[1]徐彦刚,姚协丰,李良俊,等.西瓜品种（品系）对蔓枯病的抗性鉴定与评价[J].江苏农业学报,2021,(01):106-115.[doi:doi:10.3969/j.issn.1000-4440.2021.01.014]
　XU Yan-gang,YAO Xie-feng,LI Liang-jun,et al.Identification and evaluation of the resistance to gummy stem blight caused by Stagonosporopsis citrulli of some watermelon varieties (strains)[J].,2021,(01):106-115.[doi:doi:10.3969/j.issn.1000-4440.2021.01.014]
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西瓜品种（品系）对蔓枯病的抗性鉴定与评价()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
106-115
栏目:
园艺
出版日期:
2021-02-28

文章信息/Info

Title:
Identification and evaluation of the resistance to gummy stem blight caused by Stagonosporopsis citrulli of some watermelon varieties (strains)
作者:
徐彦刚1; 2; 姚协丰1; 李良俊2; 徐锦华1; 任润生1; 张曼1; 刘广1; 娄丽娜1; 朱凌丽1; 侯茜1; 徐建1; 羊杏平1
（1.江苏省农业科学院蔬菜研究所，江苏南京210014；2.扬州大学园艺与植物保护学院，江苏扬州225009）
Author(s):
XU Yan-gang1; 2; YAO Xie-feng1; LI Liang-jun2; XU Jin-hua1; REN Run-sheng1; ZHANG Man1; LIU Guang1; LOU Li-na1; ZHU Ling-li1; HOU Qian1; XU Jian1
（1.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014，China;2.College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009，China）
关键词:
西瓜; 蔓枯病; 亚隔孢壳菌（Stagonosporopsis citrulli）; 抗性评价
Keywords:
watermelon; gummy stem blight; Stagonosporopsis citrulli; resistance evaluation
分类号:
S651
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.014
文献标志码:
A
摘要:
为探究不同西瓜种质资源对蔓枯病的抗性特性，试验通过对80份不同地域来源的西瓜种质资源进行蔓枯病抗性鉴定，并利用SSR标记技术对该材料群体进行基因分型和群体结构分析。结果表明，供试80份材料对亚隔孢壳菌（Stagonosporopsis citrulli）均无免疫性，但不同西瓜品种(品系)间对蔓枯病抗性却存在较大差异，采用欧式距离进行病情指数聚类分析，可将供试西瓜材料依据蔓枯病病情指数分为高抗材料、抗性材料、中抗材料、感病材料和高感材料5组，其中筛选到高抗材料2份，平均病情指数分别为7.13和8.01； 抗性材料19份，平均病情指数23.04～40.10；中抗材料 21份，平均病情指数41.56～57.74; 感病材料24份，平均病情指数59.14～72.33; 高感材料14份，平均病情指数75.03～84.46。基于贝叶斯统计划分的遗传群体构成分析结果可知，4个不同亚群在病级类型和病情指数这2个特性上存在一定差异，其中亚群POP4整体抗病性水平最高，且抗性材料的占比达到33.34%，而亚群POP2抗性材料占比最低。
Abstract:
To explore the resistant characteristics of different watermelon germplasm resources to gummy stem blight caused by Stagonosporopsis citrulli, 80 watermelon germplasm resources from different regions were used to evaluate their resistance, and simple sequence repeat (SSR) was used to analyze the genotypes and population structure of the samples. The results showed that none of the 80 materials showed immunity to Stagonosporopsis citrulli, but there was difference of the resistance to gummy stem blight between different watermelon varieties (strains). The testing watermelon materials could be divided into five groups, consisting of highly resistant material, resistant material, medium resistant material, susceptible material and highly susceptible material based on the disease index of gummy stem blight, using Euclidean distance in cluster analysis of disease index. Two highly resistant materials were identified with the average disease index of 7.13 and 8.01 respectively, 19 resistant materials were identified with the average disease index distributed between 23.04 and 40.10, 21 medium resistant materials were identified with the average disease index distributed between 41.56 and 57.74, 24 susceptible materials were identified with the average disease index distributed between 59.14 and 72.33, 14 highly susceptible materials were identified with the average disease index distributed between 75.03 and 84.46. The analysis results of the genetic population structure divided by Bayesian statistics showed that there were some differences in disease level types and disease indices in the four different subgroups. The overall disease resistant level of POP4 subgroup was the highest and resistant materials accounted for 33.34%, but the proportion of resistant materials in POP2 subgroup was the lowest.

