[1]邱爽,张军,何佳琦,等.大豆GmGolS2-1基因高温胁迫诱导表达及转基因烟草鉴定[J].江苏农业学报,2021,(01):38-43.[doi:doi:10.3969/j.issn.1000-4440.2021.01.005]
　QIU Shuang,ZHANG Jun,HE Jia-qi,et al.Expression of soybean GmGolS2-1 induced by heat stress and identification of GmGolS2-1 transgenic tobacco[J].,2021,(01):38-43.[doi:doi:10.3969/j.issn.1000-4440.2021.01.005]
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大豆GmGolS2-1基因高温胁迫诱导表达及转基因烟草鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
38-43
栏目:
遗传育种·生理生化
出版日期:
2021-02-28

文章信息/Info

Title:
Expression of soybean GmGolS2-1 induced by heat stress and identification of GmGolS2-1 transgenic tobacco
作者:
邱爽1; 张军2; 何佳琦1; 李铭杨1; 周雨明3; 邬长乐1; 袁洪淼1; 刘嘉仪1; 翟莹1
(1.齐齐哈尔大学生命科学与农林学院，黑龙江齐齐哈尔161006；2.黑龙江省农业科学院畜牧兽医分院，黑龙江齐齐哈尔161005；3.吉林中智九方咨询有限公司，吉林长春130000)
Author(s):
QIU Shuang1; ZHANG Jun2; HE Jia-qi1; LI Ming-yang1; ZHOU Yu-ming3; WU Chang-le1; YUAN Hong-miao1; LIU Jia-yi1; ZHAI Ying1
(1.College of Life Science and Agro-Forestry, Qiqihar University, Qiqihar 161006, China;2.Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005, China;3.Jilin Zhongzhi Jiufang Consulting Co., Ltd., Changchun 130000, China)
关键词:
大豆; 肌醇半乳糖苷; GolS基因; 高温胁迫; 转基因烟草
Keywords:
soybean; galactinol; GolS gene; heat stress; transgenic tobacco
分类号:
S565.1
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.005
文献标志码:
A
摘要:
肌醇半乳糖苷合成酶（GolS）是棉籽糖系列寡糖（RFO）生物合成途径中的关键酶，在植物应对非生物胁迫过程中发挥重要作用。实时荧光定量RT-PCR结果显示，高温胁迫可以诱导GmGolS2-1在大豆幼苗中的表达。将GmGolS2-1基因构建到植物表达载体pRI101上并通过叶盘法转化烟草，经卡那霉素抗性筛选，PCR及qRT-PCR检测共获得6株阳性转基因烟草植株（OE1～OE6）。对野生型烟草植株和GmGolS2-1转基因烟草植株进行高温胁迫处理，结果显示野生型烟草的电解质渗透率和丙二醛含量均高于转基因烟草。由此推测GmGolS2-1可以提高转基因烟草的耐热性。
Abstract:
Galactinol synthase (GolS) is the key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), which plays an important role in the response to abiotic stresses of plants. The results of real-time fluorescence quantitative RT-PCR showed that the expression of GmGolS2-1 could be induced by high temperature stress in soybean seedlings. The GmGolS2-1 gene was constructed into expression vector pRI101 in plants and was transformed into tobacco using leaf disc method. Six positive transgenic tobacco plants (OE1-OE6) were obtained by kanamycin resistance screening, PCR and qRT-PCR. The wild-type and GmGolS2-1 transgenic tobacco plants were treated with heat stress. The results showed that the electrolyte leakage and malondialdehyde content of wild-type tobacco were both higher than that of transgenic tobacco. These data indicate that GmGolS2-1 can increase the tolerance to heat stress of transgenic tobacco.

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备注/Memo

备注/Memo:
收稿日期：2020-06-29基金项目：黑龙江省普通本科高等学校青年创新人才培养计划项目（UNPYSCT-2017153）；黑龙江省省属高等学校基本科研业务费科研项目（植物性食品加工技术特色学科专项）（YSTSXK201878）；黑龙江省省属高等学校基本科研业务费科研项目（135209264）；齐齐哈尔大学研究生创新科研项目（YJSCX2019050）作者简介：邱爽（1995-），男，山东济宁人，硕士研究生，主要从事大豆分子育种研究。(E-mail)qs187143@163.com通讯作者：翟莹，(Tel)15845640163；(E-mail)fairy39809079@126.com
更新日期/Last Update: 2021-03-15