐芬芬,叶利民,夏晓蕾. 小白菜抗盐胁迫的根系响应机制[J]. 干旱地区农业研究,2017,35(3):178-181.
[14]仇奕之,李宜珅,杨鹏军,等. 甘氨酸对不同胁迫条件下高山离子芥试管苗的保护作用[J]. 兰州大学学报(自然科学版),2018,54(2):200-207.
[15]达海兰. 盐胁迫对窄叶野豌豆种子萌发的影响[J]. 青海农技推广,2021(1):48-50.
[16]李志萍,张文辉,崔豫川. NaCl和Na2CO3胁迫对栓皮栎种子萌发及幼苗生长的影响[J]. 生态学报,2015,35(3):742-751.
[17]唐鑫华,孟欣,石忆,等. 表油菜素内酯对NaCl胁迫下马铃薯生长和块茎品质的影响[J].中国农业大学学报,2022,27(12):127-137.
[18]朱广龙,宋成钰,于林林,等. 外源生长调节物质对甜高粱种子萌发过程中盐分胁迫的缓解效应及其生理机制[J].作物学报,2018,44(11):1713-1724.
[19]任艳芳,何俊瑜,杨军,等. 外源H2O2对盐胁迫下小白菜种子萌发和幼苗生理特性的影响[J]. 生态学报,2019,39(20):7745-7756.
[20]FANG Y, LI J, JIANG J, et al. Physiological and epigenetic analyses of Brassica napus seed germination in response to salt stress[J]. Acta Physiologiae Plantarum,2017,39(6):128．
[21]NAZIR F, FARIDUDDIN Q, KHAN T A. Hydrogen peroxide as a signalling molecule in plants and its crosstalk with other plant growth regulators under heavy metal stress[J]. Chemosphere,2020,252:1-2.
[22]CERNY M, HABNOV H, BERKA M, et al. Hydrogen peroxide:its role in plant biology and crosstalk with signalling networks[J]. International Journal of Molecular Sciences,2018,19(9):1-30.
[23]XU Q, XU X, ZHAO Y, et al. Salicylic acid,hydrogen peroxide and calcium-induced salinetolerance associated with endogenous hydrogen peroxide homeostasis in naked oat seedlings[J]. Plant Growth Regulation,2008,54(3):249-259．
[24]LI J T, QIU Z B, ZHANG X W, et al. Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress[J]. Acta Physiologiae Plantarum,2011,33(3):835-842．
[25]SACHDEV S, ANSARI S A, ANSARI M I, et al. Abiotic stress and reactive oxygen species:generation,signaling,and defense mechanisms[J]. Antioxidants(Basel),2021,10(2):1-37.
[26]陈贵华,石岭,王萍,等. 外源过氧化氢对苦菜耐盐生理作用的影响[J]. 内蒙古农业大学学报(自然科学版),2017,38(6):8-11.
[27]NADARAJAH K K. ROS homeostasis in abiotic stress tolerance in plants[J]. International Journal of Molecular Sciences,2020,21(15):1-29.
[28]高彦强,颉建明,王成,等. 外源脯氨酸对盐胁迫下芹菜生长及光合特性的影响[J]. 江西农业大学学报,2023,45(2):322-336.
[29]魏茜雅,林欣琪,梁腊梅,等. 褪黑素引发处理提高朝天椒种子萌发及幼苗耐盐性的生理机制[J]. 江苏农业学报,2022,38(6):1637-1647.
[30]李海霞,米银法,陈双臣. 干旱胁迫下6种观赏牡丹生理响应及耐旱性评价[J]. 江苏农业科学,2022,50(7):131-139.
[31]于雄胜,江振岳,张文英,等. 谷子萌发及苗期生理生化指标对铅胁迫的响应[J]. 南方农业学报,2022,53(3):795-802.
[32]里程辉,王杰,王宏,等. 淹水胁迫下不同中间砧对岳华苹果叶片和根系抗氧化酶、非酶类抗氧化物活性的影响[J]. 江苏农业科学,2022,50(11):130-135.
