[1]肖琦,赵霞玲,钱雯娴,等.猪链球菌3型分离菌的生物学特性及致病性[J].江苏农业学报,2020,(06):1475-1481.[doi:doi:10.3969/j.issn.1000-4440.2020.06.017]
　XIAO Qi,ZHAO Xia-ling,QIAN Wen-xian,et al.Biological characteristics and pathogenicity of Streptococcus suis serotype 3 isolates[J].,2020,(06):1475-1481.[doi:doi:10.3969/j.issn.1000-4440.2020.06.017]
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猪链球菌3型分离菌的生物学特性及致病性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年06期
页码:
1475-1481
栏目:
畜牧兽医·水产养殖
出版日期:
2020-12-31

文章信息/Info

Title:
Biological characteristics and pathogenicity of Streptococcus suis serotype 3 isolates
作者:
肖琦1; 赵霞玲1; 2; 钱雯娴1; 2; 郭佳慧1; 3; 檀济敏1; 4; 俞正玉1; 汪伟1; 孙珂1; 5; 倪艳秀1; 祝昊丹1; 周俊明1; 姚火春4
(1.江苏省农业科学院兽医研究所，农业部兽用生物制品工程技术重点实验室，江苏南京210014；2.西藏农牧学院，西藏林芝860000；3.扬州大学兽医学院，江苏扬州225009；4.南京农业大学动物医学院，江苏南京210095；5.吉林农业大学动物科学技术学院，吉林长春130118)
Author(s):
XIAO Qi1; ZHAO Xia-ling1; 2; QIAN Wen-xian1; 2; GUO Jia-hui1; 3; TAN Ji-min1; 4; YU Zheng-yu1; WANG Wei1; SUN Ke1; 5; NI Yan-xiu1; ZHU Hao-dan1; ZHOU Jun-ming1; YAO Huo-chun4; FAN Hong-jie4; NIU Jia-qiang2; SUOLANG Si-zhu2; HE Kong-wang1
(1.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China;2.Tibet College of Agriculture and Animal Husbandry, Linzhi 860000, China;3.College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;4.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China；5.College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China)
关键词:
猪链球菌3型; 生物学特性; 致病性
Keywords:
Streptococcus suis serotype 3; biological characteristics; pathogenicity
分类号:
S855.1+1
DOI:
doi:10.3969/j.issn.1000-4440.2020.06.017
文献标志码:
A
摘要:
为研究猪链球菌3型(Ss3)分离菌的生物学特性及致病性，对疑似Ss进行培养、染色、生化试验、猪链球菌PCR鉴定、血清分型PCR鉴定、多位点序列分型(MLST型)PCR鉴定，并对小鼠和仔猪进行致病性试验。结果显示，3株分离菌(分别命名为JSHZ菌株、GD7菌株、H30菌株)均呈革兰氏阳性，Ss PCR鉴定显示为阳性，血清分型PCR结果表明分离到的菌株为Ss3，序列型(ST)分别属于ST27型(JSHZ菌株)、ST1004型(GD7菌株)和ST 117型(H30菌株)；3株分离菌的生化特性与猪链球菌相符；3株分离菌经腹腔注射均可致死Balb/C小鼠，半致死量(LD50)分别为2.0×107CFU/ml、3.5×107CFU/ml和2.5×108CFU/ml。选择3株分离菌中毒力较强的JSHZ菌株进行仔猪致病性试验，感染仔猪会出现明显的关节红肿、跛行，体温多数升高至40.5～41.8 ℃，精神萎靡，食欲减少等症状；高剂量(每头仔猪注射JSHZ菌数6.0×109 CFU)处理可致仔猪出现食欲废绝、神经症状，甚至濒死，并在其脑组织中分离到Ss3。本研究结果为进一步开展Ss3的防控研究奠定了基础。
Abstract:
In order to study the biological characteristics and pathogenicity of Streptococcus suis serotype 3(Ss3) isolates, three strains of suspected Streptococcus suis (Ss), strain JSHZ and GD7 isolated from acute dead piglets, strain H30 isolated from healthy piglet, were cultured, stained and identified by Ss PCR, Ss serotyping PCR, multilocus sequence typing (MLST) PCR, biochemical tests, and pathogenicity tests were conducted on mice and piglets. The results showed that all the three isolates were Gram-positive and Ss3. The sequence type (ST) of JSHZ, GD7 and H30 belonged to ST27, ST1004 and ST117, respectively. The biochemical characteristics of the three strains were consistent with Ss. The three strains could be lethal to mice by intraperitoneal injection, and the median lethal dose (LD50) were 2.0×107CFU/ml(JSHZ), 3.5×107CFU/ml(GD7) and 2.5×108CFU/ml(H30), respectively. The JSHZ with highest virulence to mice was selected for the pathogenicity test on piglets. The results showed that for piglets, JSHZ could cause obvious arthritis and limping, raise the body temperature to 40.5-41.8 ℃, reduce the spirit and appetite. High dose of JSHZ(6.0×109 CFU) could cause piglet anorexia, neurological symptoms and even being dying, and Ss3 was isolated from the brain tissues of dying piglet. This study can lay a foundation for further research on prevention and control of Ss3.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2020-08-27基金项目：省部共建国家重点实验室培育基地江苏省食品质量安全重点实验室项目(2019SY001)；江苏现代农业(生猪)产业技术体系生物安全创新团队项目[JATS(2018)259]；国家公益性行业(农业)科研专项(201303041)作者简介：肖琦(1980-)，女，辽宁丹东人，博士，助理研究员，主要从事猪病及人兽共患病防控研究。(E-mail)xiaoqi_2122@163.com通讯作者：何孔旺，(E-mail)kwh2003@263.net
更新日期/Last Update: 2021-01-15