参考文献/References:

[1]杜少平,马忠明,薛亮. 氮磷钾配施对砂田西瓜产量和品质的影响[J]. 植物营养与肥料学报, 2016, 22(2): 468-475.
[2]马艳,赵江涛,常志州,等. 西瓜内生枯草芽孢杆菌BS211的拮抗活性及盆栽防效[J]. 江苏农业学报, 2006, 22(4): 388-393.
[3]王琛,吴敬学,杨艳涛. 世界西瓜生产和贸易分析及对中国的启示[J]. 农业展望, 2015(2): 71-76.
[4]LI H X, GOTTILLA T M, BREWER M T, et al. Organization and evolution of mating-type genes in three Stagonosporopsis species causing gummy stem blight of cucurbits and leaf spot and dry rot of papaya[J]. Fungal Biology, 2017, 121(10):849-857.
[5]LI H X, BREWER M T. Spatial genetic structure and population dynamics of gummy stem blight fungi within and among watermelon fields in the southeastern United States [J]. Phytopathology, 2016, 106(8):900-908.
[6]ZHAO Q, WU J Z, ZHANG L Y, et al. Identification and characterization of Cucurbita gummy stem blight fungi in Northeast China [J]. Journal of Phytopathology, 2018, 2:1-9.
[7]STEWART J E, TURNER A N, BREWER M T. Evolutionary history and variation in host range of three Stagonosporopsis species causing gummy stem blight of cucurbits [J]. Fungal Biology, 2015, 119: 370-382.
[8]HUANG C J，LAI Y R. First report of Stagonosporopsis citrulli causing gummy stem blight of watermelon in Taiwan[J]. Journal of Plant Pathology, 2018, 101(2): 417.
[9]RENNBERGER G, CLEMSON U, COASTAL R, et al. Occurrence of foliar pathogens of watermelon on commercial farms in south carolina estimated with stratified cluster sampling[J]. Plant Disease, 2018, 102: 2285-2295.
[10]GUSMINI G, SONG R H, WEHNER T C. New sources of resistance to gummy stem blight in watermelon [J]. Crop Science, 2005, 45(2):582-588.
[11]毕研飞,徐兵划,郭静,等. 分子标记辅助甜瓜抗蔓枯病基因聚合及白皮脆品种改良[J]. 南京农业大学学报, 2015, 38(3): 375-380.
[12]马华,程春燕,徐建,等. 黄瓜-酸黄瓜渐渗系的验证及其抗蔓枯病筛选[J]. 南京农业大学学报, 2015, 38(3): 369-374.
[13]LIU S L, SHI Y X, MIAO H, et al. Genetic analysis and QTL mapping of resistance to gummy stem blight in Cucumis sativus seedling stage [J]. Plant Disease, 2017, 101(7): 1-8.
[14]FRANTZ J D, JAHN M M. Five independent loci each control monogenic resistance to gummy stem blight in melon (Cucumis melo L.) [J]. Theoretical and Applied Genetics, 2004, 108(6): 1033-1038.
[15]ZHANG S P, LIU S L, MIAO H, et al. Inheritance and QTL mapping of resistance to gummy stem blight in cucumber stem [J]. Molecular Breeding, 2017, 37(4): 49.
[16]LI P F, REN R S, YAO X F, et al. Identification and characterization of the causal agent of gummy stem blight from muskmelon and watermelon in East China [J]. Journal of Phytopathology, 2015, 163：314-319.
[17]张宁,毕研飞,郭静,等. 不同抗性甜瓜接种蔓枯病菌后PAL、PPO与POD活性的变化[J]. 植物生理学报, 2016, 52(8): 1169-1175.
[18]EVANNO G, REGNAUT S, GOUDET J, et al. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study[J]. Molecular Ecology, 2005, 14: 2611–2620.
[19]赵卫星,常高正,徐小利,等. 西瓜主要病害及抗病育种研究进展[J]. 江西农业学报, 2010, 22(7): 75-78.
[20]马华,程春燕,徐建,等. 黄瓜-酸黄瓜渐渗系的验证及其抗蔓枯病筛选[J]. 南京农业大学学报, 2015, 38(3): 369-374.
[21]LIU S L, SHI Y X, MIAO H, et al. Genetic analysis and QTL mapping of resistance to gummy stem blight in Cucumis sativus seedling stage[J]. Plant Disease, 2017, 101(7): 1-8.
[22]ZHANG S P, LIU S L, MIAO H, et al. Inheritance and QTL mapping of resistance to gummy stem blight in cucumber stem [J]. Molecular Breeding, 2017, 37(4): 49.
[23]顾卫红,杨红娟,马坤,等. 西瓜种质资源的抗蔓枯病性鉴定及其利用[J]. 上海农业学报, 2004, 20(1): 65-67.

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备注/Memo

备注/Memo:
收稿日期：2020-05-24基金项目：国家西甜瓜产业技术体系建设项目(CARS-NO.25-8)作者简介：徐彦刚 (1992-)，男，甘肃平凉人，硕士研究生，主要从事西瓜抗病育种技术研究。（E-mail）1340111782@qq.com通讯作者：羊杏平，（E-mail）xingping@jaas.ac.cn
更新日期/Last Update: 2021-03-15