[33]李智博,董世满,曾长英,等. 低温贮藏条件下木薯种茎可溶性糖与干旱胁迫耐受性的相关性研究[J]. 华南农业大学学报,2022,43(4):58-66.
[34]母洪娜,王炜,樊蕾,等. 印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响[J]. 南京林业大学学报(自然科学版),2023,47(2):101-106.
[35]李莉婕,赵泽英,黎瑞君,等. 水氮钾耦合对火龙果产量和品质的调控效应[J]. 南方农业学报,2022,53(3):859-868.
[36]WANI K I, NAEEM M, CASTROVERDE C D M, et al. Molecular mechanisms of nitric oxide(NO) signaling and reactive oxygen species(ROS) homeostasis during abiotic stresses in plants[J]. International Journal of Molecular Sciences,2021,22(17):1-21.
[37]赵海新. 碱胁迫对水稻叶绿素及叶片脯氨酸和可溶性糖含量的影响[J]. 作物杂志,2020(1):98-102.
[38]傅聿青,贾漫丽,王军,等. NaCl胁迫下2个桑树品种的脯氨酸、可溶性糖、可溶性蛋白含量变化研究[J]. 林业与生态科学,2018,33(3):306-310.
[39]王以斌,缪锦来,姜英辉,等. 脯氨酸和可溶性糖在南极冰藻低温适应机制中的作用[J]. 生物技术通报,2016,32(2):198-202.
[40]LIU L, HUANG L, LIN X, et al. Hydrogen peroxide alleviates salinity-induced damage through enhancing proline accumulation in wheat seedlings[J]. Plant Cell Reports,2020,39(5):567-575.
[41]BEN-REJEB K, LEFEBVRE-DE V D, LE-DISQUET I, et al. Hydrogen peroxide produced by NADPH oxidases increases proline accumulation during salt or mannitol stress in Arabidopsis thaliana[J]. New Phytologist,2015,208(4):1138-1148.

相似文献/References:

[1]韩金龙,李慧,蔺经,等.核黄素对盐胁迫下杜梨叶片抗氧化系统的影响[J].江苏农业学报,2015,(04):893.[doi:10.3969/j.issn.1000-4440.2015.04.029]
　HAN Jing-long,LI Hui,LIN Jing,et al.The regulatory role of riboflavin in antioxidant system of Pyrus betulaefolia in response to salt tolerance[J].,2015,(01):893.[doi:10.3969/j.issn.1000-4440.2015.04.029]
[2]安飞飞,简纯平,杨龙,等.木薯幼苗叶绿素含量及光合特性对盐胁迫的响应[J].江苏农业学报,2015,(03):500.[doi:10.3969/j.issn.1000-4440.2015.03.006]
　AN Fei-fei,JIAN Chun-ping,YANG Long,et al.Chlorophyll contents and photosynthetic characteristics of cassava seedlings in response to NaCl stress[J].,2015,(01):500.[doi:10.3969/j.issn.1000-4440.2015.03.006]
[3]刘金龙,辛寒晓,范学明,等.盐胁迫下鱼蛋白多肽对樱桃番茄种子发芽特性的影响[J].江苏农业学报,2017,(03):662.[doi:doi:10.3969/j.issn.1000-4440.2017.03.026]
　LIU Jin-long,XIN Han-xiao,FAN Xue-ming,et al.Effects of fish protein polypeptide on salt-stressed cherry tomato seed germination[J].,2017,(01):662.[doi:doi:10.3969/j.issn.1000-4440.2017.03.026]
[4]田礼欣,李丽杰,刘旋,等.外源海藻糖对盐胁迫下玉米幼苗根系生长及生理特性的影响[J].江苏农业学报,2017,(04):754.[doi:doi:10.3969/j.issn.1000-4440.2017.04.005]
　TIAN Li-xin,LI Li-jie,LIU Xuan,et al.Root growth and physiological characteristics of salt-stressed maize seedlings in response to exogenous trehalose[J].,2017,(01):754.[doi:doi:10.3969/j.issn.1000-4440.2017.04.005]
