[1]陈盛,王宁宁,王玉康,等.一种快速高效筛选甘蓝型油菜转化植株的方法[J].江苏农业学报,2017,(05):982-985.[doi:doi:10.3969/j.issn.1000-4440.2017.05.004]
　CHEN Sheng,WANG Ning-ning,WANG Yu-kang,et al.A rapid and efficient approach to screening transformed plants of Brassica napus[J].,2017,(05):982-985.[doi:doi:10.3969/j.issn.1000-4440.2017.05.004]
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一种快速高效筛选甘蓝型油菜转化植株的方法()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
982-985
栏目:
遗传育种·生理生化
出版日期:
2017-10-30

文章信息/Info

Title:
A rapid and efficient approach to screening transformed plants of Brassica napus
作者:
陈盛1; 王宁宁1; 王玉康1; 陈松2; 曹维1; 李明1; 朱克明1; 彭琦2; 王政1; 谭小力1
（1.江苏大学生命科学研究院，江苏镇江212013;2.江苏省农业科学院经济作物研究所，江苏南京210014）
Author(s):
CHEN Sheng1; WANG Ning-ning1; WANG Yu-kang1; CHEN Song2; CAO Wei1; LI Ming1; ZHU Ke-ming1; PENG Qi2; WANG Zheng1; TAN Xiao-li1
(1.Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China;2.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
甘蓝型油菜; 转基因; 转化植株筛选
Keywords:
Brassica napus; gene transformation; screening of transformed plant
分类号:
S565.403.53
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.004
文献标志码:
A
摘要:
为了能够快速高效地筛选出转化的油菜植株，采用农杆菌介导的转基因技术将含有绿色荧光蛋白(GFP)的激活标签载体转化到甘蓝型油菜中，对得到的T1代油菜种子进行萌发，长出约3 mm胚芽后，通过直接观察绿色荧光的筛选方法得到转基因植株。筛选出的植株经PCR验证，阳性率高达75.37%。该筛选方法具有用时短、效率高、易操作等优点，适用于甘蓝型油菜转化植株的筛选工作。
Abstract:
In order to rapidly and efficiently screen the transformed plants of Brassica napus, Agrobacterium tumefaciens-mediated transgenic technique was used to transform the activated marker vector containing GFP (green fluorescent protein) into B. napus. Three-milimeter embryos grew from T1 generation seeds, and the transgenic plants were obtained by direct observation of green fluorescence. PCR confirmed the positive rate of transgenic plants was 75.37%. The screening method has the advantages of short time, high efficiency and easy operation, and is suitable for the screening of transformed plants of B. napus.

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备注/Memo

备注/Memo:
收稿日期：2017-03-03 基金项目：国家重点研发计划项目（2016YFD0101904）; 国家自然科学基金面上项目（31471527） 作者简介：陈盛（1991-），男，安徽宣城人，硕士研究生，研究方向为油菜生物技术。（E-mail）schenxcgq@sina.com 通讯作者：谭小力，（E-mail）xltan@ujs.edu.cn
更新日期/Last Update: 2017-11-03