[5]黄芳,徐珍珍,孟珊,等.盐胁迫下棉花LTR-反转座子的转录激活及在耐盐相关基因发掘中的应用[J].江苏农业学报,2017,(06):1220.[doi:doi:10.3969/j.issn.1000-4440.2017.06.004]
　HUANG Fang,XU Zhen-zhen,MENG Shan,et al.The identification of long terminal repeat retrotransposons (LTR-RTs) with transcription activity under salt stress and its application in screening the candidate genes related to salt-tolerant in cotton[J].,2017,(01):1220.[doi:doi:10.3969/j.issn.1000-4440.2017.06.004]
[6]王旭明,赵夏夏,陈景阳,等.盐胁迫下水稻孕穗期SS和SPS活性与糖积累的响应及其相关性分析[J].江苏农业学报,2018,(03):481.[doi:doi:10.3969/j.issn.1000-4440.2018.03.001]
　WANG Xu-ming,ZHAO Xia-xia,CHEN Jing-yang,et al.The response and correlations between carbohydrate accumulation and activities of SPS, SS at booting stage of rice under salt stress[J].,2018,(01):481.[doi:doi:10.3969/j.issn.1000-4440.2018.03.001]
[7]李敏,郭聪,李玉娟,等.旱柳转录组测序及生物学分析[J].江苏农业学报,2019,(02):271.[doi:doi:10.3969/j.issn.1000-4440.2019.02.005]
　LI Min,GUO Cong,LI Yu-juan,et al.Transcriptome sequencing and biological analysis of willow (Salix matsudana)[J].,2019,(01):271.[doi:doi:10.3969/j.issn.1000-4440.2019.02.005]
[8]束晓春,李乃伟,汤兴利,等.NaCl处理对不同珊瑚菜种源光合生理和药用有效成分的影响[J].江苏农业学报,2019,(04):790.[doi:doi:10.3969/j.issn.1000-4440.2019.04.006]
　SHU Xiao chun,LI Nai wei,TANG Xing li,et al.Effects of NaCl stress on photosynthetic physiology and active component of different Glehnia littoralis provenance[J].,2019,(01):790.[doi:doi:10.3969/j.issn.1000-4440.2019.04.006]
[9]王永竹,徐为民,杨康,等.H2S对硒胁迫下不结球白菜体内BrPAO1/H2O2的调控作用[J].江苏农业学报,2019,(04):887.[doi:doi:10.3969/j.issn.1000-4440.2019.04.020]
　WANG Yong zhu,XU Wei min,YANG Kang,et al.Regulation of H2S on BrPAO1/H2O2 under selenium stress in Brassica rapa[J].,2019,(01):887.[doi:doi:10.3969/j.issn.1000-4440.2019.04.020]
[10]王馨,闫永庆,殷媛,等.外源γ-氨基丁酸(GABA)对盐胁迫下西伯利亚白刺光合特性的影响[J].江苏农业学报,2019,(05):1032.[doi:doi:10.3969/j.issn.1000-4440.2019.05.005]
　WANG Xin,YAN Yong-qing,YIN Yuan,et al.Effect of exogenous γ-aminobutyric acid(GABA) on photosynthetic characteristics of Nitraria sibirica pall under salt stress[J].,2019,(01):1032.[doi:doi:10.3969/j.issn.1000-4440.2019.05.005]

备注/Memo

备注/Memo:
收稿日期:2023-01-08基金项目:甘肃省自然科学基金项目(21JR11RM042)；甘肃省高等学校创新基金项目(2021B-273)；陇东学院博士基金计划项目(XYBY202007)作者简介:王志科(1988-),男,宁夏吴忠人,博士,副教授,主要从事植物逆境抗性研究。(E-mail)378487542@qq.com
更新日期/Last Update: 2024-03-17